A polymerase chain reaction assay for the detection of xanthomonas campestris pv. musacearum in Banana

Plant Disease

Volumn 94, Issue 1, 2010, Pages 109-114

  Ivey, Melanie L Lewis ^a   Tusiime, Geoffrey ^b   Miller, Sally A ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b MAKERERE UNIVERSITY   (Uganda)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLICONS; CAUSATIVE AGENTS; GENE CLUSTERS; GENOMIC DNA; TANZANIA; WHOLE CELL; XANTHOMONAS; XANTHOMONAS CAMPESTRIS;

BACTERIOLOGY; GENES; POLYMERIZATION;

POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; XANTHOMONAS; XANTHOMONAS AXONOPODIS; XANTHOMONAS CAMPESTRIS; XANTHOMONAS VASICOLA; ZEA MAYS;

EID: 75649151510     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS-94-1-0109     Document Type: Article

References (62)

1. Alfano, J. R., and Collmer, A. 1997. The type III (Hrp) secretion pathway of plant pathogenic bacteria: trafficking harpins, Avr proteins, and death. J. Bacteriol. 179:5655-5662.
2. Aritua, V., Nanyonjo, A., Kumakech, F., and Tushemereirwe, W. 2008. Rep-PCR reveals a high genetic homogeneity among Ugandan isolates of Xanthomonas campestris pv. musacearum. Afr. J. Biotechnol. 6:179-183.
3. Aritua V., Parkinson, N., Thwaites, R., Heeney, J. V., Jones, D. R., Tushemereirwe, W., Crozier, J., Reeder, R., Stead, D. E., and Smith, J. 2008. Characterization of the Xanthomonas sp. causing bacterial wilt of enset and banana and its proposed reclassification as a strain of X. vasicola. Plant Pathol. 57:170-177.
4. Bafana, B. 2008. Trade-East Africa: Going Bananas to Fight Poverty and Hunger. IPS Inter Press Service News Agency (IPS). http://ipsnews.net/news.asp? idnews=44210. Accessed October 2008.
5. Bereswill, S., Pahl, A., Bellemann, P., Zeller, W., and Geider, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Appl. Environ. Microbiol. 58:3522-3526.
6. Berg, T., Tesoriero, L., and Hailstones, D. L. 2005. PCR-based detection of Xanthomonas campestris pathovars in Brassica seed. Plant Pathol. 54:416-427.
7. Biruma, M., Pillay, M., Tripathi, L., Blomme, G., Abele, S., Mwangi, M., Bandyopadhyay, R., Muchunguzi, P., Kassim, S., Nyine, M., Turyagyenda, L., and Eden-Green, S. 2007. Banana Xanthomonas wilt: a review of the disease, management strategies and future research directions. Afr. J. Biotechnol. 6:953-962.
8. Chen, J., Groves, R., Civerolo, E. L., Viveros, M., Freeman, M., and Zheng, Y. 2005. Two Xylella fastidiosa genotypes associated with almond leaf scorch disease on the same location in California. Phytopathology 95:708-714.
9. Cubero, J., and Graham, J. H. 2002. Genetic relationship among world wide strains of Xanthomonas causing canker in citrus species and design of new primers for their identification by PCR. Appl. Environ. Microbiol. 68:1257-1264.
10. Cuppels, D. A., Louws, F. J., and Ainsworth, T. 2006. Development and evaluation of PCR-based diagnostic assays for the bacterial speck and bacterial spot pathogens of tomato. Plant Dis. 90:451-458.
11. Cuppels, D. A., Moore, R. A., and Morris, V. L. 1990. Construction and use of a nonradioactive DNA hybridization probe for detection of Pseudomonas syringae pv. tomato on tomato plants. Appl. Environ. Microbiol. 56:1743-1749.
12. de Bruijn, F. J. 1992. Use of repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Appl. Environ. Microbiol. 58:2180-2187.
13. DeParasis, J., and Roth, D. A. 1990. Nucleic acid probes for identification of phytobacteria: identification of genus-specific 16S rRNA sequences. Phytopathology 80:618-621.
14. Dreier, J., Bermpohl, A., and Eichenlaub, R. 1995. Southern hybridization and PCR for specific detection of phytopathogenic Clavibacter michiganensis subsp. michiganensis. Phytopathology 85:508-512.
15. Dye, D. W., Bradbury, J. F., Goto, M., Hayward, A. C., Lelliot, R. A., and Schroth, M. N. 1980. International standards for naming pathovars of phytopathogenic bacteria: a list of pathovars names and pathotype strains. Rev. Plant Pathol. 59:153-168.
16. Engels, B. 2005. Amplify 3.1X. University of Wisconsin. http://engels.genetics.wisc.edu/amplify/. Accessed September 2006.
17. Ewing, B., and Green, P. 1998. Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 8:186-194 (Pubitemid 28177230)
18. Ewing, B., Hillier, L., Wendl, M. C., and Green, P. 1998. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8:175-185 (Pubitemid 28177229)
19. Food and Agriculture Organization of The United Nations. 2005. Banana Statistics, 2005. http://faostat.fao.org/site/339/default.aspx. Accessed January 2005.
20. Hartung, J. S., Daniel, J. F., and Pruvost, O. P. 1993. Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method. Appl. Environ. Microbiol. 59:1143-1148.
21. Hauben, L., Vauterin, L., Swings, J., and Moore, R. B. 1997. Comparison of 16S ribosomal DNA sequences of all Xanthomonas species. Int. J. Syst. Bacteriol. 47:328-335.
22. Kerkoud, M., Manceau, C., and Paulin, J. P. 2002. Rapid diagnosis of Pseudomonas syringae pv. papulans, the causal agent of blister spot of apple, by polymerase chain reaction using specifically designed hrpL gene primers. Phytopathology 92:1077-1083.
23. Kumar, S., Tamura, K., and Nei, M. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5:150-163.
24. Leite, R P, Jr., Jones, J. B., Somodi, G. C., Minsavage, G. V., and Stall, R. E. 1995. Detection of Xanthomonas campestris pv. vesicatoria associated with pepper and tomato seed by DNA amplification. Plant Dis. 79:917-922.
25. Leite, R P, Jr., Minsavage, G. V., Bonas, U., and Stall, R. E. 1994. Detection and identification of phytopathogenic Xanthomonas strains by amplification of DNA sequences related to the hrp genes of Xanthomonas campestris pv. vesicatoria. Appl. Environ. Microbiol. 60:1068-1077.
26. Lelliot, R. A., and Stead, D. E. 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Blackwell Scientific Publication, Oxford.
27. Li, X., and De Boer, S. H. 1995. Selection of polymerase chain reaction primers from an RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. sepedonicus. Phytopathology 85:837-842.
28. Lindgren, P. B., Peet, R. C., and Panopoulos, N. J. 1986. Gene cluster of Pseudomonas syringae pv. "phaseolicola" controls pathogenicity of bean plants and hypersensitivity on nonhost plants. J. Bacteriol. 168:512-522.
29. Louws, F. J., Fulbright, D. W., and de Bruijn, F. J. 1994. Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl. Environ. Microbiol. 60:2286-2295.
30. Mavrodieva, V., Levy, L., and Gabriel, D. W. 2004. Improved sampling methods for realtime polymerase chain reaction diagnosis of citrus canker from field samples. Phytopathology 94:61-68.
31. Mbaka, J., Ndungu, V., and Mwangi, M. 2007. Outbreak of Xanthomonas wilt (Xanthomonas campestris pv. musacearum) on banana in Kenya. Pages 58-59 in: Program and Abstracts of ISHS/ProMusa Symposium: Recent Advances in Banana Crop Protection for Sustainable Production and Improved Livelihoods. Greenway Woods Resort, White River, South Africa.
32. Mgenzi, S. R. B, Eden-Green S., and Peacock, J. 2006. Overview of banana Xanthomonas wilt in Tanzania. In: Proc. 4th Int. Bacterial Wilt Symp. Central Science Laboratory, York, UK.
33. Ndungo, V., Bakelana, K., Eden-Green, S., and Blomme, G. 2005. An outbreak of banana Xanthomonas wilt in the Democratic Republic of Congo. Infomusa 13:43-44.
34. Ndungo, V., Eden-Green, S., Blomme, G., Crozier, J., and Smith, J. J. 2006. Presence of banana Xanthomonas wilt (Xanthomonas campestris pv. musacearum) in the Democratic Republic of Congo (DRC). Plant Pathol. 55:294.
35. Obradovic, A., Mavridis, A., Rudolph, K., Janse, J. D, Arsenijevic, M., Jones, J. B, Minsavage, G. V., and Wang, J. 2004. Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. Eur. J. Plant Pathol. 11:285-292.
36. Opina, N, Tavner, F., Hollway, G., Wang, J. F., Li, T. H., Maghirang, R., Fegan, M., Hayward, A.C., Krishnapillai, V., Hong, W. F., Holloway, B. W., and Timmins, J. 1997. A novel method for development of species and strain-specific DNA probes and PCR primers for identifying Burkholderia solanacearum (formerly Pseudomonas solanacearum). Asia Pac. J. Mol. Biol. Biotechnol. 5:19-30.
37. Park, Y. J., Lee, B. M., Ho-Hahn, J., Lee, G. B., and Park. D. S. 2004. Sensitive and specific detection of Xanthomonas campestris pv. campestris by PCR using species-specific primers based on hrpF gene sequences. Microbiol. Res. 159:419-423.
38. Parkinson, N., Cowie, C., Heeney, J., and Stead, D. 2009. Phylogenetic structure of Xanthomonas determined by comparison of gyrB sequences. Int. J. Syst. Evol. Microbiol. 59:264-274.
39. Pooler, M. R., and Hartung, S. 1995. Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis. Curr. Microbiol. 31:377-381.
40. Poussier, S., Prior, P., Luisetti, J., Hayward, C., and Fegan, M. 2000. Partial sequencing of the hrpB and endogluconase genes confirms and expands the known diversity within the Ralstonia solanacearum species complex. Syst. Appl. Microbiol. 23:479-486.
41. Prosen, D., Hatziloukas, E., Schaad, N. W., and Panopoulos, N. J. 1993. Specific detection of Pseudomonas syringae pv. phaseolicola DNA in bean seed by polymerase chain reaction-based amplification of a phaseolotoxin gene region. Phytopathology 83:965-970.
42. Reeder, R. H., Muhinyuza, J. B., Opolot, O., Aritua, V., Crozier, J., and Smith, J. 2008. Presence of banana bacterial wilt (Xanthomonas campestris pv. musacearum) in Rwanda. Plant Pathol. 56:1038.
43. Roberts, S. J., Eden-Green, S. J., Jones, P., and Ambler, D. J. 1990. Pseudomonas syzygii sp. nov., the cause of Sumatra disease of cloves. Syst. Appl. Microbiol. 13:34-43.
44. Rodrigues, J. L. M., Silva-Stenico, M. E., Gomes, J. E., Lopes, J. R. S., and Tsai, S. M. 2003. Detection and diversity assessment of Xylella fastidiosa in field-collected plant and insect samples by using 16S rRNA and gyrB sequences. Appl. Environ. Microbiol. 69:4249-4255.
45. Sahin, F., Abbassi, P. A., Lewis Ivey, M. L., Zhang, J., and Miller, S. A. 2003. Diversity among strains of Xanthomonas campestris pv. vitians from lettuce. Phytopathology 93:64-70. (Pubitemid 36029209)
46. Schaad, N. W., and Stall, R. E. 1988. Xanthomonas. Pages 81-94 in: Laboratory Guide for Identification of Plant Pathogenic Bacteria, 2nd ed. N. W. Schaad, ed. APS Press, St. Paul, MN.
47. Schonfeld, J., Heuer, H., van Elas, J. D., and Smalla, K. 2003. Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of fliC fragments. Appl. Environ. Microbiol. 69:7248-7256.
48. Seal, S. E., Jackson, L. A., Young, J. P. W., and Daniels, M. J. 1993. Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the blood disease bacterium by partial 16S rDNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. J. Gen. Microbiol. 139:1587-1594.
49. Smith, J. J., Jones, D. R., Karamura, E., Blomme, G., and Turyagyenda, F. L. 2008. An Analysis of the Risk from Xanthomonas campestris pv. musacearum to Banana Cultivation in Eastern, Central and Southern Africa. Biodiversity International, Montpellier, France.
50. Sousa, S. M., Cruz, L., Norskov, P., and Rasmussen, O. F. 1997. A rapid and sensitive detection of Clavibacter michiganensis subsp. michiganensis in tomato seeds by polymerase chain reaction. Seed Sci. Technol. 25:581-584.
51. Stall., R. E., and Minsavage, G. V. 1990. The use of hrp genes to identify opportunistic xanthomonads. Pages 369-374 in: Proc. 7th Int. Conf. Plant Pathol. Z. Klement, ed. Budapest.
52. Taghavi, M., Hayward, C., Sly, L. I., and Fegan, M. 1996. Analysis of the phylogenetic relationships of strains of Burkholderia solanacearum, Pseudomonas syzygii, and the blood disease bacterium on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 46:10-15.
53. Thwaites, R., Eden-Green, S. J., and Black, R. 2000. Diseases caused by bacteria. Pages 213-240 in: Diseases of Banana: Abaca and Enset, D. R. Jones, ed. CAB International, Wallingford, UK.
54. Tushemereirwe, W., Kangire, A., Smith, J., Ssekiwoko, F., Nakyanzi, M., Kataama, D., Musiitwa, C., and Karyaija, R. 2003. An outbreak of bacterial wilt on banana in Uganda. Infomusa 12:6-8.
55. Tushemereirwe, W., Kangire, Ssekiwoko, F., Offord, L. C., Crozier, J., Boa, E., Rutherford, M., and Smith, J. 2004. First report of Xanthomonas campestris pv. musacearum on banana in Uganda. Plant Pathol. 53:802.
56. Willis, K., Rich, J. J., and Hrabak, E. M. 1990. hrp genes of phytopathogenic bacteria. Mol. Plant-Microbe Interact. 4:132-138.
57. Yirgou, D., and Bradbury, J. F. 1968. Bacterial wilt of Enset (Ensete ventricosa) incited by Xanthomonas musacearum sp. n. Phytopathology 58:111-112.
58. Yirgou, D., and Bradbury, J. F. 1974. A note on wilt of banana caused by the Ensete wilt organism, Xanthomonas musacearum. EA Agric. For. J. 40:111-114.
59. Young, J. M., Bull, C. T., De Boer, S. H., Firrao, G., Gardan, L., Saddler, G. E., Stead, D. E., and Takikawa, Y. 2004. Names of Plant Pathogenic Bacteria Since 1995. Report of the Taxonomy of Bacterial Plant Pathogens Committee of the International Society of Plant Pathology. http://www.isppweb. org/names-bacterial-new2004.asp. Accessed March 2004.
60. Young, J. M., Dye, D. W., Bradbury, J. F., and Panagopoulos, C. G. 1978. A proposed nomenclature and classification for plant pathogenic bacteria. New Zealand J. Agric. Res. 21:153-177.
61. Zaccardelli, M., Campanile, F., Spasiano, A., and Merighi, M. 2007. Detection and identification of the crucifer pathogen, Xanthomonas campestris pv. campestris, by PCR amplification of the conserved Hrp/type III secretion system gene hrcC. Eur. J. Plant Pathol. 118:299-306.
62. pst. Eur. J. Plant Pathol. 111:85-90.