An enzymatic strategy for site-specific immobilization of functional proteins using microbial transglutaminase

Enzyme and Microbial Technology

Volumn 35, Issue 6-7, 2004, Pages 613-618

  Tominaga, Jo ^a   Kamiya, Noriho ^a   Doi, Satoshi ^a   Ichinose, Hirofumi ^b   Goto, Masahiro ^a,b  

^a KYUSHU UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Enzymatic protein immobilization; Molecular orientation; Peptide linker; Site specific protein immobilization; Transglutaminase

Indexed keywords

ALKALINE PHOSPHATASE (AP); MICROBIAL TRANSGLUTAMINASE (MTG); PHYSICAL ADSORPTION; SITE-SPECIFIC IMMOBILIZATION;

ADSORPTION; CHEMICAL MODIFICATION; POLYPEPTIDES; PROTEINS; RESINS; SUBSTRATES;

ENZYME IMMOBILIZATION;

ALKALINE PHOSPHATASE; CASEIN; PEPTIDE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE;

ADSORPTION; AMINO TERMINAL SEQUENCE; CHEMICAL MODIFICATION; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME SPECIFICITY; NONHUMAN; PROTEIN FUNCTION; PROTEIN MODIFICATION;

EID: 7544225226     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2004.08.014     Document Type: Conference Paper

References (23)

1. I. Chibata Immobilized enzymes Research and development 1978 Kodansha Tokyo
2. T. Kodadek Protein microarrays: prospects and problems Chem Biol 8 2001 105 115
3. M.B. Soellner, K.A. Dickson, B.L. Nilsson, and R.T. Raines Site-specific protein immobilization by Staudinger ligation J Am Chem Soc 125 2003 11790 11791
4. R. Hahn, E. Berger, K. Pflegerl, and A. Jungbauer Directed immobilization of peptide ligands to accessible pore sites by conjugation with a placeholder molecule Anal Chem 75 2003 543 548
5. M. Nisnevitch, and M.A. Firer The solid phase in affinity chromatography strategies for antibody attachment J Biochem Biophys Methods 49 2001 467 480
6. M.R. Oates, W. Clarke, E.M. Marsh, and D.S. Hage Kinetic studies on the immobilization of antibodies to high-performance liquid chromatographic supports Bioconjugate Chem 9 1998 459 465
7. Z. Bilkova, J. Churacek, Z. Kucerova, and J. Turkova Purification of anti-chymotrypsin antibodies for the prepatation of a bioaffinity matrix with oriented chymotrypsin as immobilized ligand. J Chromatogr B: Biomed Sci Appl 689 1997 273 279
8. Z. Bilkova, J. Mazurova, J. Churacek, D. Horak, and J. Turkova Oriented immobilization of chymotrypsin by use of suitable antibodies coupled to a nonporous solid support. J Chromatogr A 852 1999 141 149
9. D.A. Butterfield, D. Bhattacharyya, S. Daunert, and L. Bachas Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review J Membr Sci 181 2001 29 37
10. W. Huang, J. Wang, D. Bhattacharyya, and L.G. Butterfield Improved enzymatic activity by site-specific immobilization of subtilisin Anal Chem 69 1997 4601 4607
11. S. Vishwanath, J. Wang, D.A. Bachas, D.A. Butterfield, and D. Bhattacharyya Site-directed and random immobilization of subtilisin on functionalized membranes: activity determination in aqueous and organic media Biotechnol Bioeng 60 1998 608 616
12. T. Kanaji, H. Ozaki, T. Takao, H. Kawajiri, H. Ide, and M. Motoki Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112 J Biol Chem 268 1993 11565 11572
13. M. Motoki, and K. Seguro Transglutaminase and its use for food processing Trends Food Sci Technol 9 1998 204 210
14. N. Kamiya, T. Takazawa, T. Tanaka, H. Ueda, and T. Nagamune Site-specific cross-linking of functional proteins by transglutamination Enzyme Microb Technol 30 2002 266 272
15. N. Kamiya, T. Tanaka, T. Suzuki, T. Takazawa, S. Takeda, and K. Watanabe S-peptide as a potent peptidyl linker for protein cross-linking by microbial transglutaminase from Streptomyces mobaraensis Bioconjugate Chem 14 2003 351 357
16. T. Tanaka, N. Kamiya, and T. Nagamune Peptidyl linkers for protein heterodimerization catalyzed by microbial transglutaminase Bioconjugate Chem 15 2004 491 497
17. Y. Kamata, E. Ishikawa, and M. Motoki Enzyme immobilization on ion exchangers by forming an enzyme coating with transglutaminase as a crosslinker Biosci Biotech Biochem 56 1992 1323 1324
18. H. Kawakita, K. Sugita, K. Saito, M. Tamada, T. Sugo, and H. Kawamoto Production of cycloisomaltooligosaccharides from dextran using enzyme immobilized in multilayers onto porous membranes Biotechnol Prog 18 2002 465 469
19. A. Josten, M. Meusel, F. Spener, and L. Haalck Enzyme immobilization via microbial transglutaminase: a method for the generation of stable sensing surface J Mol Catal B: Enzym 7 1999 57 66
20. T. Takazawa, N. Kamiya, H. Ueda, and T. Nagamune Enzymatic labeling of a single chain variable fragment of an antibody with alkaline phosphatase by microbial transglutaminase Biotechnol Bioeng 86 2004 399 404
21. G.A.H. Jong, G. Wijngaards, H. Boumans, S.J. Koppelman, and M. Hessing Purification and substrate specificity of transglutaminase from Blood and Streptoverticillium mobaraense J Agric Food Chem 49 2001 3389 3393
22. E.E. Kim, and W. Wyckoff Reaction mechanism of alkaline phosphatase based on crystal structures J Mol Biol 218 1991 449 461
23. G.T. Hermanson Enzyme modification and conjugation Bioconjugate techniques 1996 Academic Press San Diego, CA [chapter 16]