Construction of a nontoxigenic Clostridium botulinum strain for food challenge studies

Applied and Environmental Microbiology

Volumn 76, Issue 2, 2010, Pages 387-393

  Bradshaw, Marite ^a   Marshall, Kristin M ^a   Heap, John T ^b   Tepp, William H ^a   Minton, Nigel P ^b   Johnson, Eric A ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BOTULINUM; BOTULINUM NEUROTOXIN TYPE A; BOTULINUM NEUROTOXINS; CLOSTRIDIUM BOTULINUM; FOOD INDUSTRIES; FOOD MATRIXES; GROWTH PROPERTIES; IN-VITRO ASSAYS; LABORATORY ANIMALS; MOUSE BIOASSAY; MUTANT CLONES; NATURAL TOXINS; PARENT STRAIN; PHYSIOLOGICAL CHARACTERISTICS; POTENTIAL THREATS; PROCESSING CONDITION; REGULATORY AGENCIES; SAFETY OF FOOD; TOXIGENIC; WESTERN BLOTS; WILD-TYPE STRAIN;

ANIMALS; CLOSTRIDIUM; LAWS AND LEGISLATION; OCCUPATIONAL RISKS; PERSONNEL TESTING; SAFETY TESTING; STAINLESS STEEL; TOXIC MATERIALS;

BIOASSAY;

BOTULINUM TOXIN;

BACTERIUM; BIOASSAY; BIOSAFETY; FOOD INDUSTRY; TOXIN;

ANIMAL; ARTICLE; CLOSTRIDIUM BOTULINUM; FOOD CONTROL; GENETICS; GROWTH, DEVELOPMENT AND AGING; INTRON; MOUSE; MUTATION; PATHOGENICITY; PULSED FIELD GEL ELECTROPHORESIS;

ANIMALS; BOTULINUM TOXINS; CLOSTRIDIUM BOTULINUM; ELECTROPHORESIS, GEL, PULSED-FIELD; FOOD MICROBIOLOGY; INTRONS; MICE; MUTATION;

ANIMALIA; CLOSTRIDIUM BOTULINUM;

EID: 75249094990     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02005-09     Document Type: Article

References (36)

1. Arnon, S. S., R. Schechter, T. V. Inglesby, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eitzen, A. D. Fine, J. Hauer, M. Layton, S. Lillibridge, M. T. Oslerholm, T. O'Toole, G. Parker, T. M. Perl, P. K. Russell, D. L. Swerdlow, K. Tonat, and the Working Group on Civilian Biodefense. 2001. Botulinum toxin as a biological weapon-medical and public health management. JAMA 285:1059-1070.
2. Bradshaw, M., S. S. Dineen, N. D. Maks, and E. A. Johnson. 2004. Regulation of neurotoxin complex expression in Clostridium botulinum strains 62A, Hall A-hyper, and NCTC 2916. Anaerobe 10:321-333.
3. Busta, F. F., T. V. Suslow, M. E. Parish, L. R. Beuchat, J. N. Farber, E. H. Garrett, and L. J. Harris. 2003. The use of indicators and surrogate microorganisms for the evaluation of pathogens in fresh and fresh-cut produce. Compr. Rev. Food Sci. Food Saf. 2:179-185.
4. Carter, A. T., C. J. Paul, D. R. Mason, S. M. Twine, M. J. Alston, S. M. Logan, J. W. Austin, and M. W. Peck. 2009. Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum. BMC Genomics 10:115.
5. Centers for Disease Control and Prevention. 2008. Select agent regulations. Centers for Disease Control and Prevention, Atlanta, GA. http://www.selertagents.gov.
6. Chen, Y., L. Caruso, B. McClane, D. Fisher, and P. Gupta. 2007. Disruption of a toxin gene by introduction of a foreign gene into the chromosome of Clostridium perfringens using Targetron-induced mutagenesis. Plasmid 58: 182-189.
7. Chen, Y., B. A. McClane, D. J. Fisher, J. I. Rood, and P. Gupta. 2005. Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron. Appl. Environ. Microbiol. 71:7542-7547.
8. Cheung, J. K., M. M. Awad, S. McGowan, and J. I. Rood. 2009. Functional analysis of the VirSR phosphorelay from Clostridium perfringens. PLoS One 4:e5849.
9. Dasgupta, B. R. 1989. The structure of botulinum neurotoxin, p. 53-67. In L. L. Simpson (ed.), Botulinum neurotoxin and tetanus neurotoxin. Academic Press, San Diego, CA.
10. Doyle, M. P. 1991. Evaluating the potential risk from extended-shelf-life refrigerated foods by Clostridium botulinum inoculation studies. Food Technol. 45:154-156.
11. Glass, K. A., and E. A. Johnson. 2004. Antibotulinal activity of process cheese ingredients. J. Food Prot. 67:1765-1769.
12. Glass, K. A., and E. A. Johnson. 2002. Formulating low-acid foods for botulinal safety, p. 323-350. In V. K. Juneja and J. N. Sofos (ed.), Control of foodbome microorganisms. Marcel Dekker, New York, NY.
13. Hatheway, C. L. 1993. Clostridium botulinum and other Clostridia that produce botulinum neurotoxin, p. 3-20. In H. W. Hauschild and K. L. Dodds (ed.), Clostridium botulinum: ecology and control in foods. Marcel Dekker, New York, NY.
14. Hauschild, H. W., and K. L. Dodds (ed.). 1993. Clostridium botulinum: ecology and control in foods. Marcel Dekker, New York, NY.
15. Heap, J. T., S. A. Kuehne, M. Ehsaan, S. T. Cartman, C. M. Cooksley, J. C. Scott, and N. P. Minton. 2009. The ClosTron: mutagenesis in Clostridium refined and streamlined. J. Microbiol. Methods. [Epub ahead of print.] doi: 10.1016/j.mimet.2009.10.018.
16. Heap, J. T., O. J. Pennington, S. T. Cartman, G. P. Carter, and N. P. Minton. 2007. The ClosTron: a universal gene knock-out system for the genus Clostridium. J. Microbiol. Methods 70:452-464.
17. Heap, J. T., O. J. Pennington, S. T. Cartman, and N. P. Minton. 2009. A modular system for Clostridium shuttle plasmids. J. Microbiol. Methods 78:79-85.
18. Johnson, E. A. 2007. Clostridium botulinum, p. 401-421. In M. P. Doyle and L. R. Beuchat (ed.), Food microbiology: fundamentals and frontiers, 3rd ed. ASM Press, "Washington, DC.
19. Johnson, E. A. 2005. Clostridium botulinum and Clostridium tetani, p. 1035-1088. In S. P. Borriello, P. R. Murray, and G. Funke (ed.), Topley and Wilson's microbiology and microbial infections, 8th ed. Hodder Amold, London, United Kingdom.
20. Johnson, E. A., and M. Bradshaw. 2001. Clostridium botulinum and its neurotoxins: a metabolic and cellular perspective. Toxicon 39:1703-1722.
21. Johnson, E. A., W. H. Tepp, M. Bradshaw, R. J. Gilbert, P. E. Cook, and E. D. McIntosh. 2005. Characterization of Clostridium botulinum strains associated with an infant botulism case in the United Kingdom. J. Clin. Microbiol. 43:2602-2607.
22. Karberg, M., H. Guo, J. Zhong, R. Coon. J. Perutka, and A. M. Lambowitz. 2001. Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria. Nat. Biotechnol. 19:1162-1167.
23. Lambowitz, A. M., and S. Zimmerly. 2004. Mobile group II introns. Annu. Rev. Genet. 38:1-35.
24. Malizio, C. J., M. C. Goodnough, and E. A. Johnson. 2000. Purification of Clostridium botulinum type A neurotoxin. Methods Mol. Biol. 145:27-39.
25. Peck, M. W., K. E. Goodburn, R. P. Betts, and S. C. Stringer. 2008. Assessment of the potential for growth and neurotoxin formation by non-proteolytic Clostridium botulinum in short shelf-life commercial foods designed to be stored chilled. Trends Food Sei. Technol. 19:207-216.
26. Purdy, D., T. A. O'Keelle, M. Elmore, M. Herbert, A. McLeod, M. BokoriBrown, A. Ostrowski, and N. P. Minton. 2002. Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier. Mol. Microbiol. 46:439-452.
27. Sakaguchi, G. 1982. Clostridium botulinum toxins. Pharmacol. Ther. 19:165194.
28. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Schantz, E. J., and E. A. Johnson. 1992. Properties and use of botulinum toxin and other microbial neurotoxins in medicine. Microbiol. Rev. 56:80-99.
30. Shao, L., S. Hu, Y. Yang, Y. Gu, J. Chen, W. Jiang, and S. Yang. 2007. Targeted gene disruption by use of a group II intron (Targetron) vector in Clostridium acetobutylicum. Cell Res. 17:963-965.
31. Sharma, S. K., and R. C. Whiting. 2005. Methods for detection of Clostridium botulinum toxin in foods. J. Food Prot. 68:1256-1263.
32. Sleator, R. D., and C. Hill. 2008. Molecular analysis of the microbial food safety implications of food reformulations for improved health. Foodborne Pathog. Dis. 5:499-504.
33. Smith, L. D. S., and H. Sugiyama. 1988. Botulism. The organism, its toxins, the disease, 2nd ed. Charles C Thomas, Springfield, IL.
34. U.S. Food and Drug Administration. 2001. Bacteriological analytical manual. Chapter 17: Clostridium botulinum. http://www.fda.gov/Food/ ScienResearch/Laboratory Methods/BacteriologicalAnalyticalManualBAM/ucm070879 .htm. U.S. Food and Drug Administration, Washington, DC.
35. U.S. Food and Drug Administration. 2005. Principles of thermal processing. U.S. Department of Agriculture Food Safety and Inspection Service, Washington, DC. http://vrow.fsis.usda.gov/PDF/IiaRE-SS-3PrinciplesThermal.pdf.
36. 36. Zhong, J., M. Karberg, and A. M. Lambowitz. 2003. Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker. Nucleic Acids Res. 31: 1656-1664. (Pubitemid 36336567)