Site-directed and random immobilization of subtilisin on functionalized membranes: Activity determination in aqueous and organic media

Biotechnology and Bioengineering

Volumn 60, Issue 5, 1998, Pages 608-616

  Viswanath, S ^a,b   Wang, J ^b   Bachas, L G ^b   Butterfield, D A ^b   Bhattacharyya, D ^a,b  

^a UNIVERSITY OF KENTUCKY   (United States)

^b UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Membrane; Oriented immobilization; Site directed mutagenesis; Subtilisin

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; ENZYME KINETICS; HYDROPHOBICITY; MICROFILTRATION; MUTAGENESIS; ORGANIC SOLVENTS; POLYMERIC MEMBRANES; POLYSULFONES;

HYDROPHILICITY; SITE-DIRECTED MUTAGENESIS;

ENZYME IMMOBILIZATION;

MEMBRANE PROTEIN; SUBTILISIN;

ARTICLE; BACILLUS SUBTILIS; ENZYME ACTIVE SITE; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME LOCALIZATION; HYDROPHILICITY; IMMOBILIZATION; MICROFILTRATION; NONHUMAN; SITE DIRECTED MUTAGENESIS;

1-BUTANOL; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HEXANES; KINETICS; MEMBRANES, ARTIFICIAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; ORGANIC CHEMICALS; POLYMERS; SOLVENTS; SUBTILISINS; SULFONES; WATER;

EID: 0032488339     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19981205)60:5<608::AID-BIT11>3.0.CO;2-Q     Document Type: Article

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