Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding

Biotechnology and Bioprocess Engineering

Volumn 8, Issue 3, 2003, Pages 183-186

  Ramisetti, Srinivas ^a   Kang, Hyun Ah ^a   Rhee, Sang Ki ^a   Kim, Chul Ho ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Fed batch fermentation; GAL promoter; Galactose; Recombinant hirudin; Saccharomyces cerevisiae

Indexed keywords

EID: 7544244720     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02935894     Document Type: Article

References (14)

1. Markwardt, F. (1982) Pharmacology of hirudin: One hundred years after the first report of anticoagulant agent in medicinal leeches. Biomed. Biochem. Acta 44: 1007-1013.
2. Bagdy, D., E. Barabas, L. Graf, T. E. Petersen, and S. Magnusson (1976) Hirudin. Methods Enzymol. 45: 669-678.
3. Markwardt, F., J. Hauptmann, G. Nowak, C. Klessen, and P. Walsman (1985) Pharmacological studies on the antithrombin action of hirudin in experimental animals. Thromb. Haemostasis 47: 226-229.
4. Markwardt, F. (1989) Development of hirudin as an antithrombotic agent. Sem. Thromb. Homeostatis. 15: 269-282.
5. Sohn, J. H., H. A. Kang, K. Jagannadha Rao, E. S. Choi, B. H. Chung, C. H. Kim, and S. K. Rhee (2001) Current status of the anticoagulant hirudin: Its biotechnological production and clinical practice. Appl. Microbiol. Biotechnol. 57: 606-613.
6. Sohn, J. H., S. K. Lee, E. S. Choi, and S. K. Rhee (1991) Gene expression and secretion of the anticoagulant hirudin in Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 1: 266-273.
7. Khuri, A. I and A. Cornell (1987) Statistics: Text books and monographs, pp. 32-54. In: D. B. Cowen, R. G. Cornell, A. M. Kshirsagar, W. J. Kennedy and E. G. Schilling (eds.). Response Surfaces Designs and Analyses. Marcel Dekker, Inc., New York, USA.
8. Lee, D. H., J. H. Seo, J. H. Sohn, E. S. Choi, and S. K. Rhee (1994) Optimization of environmental conditions for hirudin production from recombinant Saccharomyces cerevisiae. Korean J. Biotechnol. Bioeng. 9: 8-15.
9. Sohn, J. H., E. S. Choi, B. H. Chung, D. J. Youn, J. H. Seo, and S. K. Rhee (1995) Process development for the production of recombinant hirudin in Saccharomyces cerevisiae: from upstream to downstream. Proc. Biochem. 30: 653-660.
10. Jagannadha Rao, K., C. H. Kim, and S. K. Rhee (2000) Statistical optimization of medium for the production of recombinant hirudin from Saccharomyces cerevisiae using response surface methodology. Process Biochem. 35: 639-647.
11. Ko, S. M. (2001) Secretion of Recombinant Human Serum Albumin in Saccharomyces cerevisiae Strain Lacking Galactokinase Encoded by Gal1. M. S. Thesis, Chungnam National University, Daejeon, Korea.
12. Choi, E. S., J. H. Sohn, and S. K. Rhee (1994) Optimization of the expression system using galactose inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 42: 587-594.
13. Jagannadha R. K., C. H. Kim, B. H. Chung, M. K. Kim, and S. K. Rhee (1999) Suppression of proteolytic degradation of recombinant hirudin from Saccharomyces cerevisiae using the oxygen enriched air. Biotechnol. Lett. 21, 391-394
14. Jagannadha R. K., C. H. Kim, B. H. Chung, J. H. Sohn, and S. K. Rhee (1998) Effect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae. Bioprocess Eng. 19: 385-388