Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis

Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

Volumn 151, Issue 3, 2010, Pages 325-333

  Fang, Yan ^a,b   Yang, Hongsheng ^a   Wang, Tianming ^a,b   Liu, Baozhong ^a   Zhao, Heling ^a,b   Chen, Muyan ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Cadmium; Mactra veneriformis; Metallothionein; mRNA expression; Superoxide dismutase

Indexed keywords

CADMIUM; COMPLEMENTARY DNA; COPPER; MESSENGER RNA; METALLOTHIONEIN; NUCLEOTIDE; SUPEROXIDE DISMUTASE; ZINC;

AMINO ACID SEQUENCE; AQUATIC ENVIRONMENT; ARTICLE; CELL METABOLISM; CLAM; CONTROLLED STUDY; ENZYME ACTIVITY; EXOCRINE GLAND; EXPOSURE; HOMEOSTASIS; LETHALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CONTENT; RNA TRANSCRIPTION; SEQUENCE HOMOLOGY; STRESS; WATER POLLUTION;

BIVALVIA; MACTRA VENERIFORMIS; MOLLUSCA;

EID: 75349095570     PISSN: 15320456     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpc.2009.12.005     Document Type: Article

References (68)

1. Almeida E.A., Miyamoto S., Bainy A.C.D., Medeiros M.H.G., and Mascio P.D. Protective effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals. Mar. Pollut. Bull. 49 (2004) 386-392
2. Amiard J.C., Amiard-Triquet C., Barka S., Pellerin J., and Rainbow P.S. Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers. Aquat. Toxicol. 76 (2006) 160-202
3. Baudrimont M., Metivaud J., MauryBrachet R., Ribeyre F., and Boudou A. Bioaccumulation and metallothionein response in the Asiatic clam (Corbicula fluminea) after experimental exposure to cadmium and inorganic mercury. Environ. Toxicol. Chem. 16 (1997) 2096-2105
4. Benavides M.P., Gallego S.M., and Tomaro M.L. Cadmium toxicity in plants. Braz. J. Plant Physiol. 17 (2005) 21-34
5. Bertin G., and Averbeck D. Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review). Biochimie 88 (2006) 1549-1559
6. Blaschke V., Reich K., Blaschke S., Zipprich S., and Neumann C. Rapid quantitation of proinflammatory and chemoattractant cytokine expression in small tissue samples and monocyte-derived dendritic cells: validation of a new real-time RT-PCR technology. J. Immunol. Methods 246 (2000) 79-90
7. Boutet I., Tanguy A., and Moraga D. Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions. Gene 329 (2004) 147-157
8. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of the protein-dye binding. Anal. Biochem. 72 (1976) 248-254
9. Cai L., Satoh M., Tohyama C., and Cherian M.G. Metallothionein in radiation exposure: its induction and protective role. Toxicology 132 (1999) 85-98
10. Canesi L., Lorusso L.C., Ciacci C., Betti M., Rocchi M., Pojana G., and Marcomini A. Immunomodulation of Mytilus hemocytes by individual estrogenic chemicals and environmentally relevant mixtures of estrogens: in vitro and in vivo studies. Aquat. Toxicol. 81 (2007) 36-44
11. Cantú-Medellín N., Olguín-Monroy N.O., Méndez-Rodríguez L.C., and Zenteno-Savín T. Antioxidant enzymes and heavy metal levels in tissues of the black chocolate clam Megapitaria squalida in Bahia de La Paz, Mexico. Arch. Environ. Contam. Toxicol. 56 (2009) 60-66
12. Cavaletto M., Ghezzi A., Burlando B., Evangelisti V., Ceratto N., and Viarengo A. Effect of hydrogen peroxide on antioxidant enzymes and metallothionein level in the digestive gland of Mytilus galloprovincialis. Comp. Biochem. Physiol. C 131 (2002) 447-455
13. Chandran R., Sivakumar A.A., Mohandass S., and Aruchami M. Effect of cadmium and zinc on antioxidant enzyme activity in the gastropod, Achatina fulica. Comp. Biochem. Physiol. C 140 (2005) 422-426
14. Chang Y.T., Jong K.J., Liao B.K., and Wu S.M. Cloning and expression of metallothionein cDNA in the hard clam (Meretrix lusoria) upon cadmium exposure. Aquaculture 262 (2007) 504-513
15. Chelomin V.P., Zakhartsev M.V., Kurilenko A.V., and Belcheva N.N. An in vitro study of the effect of reactive oxygen species on subcellular distribution of deposited cadmium in digestive gland of mussel Crenomytilus grayanus. Aquat. Toxicol. 73 (2005) 181-189
16. Cho Y.S., Choi B.N., Kim K.H., Kim S.K., Kim D.S., Bang I.C., and Nam Y.K. Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture 253 (2006) 667-679
17. Choi C.Y., An K.W., Nelson E.R., and Habibi H.R. Cadmium affects the expression of metallothionein (MT) and glutathione peroxidase (GPX) mRNA in goldfish, Carassius auratus. Comp. Biochem. Physiol. C 145 (2007) 595-600
18. Choi Y.K., Jo P.G., and Choi C.Y. Cadmium affects the expression of heat shock protein 90 and metallothionein mRNA in the Pacific oyster, Crassostrea gigas. Comp. Biochem. Physiol. C 147 (2008) 286-292
19. Company R., Serafim A., Cosson R., Camus L., Shillito B., Fiala-Medioni A., and Bebianno M.J. The effect of cadmium on antioxidant responses and the susceptibility to oxidative stress in the hydrothermal vent mussel Bathymodiolus azoricus. Mar. Biol. 148 (2006) 817-825
20. Corticeiro S.C., Lima A.I.G., and Figueira E. The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure. Enzyme Microb. Technol. 40 (2006) 132-137
21. Dalton T., Palmiter R.D., and Andrews G.K. Transcriptional induction of the mouse metallothionein-i gene in hydrogen peroxide-treated hepa cells involves a composite major late transcription factor antioxidant response element and metal response promoter elements. Nucl. Acids Res. 22 (1994) 5016-5023
22. Dondero F., Piacentini L., Banni M., Rebelo M., Burlando B., and Viarengo A. Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation. Gene 345 (2005) 259-270
23. Faria M., Carrasco L., Diez S., Riva M.C., Bayona J.M., and Barata C. Multi-biomarker responses in the freshwater mussel Dreissena polymorpha exposed to polychlorobiphenyls and metals. Comp. Biochem. Physiol. C 149 (2009) 281-288
24. Filion M., St-Arnaud M., and Jabaji-Hare S.H. Direct quantification of fungal DNA from soil substrate using real-time PCR. J. Microbiol. Methods 53 (2003) 67-76
25. Frenzilli G., Bocchetti R., Pagliarecci M., Nigro M., Annarumma F., Scarcelli V., Fattorini D., and Regoli F. Time-course evaluation of ROS-mediated toxicity in mussels, Mytilus galloprovincialis, during a field translocation experiment. Mar. Environ. Res. 58 (2004) 609-613
26. Fridovich I. Superoxide dismutases. Annu. Rev. Biochem. 44 (1975) 147-159
27. Geret F., and Cosson R.P. Induction of specific isoforms of metallothionein in mussel tissues after exposure to cadmium or mercury. Arch. Environ. Contam. Toxicol. 42 (2002) 36-42
28. Geret F., Jouan A., Turpin V., Bebianno M.J., and Cosson R.P. Influence of metal exposure on metallothionein synthesis and lipid peroxidation in two bivalve mollusks: the oyster (Crassostrea gigas) and the mussel (Mytilus edulis). Aquat. Living Resour. 15 (2002) 61-66
29. Geret F., Serafim A., Barreira L., and Bebianno M.J. Effect of cadmium on antioxidant enzyme activities and lipid peroxidation in the gills of the clam Ruditapes decussatus. Biomarkers 7 (2002) 242-256
30. Halliwell B., and Gutteridge J.M.C. The importance of free-radicals and catalytic metal-ions in human-diseases. Mol. Aspects Med. 8 (1985) 89-193
31. Hansen B.H., Romma S., Garmo O.A., Olsvik P.A., and Andersen R.A. Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. Comp. Biochem. Physiol. C 143 (2006) 263-274
32. Isani G., Andreani G., Cocchioni F., Fedeli D., Carpene E., and Falcioni G. Cadmium accumulation and biochemical responses in Sparus aurata following sub-lethal Cd exposure. Ecotoxicol. Environ. Saf. 72 (2009) 224-230
33. Ivanina A.V., Cherkasov A.S., and Sokolova I.M. Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin. J. Exp. Biol. 211 (2008) 577-586
34. Jenny M.J., Warr G.W., Ringwood A.H., Baltzegar D.A., and Chapman R.W. Regulation of metallothionein genes in the American oyster (Crassostrea virginica): ontogeny and differential expression in response to different stressors. Gene 379 (2006) 156-165
35. Jo P.G., Choi Y.K., and Choi C.Y. Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure. Comp. Biochem. Physiol. C 147 (2008) 460-469
36. Johnston E.L., and Roberts D.A. Contaminants reduce the richness and evenness of marine communities: a review and meta-analysis. Environ. Pollut. 157 (2009) 1745-1752
37. Kobayashi J. Relation between Itai-Itai disease and the pollution of river water by cadmium from mine. In: Jenkins S.H. (Ed). Advances in Water Pollution Research vol. 1 (1971), Pergamon Press, Oxford 1-25
38. Lecoeur S., Videmann B., and Berny P. Evaluation of metallothionein as a biomarker of single and combined Cd/Cu exposure in Dreissena polymorpha. Environ. Res. 94 (2004) 184-191
39. Leignel V., and Laulier M. Isolation and characterization of Mytilus edulis metallothionein genes. Comp. Biochem. Physiol. C 142 (2006) 12-18
40. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using realtime quantitative PCR and the 2(- Delta Delta C(T)) method. Methods 25 (2001) 402-408
41. Margoshes M., and Vallee B.L. A cadmium protein from equine kidney cortex. J. Am. Chem. Soc. 79 (1957) 4813-4814
42. Marigómez I., Soto M., Cajaraville M.P., Angulo E., and Giamberini L. Cellular and subcellular distribution of metals in molluscs. Micros. Res. Technol. 56 (2002) 358-392
43. Matozzo V., Ballarin L., Pampanin D.M., and Marin M.G. Effects of copper and cadmium exposure on functional responses of hemocytes in the clam, Tapes philippinarum. Arch. Environ. Contam. Toxicol. 41 (2001) 163-170
44. Meng W., Qin Y.W., Zheng B.H., and Zhang L. Heavy metal pollution in Tianjin Bohai Bay, China. J. Environ. Sci. 20 (2008) 814-819
45. Mizuno Y. Superoxide dismutase activity in early stages of development in normal and dystrophic chickens. Life Sci. 34 (1984) 909-914
46. Moore M.N., and Icarus Allen J. A computational model of the digestive gland epithelial cell of marine mussels and its simulated responses to oil-derived aromatic hydrocarbons. Mar. Environ. Res. 54 (2002) 579-584
47. Ni D.J., Song L.S., Gao Q., Wu L.T., Yu Y.D., Zhao J.M., Qiu L.M., Zhang H., and Shi F.F. The cDNA cloning and mRNA expression of cytoplasmic Cu, Zn superoxide dismutase (SOD) gene in scallop Chlamys farreri. Fish Shellfish Immunol. 23 (2007) 1032-1042
48. Noor R., Mittal S., and Iqbal J. Superoxide dismutase-applications and relevance to human diseases. Med. Sci. Monit. 8 (2002) RA210-RA215
49. Novelli J., Novelli E.L.B., Manzano M.A., Lopes A.M., Cataneo A.C., Barbosa L.L., and Ribas B.O. Effect of alpha-tocopherol on superoxide radical and toxicity of cadmium exposure. Int. J. Environ. Health Res. 10 (2000) 125-134
50. Olsson P.E., Larsson A., Maage A., Haux C., Bonham K., Zafarullah M., and Gedamu L. Induction of metallothionein synthesis in rainbow-trout, Salmo gairdneri, during long-term exposure to waterborne cadmium. Fish Physiol. Biochem. 6 (1989) 221-229
51. Oyanagui Y. Reevaluation of assay-methods and establishment of kit for superoxide dismutase activity. Anal. Biochem. 142 (1984) 290-296
52. Prusky A.M., and Dixon D.R. Effects of cadmium on nuclear integrity and DNA repair efficiency in the gill cells of Mytilus edulis L. Aquat. Toxicol. 57 (2002) 127-137
53. Rainbow P.S. Trace metal concentrations in aquatic invertebrates: why and so what?. Environ. Pollut. 120 (2002) 497-507
54. Rainbow P.S., and White S.L. Comparative strategies of heavy metal accumulation of Zn, Cu and Cd by crabs and brancles. Estuar. Coast. Shelf Sci. 21 (1989) 669-686
55. Roesijadi G. Metallothionein and its role in toxic metal regulation. Comp. Biochem. Physiol. C 113 (1996) 117-123
56. Roesijadi G., Brubacher L.L., Unger M.E., and Anderson R.S. Metallothionein mRNA induction and generation of reactive oxygen species in molluscan hemocytes exposed to cadmium in vitro. Comp. Biochem. Physiol. C 118 (1997) 171-176
57. Scheuhammer A.M., and Cherian M.G. Quantification of metallothioneins by a silver-saturation method. Toxicol. Appl. Pharmacol. 82 (1986) 417-425
58. Sun Y., Oberley L.W., and Li Y. A simple method for clinical assay of superoxide-dismutase. Clin. Chem. 34 (1988) 497-500
59. Thornalley P.J., and Vasak M. Possible role for metallothionein in protection against radiation-induced oxidative stress-kinetics and mechanism of its reaction with superoxide and hydroxyl radicals. Biochim. Biophys. Acta 827 (1985) 36-44
60. Viarengo A. Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level. CRC Reviews in Aquatic Sciences (1989), CRC Press, Boca Raton, FL, USA 295-317
61. Viarengo A., and Nott J.A. Mechanisms of heavy-metal cation homeostasis in marine-invertebrates. Comp. Biochem. Physiol. C 104 (1993) 355-372
62. Viarengo A., Burlando B., Ceratto N., and Panfoli I. Antioxidant role of metallothioneins: a comparative overview. Cell. Mol. Biol. 46 (2000) 407-417
63. Wang, Z.S., 2007. Study of copper toxicity to a saltwater cladoceram Moina monogolica Daday and its methods of toxicity evaluation. Philosopher Doctor Dissertation, Shanghai Jiao Tong University, Shanghai, China (in Chinese with English abstract).
64. Wang Y.W., Liang L., Shi J.B., and Jiang G.B. Study on the contamination of heavy metals and their correlations in mollusks collected from coastal sites along the Chinese Bohai Sea. Environ. Int. 31 (2005) 1103-1113
65. Wang L.L., Song L.S., Ni D.J., Zhang H., and Liu W.Q. Alteration of metallothionein mRNA in bay scallop Argopecten irradians under cadmium exposure and bacteria challenge. Comp. Biochem. Physiol. C 149 (2009) 50-57
66. 2+ to the clam Mactra veneriformis. Mar. Sci. 12 (2009) 24-39 (in Chinese with English abstract)
67. Xu X.D., Lin Z.H., and Li S.Q. The studied of the heavy metal pollution of Jiaozhou Bay. Mar. Sci. 29 (2005) 48-53 (in Chinese with English abstract)
68. Zorita I., Bilbao E., Schad A., Cancio I., Soto M., and Cajaraville M.P. Tissue- and cell-specific expression of metallothionein genes in cadmium- and copper-exposed mussels analyzed by in situ hybridization and RT-PCR. Toxicol. Appl. Pharmacol. 220 (2007) 186-196