Overproduction in Escherichia coli, purification and characterization of a family I.3 lipase from Pseudomonas sp. MIS38

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1478, Issue 2, 2000, Pages 201-210

  Amada, Kei ^a   Haruki, Mitsuru ^a   Imanaka, Tadayuki ^b   Morikawa, Masaaki ^a   Kanaya, Shigenori ^a  

^a OSAKA UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Ca2+ binding; Lipase; Pseudomonas; Recombinant protein; Refolding; Site directed mutagenesis

Indexed keywords

MUTANT PROTEIN; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ARTICLE; CALCIUM BINDING; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; MOLECULAR WEIGHT; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FOLDING; PSEUDOMONAS; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CALCIUM; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; LIPASE; MOLECULAR SEQUENCE DATA; MUTATION; PSEUDOMONAS; SEQUENCE ALIGNMENT; TEMPERATURE;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 0034704990     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00046-7     Document Type: Article

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