Functional role of proteolytic processing of recombinant myocilin in self-aggregation

Investigative Ophthalmology and Visual Science

Volumn 51, Issue 1, 2010, Pages 72-78

  Aroca Aguilar, José Daniel ^a,b   Martínez Redondo, Francisco ^a,b   Sánchez Sánchez, Francisco ^a,b   Coca Prados, Miguel ^c   Escribano, Julio ^a,b  

^a Universidad de Castilla La Mancha   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MYOCILIN; RECOMBINANT PROTEIN; CYTOSKELETON PROTEIN; DISULFIDE; EYE PROTEIN; GLYCOPROTEIN; TRABECULAR MESHWORK-INDUCED GLUCOCORTICOID RESPONSE PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELL STRAIN HEK293; CONTROLLED STUDY; COVALENT BOND; COW; DISULFIDE BOND; EMBRYO; HUMAN; HUMAN CELL; IONIC STRENGTH; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; ANIMAL; ANTERIOR EYE SEGMENT; CATTLE; CELL LINE; CHEMISTRY; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KIDNEY; METABOLISM; OSMOLARITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRENATAL DEVELOPMENT; PROTEIN BINDING; SCLERA; WESTERN BLOTTING;

ANIMALS; ANTERIOR EYE SEGMENT; BLOTTING, WESTERN; CATTLE; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOSKELETAL PROTEINS; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EYE PROTEINS; GENE EXPRESSION; GLYCOPROTEINS; HUMANS; KIDNEY; OSMOLAR CONCENTRATION; PROTEIN BINDING; RECOMBINANT PROTEINS; SCLERA; TRANSFECTION;

EID: 75749084513     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.09-4118     Document Type: Article

References (36)

1. Escribano J, Ortego J, Coca-Prados M. Isolation and characterization of cell-specific cDNA clones from a subtractive library of the ocular ciliary body of a single normal human donor: transcription and synthesis of plasma proteins. J Biochem (Tokyo). 1995;118: 921-931.
2. Kubota R, Noda S, Wang Y, et al. A novel myosin-like protein (myocilin) expressed in the connecting cilium of the photoreceptor: molecular cloning, tissue expression, and chromosomal mapping. Genomics. 1997;41:360-369.
3. Ortego J, Escribano J, Coca-Prados M. Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR, a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin. FEBS Lett. 1997;413:349-353.
4. Adam MF, Belmouden A, Binisti P, et al. Recurrent mutations in a single exon encoding the evolutionarily conserved olfactomedinhomology domain of TIGR in familial open-angle glaucoma. Hum Mol Genet. 1997;6:2091-2097.
5. Huang W, Jaroszewski J, Ortego J, Escribano J, Coca-Prados M. Expression of the TIGR gene in the iris, ciliary body, and trabecular meshwork of the human eye. Ophthalmic Genet. 2000;21:155-169.
6. Karali A, Russell P, Stefani FH, Tamm ER. Localization of myocilin/ trabecular meshwork: inducible glucocorticoid response protein in the human eye. Invest Ophthalmol Vis Sci. 2000;41:729-740.
7. Fautsch MP, Johnson DH. Characterization of myocilin-myocilin interactions. Invest Ophthalmol Vis Sci. 2001;42:2324-2331.
8. Russell P, Tamm ER, Grehn FJ, Picht G, Johnson M. The presence and properties of myocilin in the aqueous humor. Invest Ophthalmol Vis Sci. 2001;42:983-986.
9. Fautsch MP, Vrabel AM, Peterson SL, Johnson DH. In vitro and in vivo characterization of disulfide bond use in myocilin complex formation. Mol Vis. 2004;10:417-425.
10. Stone EM, Fingert JH, Alward WL, et al. Identification of a gene that causes primary open angle glaucoma. Science. 1997;275:668-670.
11. Fingert JH, Heon E, Liebmann JM, et al. Analysis of myocilin mutations in 1703 glaucoma patients from five different populations. Hum Mol Genet. 1999;8:899-905.
12. Campos-Mollo E, Sanchez-Sanchez F, Lopez-Garrido MP, Lopez-Sanchez E, Lopez-Martinez F, Escribano J. MYOC gene mutations in Spanish patients with autosomal dominant primary open-angle glaucoma: a founder effect in southeast Spain. Mol Vis. 2007;13: 1666-1673.
13. Quigley HA. Neuronal death in glaucoma. Prog Retin Eye Res. 1999;18:39-57.
14. Mukhopadhyay A, Gupta A, Mukherjee S, Chaudhuri K, Ray K. Did myocilin evolve from two different primordial proteins? Mol Vis. 2002;8:271-279.
15. Aroca-Aguilar JD, Sanchez-Sanchez F, Ghosh S, Coca-Prados M, Escribano J. Myocilin mutations causing glaucoma inhibit the intracellular endoproteolytic cleavage of myocilin between amino acids Arg226 and Ile227. J Biol Chem. 2005;280:21043-21051.
16. Kanagavalli J, Krishnadas SR, Pandaranayaka E, Krishnaswamy S, Sundaresan P. Evaluation and understanding of myocilin mutations in Indian primary open angle glaucoma patients. Mol Vis. 2003;9: 606-614.
17. Stamer WD, Perkumas KM, Hoffman EA, Roberts BC, Epstein DL, McKay BS. Coiled-coil targeting of myocilin to intracellular membranes. Exp Eye Res. 2006;83:1386-1395.
18. Furutani Y, Manabe R, Tsutsui K, et al. Identification and characterization of photomedins: novel olfactomedin-domain-containing proteins with chondroitin sulphate-E-binding activity. Biochem J. 2005;389:675-684.
19. Maertens B, Hopkins D, Franzke CW, et al. Cleavage and oligomerization of gliomedin, a transmembrane collagen required for node of ranvier formation. J Biol Chem. 2007;282:10647-10659.
20. Li Y, Aroca-Aguilar JD, Ghosh S, Sanchez-Sanchez F, Escribano J, Coca-Prados M. Interaction of myocilin with the C-terminal region of hevin. Biochem Biophys Res Commun. 2006;339:797-804.
21. Sanchez-Sanchez F, Martinez-Redondo F, Aroca-Aguilar JD, Coca-Prados M, Escribano J. Characterization of the intracellular proteolytic cleavage of myocilin and identification of calpain II as a myocilin-processing protease. J Biol Chem. 2007;282:27810-27824.
22. Switzer RC III, Merril CR, Shifrin S. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem. 1979;98:231-237.
23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem. 1976;72:248-254.
24. Filla MS, Liu X, Nguyen TD, et al. In vitro localization of TIGR/ MYOC in trabecular meshwork extracellular matrix and binding to fibronectin. Invest Ophthalmol Vis Sci. 2002;43:151-161.
25. McCloskey N, Turner MW, Goldblatt TD. Correlation between the avidity of mouse-human chimeric IgG subclass monoclonal antibodies measured by solid-phase elution ELISA and biospecific interaction analysis (BIA). J Immunol Methods. 1997;205:67-72.
26. Hall RA. Studying protein-protein interactions via blot overlay or Far Western blot. Methods Mol Biol. 2004;261:167-174.
27. Nagy I, Trexler M, Patthy L. Expression and characterization of the olfactomedin domain of human myocilin. Biochem Biophys Res Commun. 2003;302:554-561.
28. Coca-Prados M, Escribano J. New perspectives in aqueous humor secretion and in glaucoma: the ciliary body as a multifunctional neuroendocrine gland. Prog Retin Eye Res. 2007;26:239-262.
29. Gobeil S, Letartre L, Raymond V. Functional analysis of the glaucoma-causing TIGR/myocilin protein: integrity of amino-terminal coiled-coil regions and olfactomedin homology domain is essential for extracellular adhesion and secretion. Exp Eye Res. 2006;82: 1017-1029.
30. Gobeil S, Rodrigue MA, Moisan S, et al. Intracellular sequestration of hetero-oligomers formed by wild-type and glaucoma-causing myocilin mutants. Invest Ophthalmol Vis Sci. 2004;45:3560-3567.
31. Snyder DA, Rivers AM, Yokoe H, Menco BP, Anholt RR. Olfactomedin: purification, characterization, and localization of a novel olfactory glycoprotein. Biochemistry. 1991;30:9143-9153.
32. Yokoe H, Anholt RR. Molecular cloning of olfactomedin, an extracellular matrix protein specific to olfactory neuroepithelium. Proc Natl Acad Sci U S A. 1993;90:4655-4659.
33. Moreno TA, Bronner-Fraser M. The secreted glycoprotein Noelin-1 promotes neurogenesis in Xenopus. Dev Biol. 2001;240:340-360.
34. Ezzat MK, Howell KG, Bahler CK, et al. Characterization of monoclonal antibodies against the glaucoma-associated protein myocilin. Exp Eye Res. 2008;87:376-384.
35. Fautsch MP, Bahler CK, Vrabel AM, et al. Perfusion of his-tagged eukaryotic myocilin increases outflow resistance in human anterior segments in the presence of aqueous humor. Invest Ophthalmol Vis Sci. 2006;47:213-221.
36. Zhou Y, Grinchuk O, Tomarev SI. Transgenic mice expressing the Tyr437His mutant of human myocilin protein develop glaucoma. Invest Ophthalmol Vis Sci. 2008;49:1932-1939.