Isolation and hormonal responsiveness of primary cultures of human bone-derived cells: Gender and age differences

Bone

Volumn 25, Issue 6, 1999, Pages 667-673

  Katzburg, S ^a   Lieberherr, M ^b   Ornoy, A ^a   Klein, B Y ^c   Hendel, D ^d   Somjen, D ^e  

^a HEBREW UNIVERSITY   (Israel)

^b CNRS   (France)

^c HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

^d SHAARE ZEDEK MEDICAL CENTER   (Israel)

^e TEL AVIV SOURASKY MEDICAL CENTER   (Israel)

Author keywords

Gender and age specificity; Hormonal responsiveness; Human trabecular bone; Primary osteoblast cultures

Indexed keywords

ALKALINE PHOSPHATASE; ANDROSTANOLONE; CALCITRIOL; CYCLIC AMP; ESTRADIOL; OSTEOCALCIN; PARATHYROID HORMONE; PROGESTERONE;

ADULT; AGE; AGED; ARTICLE; CELL CULTURE; CELL GROWTH; COLLAGEN SYNTHESIS; CONTROLLED STUDY; FEMALE; HORMONE RESPONSE; HUMAN; HUMAN CELL; MALE; OSTEOBLAST; SEX DIFFERENCE; TRABECULAR BONE;

ALKALINE PHOSPHATASE; CALCIFICATION, PHYSIOLOGIC; CALCITRIOL; CELL AGING; CELL CULTURE TECHNIQUES; CELL DIVISION; COLLAGEN; CYCLIC AMP; ESTRADIOL; FEMALE; HORMONES; HUMANS; MALE; MIDDLE AGED; OSTEOBLASTS; OSTEOCALCIN; PARATHYROID HORMONE; SEX CHARACTERISTICS;

EID: 0032741447     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(99)00225-2     Document Type: Article

References (50)

1. Aronow M.A., Gerstenfeld L.C., Owen T.A., Tassinari M.S., Stein G.S., Lian J.B. Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells. J Cell Physiol. 143:1990;213-221.
2. Aubin J.A., Liu F. The osteoblast lineage. Bilezikian J.P., Raisz L.G., Rodan G. Principles of Bone Biology. 1996;51-67 Academic, San Diego, CA.
3. Aubin J.A., Turksen K., Heersche J.N.M. Osteoblastic cell lineage. Noda M. Cellular and Molecular Biology of Bone. 1993;1-45 Academic, San Diego, CA.
4. Bauer P.J. Affinity and stoichiometry of calcium binding by Arsenzano III. Anal Biochem. 10:1981;61-72.
5. Beresford J.N., Gallagher J.A., Gowen M., McGuire M.K.B., Poser J.W., Russel R.G.G. Human bone cells in culture. A novel system for the investigation of bone cell metabolism. Clin Sci. 64:1983;38-39.
6. 3, parathyroid hormone and glucocorticoids. Metab Bone Dis Rel Res. 5:1984;229-234.
7. 3 and human bone-derived cells in vitro Effects on alkaline phosphatase, type I collagen and proliferation . Endocrinology. 119:1986;1776-1785.
8. Beresford J.N., Graves S.E., Smoothy C.A. Formation of mineralized nodules by bone derived cells in vitro A model of bone formation? Am J Med Genet. 45:1993;163-178.
9. 3 on osteogenic differentiation of human marrow stromal cells in vitro. Arch Oral Biol. 39:1994;941-947.
10. Binderman I., Berger E., Fine N., Shimshoni Z., Harell A., Somjen D. Calvaria derived osteogenic cells Phenotypic expression in culture . Connect Tissue Res. 20:1989;41-49.
11. Bouillon R., Bex M., Van Herck E., Laureys J., Doomes L., Lesaffre E., Ravussin E. Influence of age, sex, and insulin on osteoblast function Osteoblast dysfunction in diabetes mellitus . J Clin Endocrinol Metab. 80:1995;1194-1202.
12. Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
13. Bronckers A.L.J.J., Gay S., Finkelman R.D., Butler W.T. Developmental appearance of the Gla proteins (osteocalcin) and alkaline phosphatase in tooth germs and bone of the rat. Bone Miner Res. 2:1987;361-373.
14. Chavassieux P.M., Chenu C., Valentin-Opran A., Merle B., Delmas P.D., Hartmann D.J., Saez S., Meunier P.J. Influence of experimental conditions on osteoblast activity in human primary bone cell cultures. J Bone Miner Res. 5:1990;337-343.
15. Farley J.R., Ivey J.L., Baylink D.J. Human skeletal alkaline phosphates. J Biol Chem. 255:1980;4680-4686.
16. Fleet J.C., Hock J.M. Identification of osteocalcin mRNA in non-osteoid tissue of rats and humans by reverse transcription-polymerase chain reaction. J Bone Miner Res. 9:1994;1565-1573.
17. Fournier B., Price P. Characterization of a new human osteosarcoma cell line OHS-4. J Cell Biol. 114:1991;577-583.
18. Gehron Robey P. Collagenase-treated trabecular bone fragments A reproducible source of cells in the osteoblastic lineage . Calcif Tissue Int. 56:(Suppl. 1):1995;S11-S12.
19. Gehron Robey, P. Model systems to study osteoblast differentiation. In: The Annual Meeting of the Endocrinology Society, New Orleans, LA; 1998; Abstract.
20. Gehron Robey P., Termine J.D. Human bone cells in vitro. Calcif Tissue Int. 37:1985;453-460.
21. Goldman R., Bar-Sahvit Z. Dual effects of normal and stimulated macrophages and their conditioned media on target cell proliferation. J National Cancer Inst. 63:1979;1009-1016.
22. Hock J.M., Onyia J., Bidwell J. Comparisons of in vivo and in vitro models of the response of osteoblasts to hormonal regulation with aging. Calcif Tissue Int. 56:(Suppl. 1):1995;S44-S47.
23. 3 on the function, cytochemistry and morphology of normal human osteoblast-like cells. Differentiation. 55:1994;153-163.
24. Jonsson, B. K., Frost, A., Ljunghall, S., and Ljunggren, O. Three isolation techniques for primary culture of human osteoblast-like cells: Comparison. In: The Annual Meeting of the Endocrinology Society, New Orleans, LA; 1998; Abstract.
25. Klein, B., Gal, I., and Segal, D. Studies of the levamisole inhibitory effect on rat stromal-cell commitment to mineralization. J Cell Biochem 53:114-112; 1993.
26. Klein B.Y., Rojansky N., Gal I., Shlomi Z., Liebergall M., Ben-Bassat H. Analysis of cell-mediated mineralization in culture of bone-derived embryonic cells with neurofibromatosis. J Cell Biochem. 57:1995;530-542.
27. Kudo Y., Itatsu S., Iwashita M., Iguchi T., Takeda Y. Effects of estrogen and parathyroid hormone on osteoblastic activity via regulating the binding activity of insulin-like growth factor binding protein-4 in SaOS-2 cells Implications for the pathogenesis of postmenopausal osteoporois . Biochim Biophys Acta. 1245:1995;402-406.
28. Kung Sutherland M.S., Rao L.G., Muzaffar S.A., Wylie J.N., Wong M.M., McBroom R.J., Murray T.M. Age-dependent expression of osteoblastic phenotypic markers in normal human osteoblasts cultured long term in the presence of dexamethasone. Osteopor Int. 5:1995;335-343.
29. Liu F., Malaval L., Gupta A.K., Aubin J.E. Simultaneous detection of multiple bone-related mRNAs and protein expression during osteoblast differentiation Polymerase chain reaction and immunocytochemical studies at the single cell level . Dev Biol. 166:1994;220-234.
30. 2 in normal and postmenopausal osteoporotic women. J Bone Miner Res. 5:1990;1149-1155.
31. Majeska R.J., Rodan S.B., Rodan G.A. Parathyroid hormone-responsive clonal cell lines from rat osteosarcoma. Endocrinology. 107:1980;1494-1503.
32. Marie P.J., Sabbagh A., de Vernejoul M.C., Lomri A. Osteocalcin and deoxyribonucleic acid synthesis in vitro and histomorphometric indices of bone formation in postmenopausal osteoporosis. J Clin Endocrinol Metab. 69:1989;272-279.
33. Mark M.P., Butler W.T., Prince C.W., Finkelman R.D., Ruch J.-V. Developmental expression of 44 kDa bone phosphoprotein (osteopontin) and bone γ-carboxyglutamic acid (Gla)-containing protein (osteocalcin) in calcified tissues of rat. Differentiation. 37:1988;123-136.
34. Owen T.A., Aronow M.S., Barone L.M., Bettencourt B., Stein G.S., Lian J.B. Pleiotropic effects of vitamin D on osteoblast gene expression are related to the proliferative and differentiated state of the bone cell phenotype Dependency upon basal levels of gene expression, duration of exposure, and bone matrix competency in normal rat osteoblast cultures . Endocrinology. 128:1991;1496-1504.
35. Parfitt A.M., Shih M.-S., Rao D.S., Kleerekoper M. Relation between bone formation rate and osteoblast surface in aging and osteoporosis Evidence for impaired osteoblast recruitment in pathogenesis . J Bone Miner Res. 7:(Suppl. 1):1991;S116.
36. Pavlin D., Bedalov A., Kronenberg M.S., Kream B.E., Rowe D.W., Smith C.L., Pike J.W., Lichtler A.C. Analysis of regulatory regions in the COL1A1 gene responsible for 1,25-dihydroxyvitamin D3-mediated transcriptional repression in osteoblastic cells. J Cell Biochem. 56:1994;490-501.
37. Peck W.A., Birge S.J., Fedak S.A. Bone cells Biochemical and biological studies after enzymatic isolation . Science. 146:1964;1476-1477.
38. Peterkovsky B., Diegelman R. Use of mixture of proteinase free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 10:1971;988-993.
39. 3 increases synthesis of the vitamin K-dependent bone protein by osteosarcoma cells. J Biol Chem. 255:1989;11660-11663.
40. Price P.A., Parthemore J.G., Deftos L.J. New biochemical marker for bone metabolism. Measurement by radioimmunoassay of bone gla protein in the plasma of normal subjects and patients with bone disease. J Clin Invest. 66:1980;878-883.
41. Raisz L.G., Maina D.M., Gworek S.G., Dietrick J.W., Canalis E.M. Hormonal control of bone collagen synthesis in vitro Inhibitory effect of 1-hydroxylated vitamin D metabolites . Endocrinology. 102:1978;731-735.
42. Rodan G.A., Heath J.K., Yoon K., Noda M., Rodan S.B. Diversity of the osteoblastic phenotype. Evered D., Harnett S. Cell and Molecular Biology of Vertebrate Hard Tissues. 1988;78-91 Wiley, Chichester, UK.
43. Rodan G.A., Martin T.J. Role of osteoblasts in hormonal control of bone resorption A hypothesis . Calcif Tissue Int. 33:1981;349-351.
44. Rodan G.A., Rodan S.B. Expression of the osteoblastic phenotype. Peck W.A. Bone and Mineral Research Annual 2. 1984;244-285 Elsevier, Amsterdam.
45. Spelsberg T.C., Harris S.A., Riggs B.L. Immortalized osteoblast cell systems (new human fetal osteoblast systems). Calcif Tissue Int. 56:(Suppl. 1):1995;S18-S21.
46. Thiede M.A., Smock S.L., Petersen D.N., Grasser W.A., Thompson D.D., Nishimoto S.K. Presence of messenger ribonucleic acid encoding osteocalcin, a marker of bone turnover, in bone marrow megakaryocytes and peripheral blood platelets. Endocrinology. 135:1994;929-937.
47. Turner R.T., Colvard D.S., Spelberg T.C. Estrogen inhibition of periosteal bone formation in rat long bones Down-regulation of gene expression for bone matrix proteins . Endocrinology. 127:1990;1346-1351.
48. 3-analogs on osteoblast-like cells and on in vitro bone resorption. J Ster Biochem Mol Biol. 55:1995;337-346.
49. Wlodarsky K.H., Reddi A.H. Alkaline phosphatase as a marker of osteoinductive cells. Calcif Tissue Int. 39:1986;382-385.
50. Wuthier R.E., Register T.C. Role of alkaline phosphatase, a polyfunctional enzyme, in mineralized tissues. Butler W.T. The Chemistry and Biology of Mineralized Tissues. 1984;113-124 Ebsco, Birmingham, UK.