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Volumn 81, Issue 1, 2010, Pages

Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEX MIXTURE; DNA HYBRIDIZATION; DNA MICRO-ARRAY; EQUILIBRIUM ISOTHERMS; KINETIC MODELS; MODEL PREDICTION; SLOW RELAXATIONS; SYSTEMATIC DEVIATION; TARGET SEQUENCES; THERMODYNAMIC EQUILIBRIA;

EID: 75349100268     PISSN: 15393755     EISSN: 15502376     Source Type: Journal    
DOI: 10.1103/PhysRevE.81.012901     Document Type: Article
Times cited : (23)

References (24)
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    • We note that the slope in the nonequilibrium regime of Fig. is somewhat smaller than that of Figs. This is probably due to the protocol followed: at time t=0 both the solution containing the target molecules and the array are at room temperature. They are then placed into an oven for the duration of the experiment at a temperature T=65°C. Short time behavior could then be influenced by the initial room-temperature hybridization.
    • We note that the slope in the nonequilibrium regime of Fig. is somewhat smaller than that of Figs. This is probably due to the protocol followed: at time t=0 both the solution containing the target molecules and the array are at room temperature. They are then placed into an oven for the duration of the experiment at a temperature T=65°C. Short time behavior could then be influenced by the initial room-temperature hybridization.
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    • We estimated Δ GPM (L=30) =-14.5 kcal/mol from the fits of Fig., using extensivity we expect Δ GPM (L=25) 0.83Δ GPM (L=30) =-12 kcal/mol.
    • We estimated Δ GPM (L=30) =-14.5 kcal/mol from the fits of Fig., using extensivity we expect Δ GPM (L=25) 0.83Δ GPM (L=30) =-12 kcal/mol.


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