Efficient fusion expression of G13 domain derived from granulysin in Escherichia coli

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 25, Issue 2, 2009, Pages 235-241

  Liu, Xiaoqiang ^a   Zha, Xiangdong ^a   Xiao, Yazhong ^a   Yang, Jinhuan ^a   Li, Nengshu ^a  

^a ANHUI UNIVERSITY   (China)

Author keywords

Cationic antimicrobial peptide; Fusion expression; G13 domain; Granulysin

Indexed keywords

ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

ANTIINFECTIVE AGENT; CYANOGEN BROMIDE; GNLY PROTEIN, HUMAN; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; HYBRID PROTEIN; T LYMPHOCYTE ANTIGEN; THIOREDOXIN;

ARTICLE; BIOSYNTHESIS; CELL INCLUSION; ESCHERICHIA COLI; GENETIC TRANSFECTION; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

ANTI-INFECTIVE AGENTS; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; CYANOGEN BROMIDE; ESCHERICHIA COLI; GTP-BINDING PROTEIN ALPHA SUBUNITS, G12-G13; INCLUSION BODIES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; THIOREDOXINS; TRANSFECTION;

EID: 75349088930     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Guerreiro CI, Fontes CM, Gama M, et al. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expr Purif, 2008, 59(1): 161-168.
2. Bonomo RA. Multiple antibiotic-resistant bacteriain in long-term-care facilities: an emerging problem in the practice of infectious diseases. Clin Infect Dis, 2000, 31(6): 1414-1422.
3. Clayberger C, Krensky AM. Granulysin. Curr Opin Immol, 2003, 15(5): 560-565.
4. Wang Z, Choice E, Kaspar A, et al. Bactericidal and tumoricidal activities of synthetic peptides derived from granulysin. J Immol Lunol, 2000, 165(3): 1486-1490.
5. Xu XX, Jin FL, Yu XQ, et al. High-level expression of the recombinant hybrid peptide cecropinA (1-8)-magainin2 (1-12) with an ubiquitin fusion partner in Escherichia coli. Protein Expr Purif, 2007, 55(1): 175-182.
6. Rodriguez JC, Wong L, Jennings PA. The solvent in CNBr cleavage reactions determines the fragmentation efficiency of ketosteroid isomerase fusion proteins used in the production of recombinant peptides. Protein Expr Purif, 2003, 28(2): 224-231.
7. Li BC, Chen YQ, Liu P, et al. Expression of the antibacterial peptide CM4-Like gene of Chinese silkworm bombyxmori in Escherichia coli and its antibacterial activity analysis. J Mol Cell Biol, 2007, 40(2): 98-101.
8. Stenger S, Hanson DA, Teitelbaum R, et al. An antimicrobial activity of cytolytic T cells mediated by granulysin. Science, 1998, 282(5386): 121-125.
9. Lu HR, Li GD, Wu HY, et al. Fusion expression of the antimicrobial peptide GK1 in Escherichia coli. Chin J Biotech, 2008, 24(1): 21-26.
10. Zha XD, Xu KS, Liu J. Research advances in cationic antimicrobial peptides. Chin Pharmacol Bull, 2003, 19(7): 735-740.
11. Tuo XY, Chai JK. Research progress in gene engineering of antimicrobial peptide. Lett Biotechnol, 2007, 18(2): 298-300.
12. Feng XJ, Wang JH, Shan AS, et al. Fusion expression of bovine lactoferricin in Eschericha coli. Protein Expr Purif, 2006, 47(1): 110-117.
13. Skosyrev VS, Kulesskiy EA, Yakhnim AV, et al. Expression of the recombinant antibacterial peptide sarcotoxin IA in Eschericha coli cells. Protein Expr Purif, 2003, 28(2): 350-356.
14. Hara S, Yamakawa M. Production in Escherichia coli of Moricin, a novel type antibacterial peptide from the silkworm, Bombyx mori. Biochem Biophys Res Commol, 1996, 220(3): 664-669.
15. Li BC, Zhang SQ, Dan WB, et al. Expression in Escherichia coli and purification of bioactive antibacterial peptide ABP-CM4 from the Chinese silk worm, Bombyx mori. Biotechnol Lett, 2007, 29(7): 1031-1036.
16. Li Y, Li X, Wang G. Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies. Protein Expr Purif, 2006, 47(2): 498-505.
17. Yang JH, Zha XD, Fang H, et al. Recombinant expression and toxic effect on the host bacterial viability of G13 domain from granulysin. Chin J Biol, 2008, 21(6): 467-470.
18. Richardsonand JP, Macmillan D. Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation. Org Biomol Chem, 2008, 6: 3977-3982.