Effects of dihydrotestrone on osteoblast gene expression in osteopenic ovariectomized rats

Endocrine Research

Volumn 30, Issue 3, 2004, Pages 361-368

  Davey, Rachel A ^a,b   Morris, H A ^a  

^a HANSON INSTITUTE   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Androgens; Osteoblast genes; Osteopenia; Ovariectomy

Indexed keywords

ALKALINE PHOSPHATASE; ANDROSTANOLONE; COMPLEMENTARY DNA; ESTROGEN; MESSENGER RNA; OSTEOCALCIN; OSTEOPONTIN;

ARTICLE; BODY WEIGHT; BONE TURNOVER; CONTROLLED STUDY; DNA PROBE; DOSE RESPONSE; FEMALE; GENE EXPRESSION; HORMONE ACTION; HORMONE INHIBITION; NONHUMAN; OSSIFICATION; OSTEOBLAST; OSTEOPENIA; OVARIECTOMY; POSTOPERATIVE PERIOD; PRIORITY JOURNAL; RAT; TREATMENT OUTCOME;

ALKALINE PHOSPHATASE; ANDROGENS; ANIMALS; BONE DISEASES, METABOLIC; COLLAGEN TYPE I; DIHYDROTESTOSTERONE; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; FOLLOW-UP STUDIES; GENE EXPRESSION REGULATION; OSTEOBLASTS; OSTEOCALCIN; OSTEOPONTIN; OVARIECTOMY; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; SIALOGLYCOPROTEINS; TESTOSTERONE;

EID: 7544222961     PISSN: 07435800     EISSN: None     Source Type: Journal    
DOI: 10.1081/ERC-200033578     Document Type: Article

References (28)

1. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 1990; 143:420-430.
2. Need AG, Morris HA, Hartley TF, Horowitz M, Nordin BE. Effects of nandrolone decanoate on forearm mineral density and calcium metabolism in osteoporotic postmenopausal women. Calcif Tissue Int 1987; 41:7-10.
3. Johansen JS, Hassager C, Podenphant J, Riis BJ, Hartwell D, Thomsen K, Christiansen C. Treatment of postmenopausal osteoporosis: is the anabolic steroid nandrolone decanoate a candidate? Bone Miner 1989; 6:77-86.
4. Tobias JH, Gallagher A, Chambers TJ. 5 alpha-Dihydrotestosterone partially restores cancellous bone volume in osteopenic ovariectomized rats. Am J Physiol 1994; 267:E853-E859.
5. Coxam V, Bowman BM, Mecham M, Roth CM, Miller MA, Miller SC. Effects of dihydrotestosterone alone and combined with estrogen on bone mineral density, bone growth, and formation rates in ovariectomized rats. Bone 1996; 19:107-114.
6. Colvard DS, Eriksen EF, Keeling PE, Wilson EM, Lubahn DB, French FS, Riggs BL, Spelsberg TC. Identification of androgen receptors in normal human osteoblast-like cells. Proc Natl Acad Sci U S A 1989; 86:854-857.
7. Masuyama A, Ouchi Y, Sato F, Hosoi T, Nakamura T, Orimo H. Characteristics of steroid hormone receptors in cultured MC3T3-E1 osteoblastic cells and effect of steroid hormones on cell proliferation. Calcif Tissue Int 1992; 51:376-381.
8. Goulding A, Gold E. Flutamide-mediated androgen blockade evokes osteopenia in the female rat. J Bone Miner Res 1993; 8:763-769.
9. Durbridge TC, Morris HA, Parsons AM, Parkinson IH, Moore RJ, Porter S, Need AG, Nordin BE, Vernon-Roberts B. Progressive cancellous bone loss in rats after adrenalectomy and oophorectomy. Calcif Tissue Int 1990; 47:383-387.
10. Somjen D, Weisman Y, Mor Z, Harell A, Kaye AM. Regulation of proliferation of rat cartilage and bone by sex steroid hormones. J Steroid Biochem Mol Biol 1991; 40:717-723.
11. Gray C, Colston KW, Mackay AG, Taylor ML, Arnett TR. Interaction of androgen and 1,25-dihydroxyvitamin D3: effects on normal rat bone cells. J Bone Miner Res 1992; 7:41-46.
12. Kasperk CH, Wergedal JE, Parley JR, Linkhart TA, Turner RT, Baylink DJ. Androgens directly stimulate proliferation of bone cells in vitro. Endocrinology 1989; 124:1576-1578.
13. Davey RA, Hahn CN, May BK, Morris HA. Osteoblast gene expression in rat long bones: effects of ovariectomy and dihydrotestosterone on mRNA levels. Calcif Tissue Int 2000; 67:75-79.
14. Wronski TJ, Walsh CC, Ignaszewski LA. Histologic evidence for osteopenia and increased bone turnover in ovariectomized rats. Bone 1986; 7:119-123.
15. Curran T, Gordon MB, Rubino KL, Sambucetti LC. Isolation and characterization of the c-fos(rat) cDNA and analysis of post-translational modification in vitro. Oncogene 1987; 2:79-84.
16. Genovese C, Rowe D, Kream B. Construction of DNA sequences complementary to rat alpha 1 and alpha 2 collagen mRNA and their use in studying the regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D. Biochemistry 1984; 23(25):6210-6216.
17. Thiede MA, Yoon K, Golub EE, Noda M, Rodan GA. Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene. Proc Natl Acad Sci U S A 1988; 85:319-323.
18. Yoon K, Buenaga R, Rodan GA. Tissue specificity and developmental expression of rat osteopontin. Biochem Biophys Res Commun 1987; 148:1129-1136.
19. Yoon KG, Rutledge SJ, Buenaga RF, Rodan GA. Characterization of the rat osteocalcin gene: stimulation of promoter activity by 1,25-dihydroxyvitamin D3. Biochemistry 1988; 27(23):8521-8526.
20. Tso JY, Sun XH, Kao TH, Reece KS, Wu R. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res 1985; 13:2485-2502.
21. Mason RA, Morris HA. Effects of dihydrotestosterone on bone biochemical markers in sham and oophorectomized rats. J Bone Miner Res 1997; 12:1431-1437.
22. Wronski TJ, Dann LM, Scott KS, Cintron M. Long-term effects of ovariectomy and aging on the rat skeleton. Calcif Tissue Int 1989; 45:360-366.
23. Baldock PA, Need AG, Moore RJ, Durbridge TC, Morris HA. Discordance between bone turnover and bone loss: effects of aging and ovariectomy in the rat. J Bone Miner Res 1999; 14:1442-1448.
24. Hofbauer LC, Hicok KC, Khosla S. Effects of gonadal and adrenal androgens in a novel androgen-responsive human osteoblastic cell line. J Cell Biochem 1998; 71:96-108.
25. Kasperk CH, Faehling K, Borcsok I, Ziegler R. Effects of androgens on subpopulations of the human osteosarcoma cell line SaOS2. Calcif Tissue Int 1996; 58:376-382.
26. Vaishnav R, Beresford JN, Gallagher JA, Russell RG. Effects of the anabolic steroid stanozolol on cells derived from human bone. Clin Sci (Lond) 1988; 74:455-460.
27. Kasperk CH, Wakley GK, Hierl T, Ziegler R. Gonadal and adrenal androgens are potent regulators of human bone cell metabolism in vitro. J Bone Miner Res 1997; 12:464-471.
28. Katzburg S, Lieberherr M, Ornoy A, Klein BY, Hendel D, Somjen D. Isolation and hormonal responsiveness of primary cultures of human bone-derived cells: gender and age differences. Bone 1999; 25:667-673.