High-level expression of lipase in Escherichia coli and recovery of active recombinant enzyme through in vitro refolding

Protein Expression and Purification

Volumn 70, Issue 1, 2010, Pages 75-80

  Akbari, Neda ^a   Khajeh, Khosro ^b   Rezaie, Sasan ^c   Mirdamadi, Saeed ^d   Shavandi, Mahmoud ^e   Ghaemi, Nasser ^a,f  

^a ISLAMIC AZAD UNIVERSITY   (Iran)

^b TARBIAT MODARES UNIVERSITY   (Iran)

^c TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^d IRANIAN RESEARCH ORGANIZATION FOR SCIENCE AND TECHNOLOGY IROST   (Iran)

^e RESEARCH INSTITUTE OF PETROLEUM INDUSTRY RIPI   (Iran)

^f UNIVERSITY OF TEHRAN   (Iran)

Author keywords

Lipase; Lipase specific foldase; Overexpression; Refolding

Indexed keywords

BACTERIAL PROTEIN; HYBRID PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; CELL INCLUSION; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN FOLDING; PSEUDOMONAS;

BACTERIAL PROTEINS; ESCHERICHIA COLI; INCLUSION BODIES; LIPASE; PROTEIN FOLDING; PSEUDOMONAS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 75349112052     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.08.009     Document Type: Article

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