The cysteine protease MoAtg4 interacts with MoAtg8 and is required for differentiation and pathogenesis in Magnaporthe oryzae

Autophagy

Volumn 6, Issue 1, 2010, Pages 74-85

  Liu, Tong Bao ^a,b,c   Liu, Xiao Hong ^a   Lu, Jian Ping ^b   Zhang, Lei ^b   Min, Hang ^b   Lin, Fu Cheng ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

^c PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

Autophagy; BiFC; Magnaporthe oryzae; MoAtg4; MoAtg8; Pathogenicity; Protein interaction

Indexed keywords

CYSTEINE DERIVATIVE; CYSTEINE PROTEINASE; GREEN FLUORESCENT PROTEIN; PROTEIN MOATG4; PROTEIN MOATG8; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

ARTICLE; AUTOPHAGY; BARLEY; CELL DEATH; CYTOPLASM; DELETION MUTANT; ENZYME ACTIVITY; FUNGAL DEVELOPMENT; FUNGAL GENE; FUNGAL SPORE GERMINATION; FUNGAL VIRULENCE; FUNGUS GROWTH; FUNGUS HYPHAE; GENE DELETION; GENE EXPRESSION REGULATION; MAGNAPORTHE; MAGNAPORTHE ORYZAE; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RICE; TURGOR PRESSURE;

FUNGI; HORDEUM; MAGNAPORTHE ORYZAE;

EID: 75149188306     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.6.1.10438     Document Type: Article

References (51)

1. Klionsky DJ, Cregg JM, Dunn WA Jr, Emr SD, Sakai Y, Sandoval IV, et al. A unified nomenclature for yeast 3 autophagy-related genes. Dev Cell 2003; 5:539-45.
2. Yorimitsu T, Klionsky DJ. Autophagy: molecular machinery for self-eating. Cell Death Differ 2005; 12:1542-52.
3. Levine B. Eating oneself and uninvited guests: autophagy-related pathways in cellular defense. Cell 2005; 120:159-62.
4. Reggiori F, Klionsky DJ. Autophagy in the eukaryotic cell. Eukaryot Cell 2002; 1:11-21.
5. Klionsky DJ. The molecular machinery of autophagy: unanswered questions. J Cell Sci 2005; 118:7-18.
6. Ichimura Y, Kirisako T, Takao T, Satomi Y, Shimonishi Y, Ishihara N, et al. A ubiquitin-like system mediates protein lipidation. Nature 2000; 408:488-92.
7. Ohsumi Y. Molecular dissection of autophagy: two ubiquitinlike systems. Nat Rev Mol Cell Biol 2001; 2:211-6.
8. Mizushima N, Yoshimori T, Ohsumi Y. Role of the Apg12 conjugation system in mammalian autophagy. Int J Biochem Cell Biol 2003; 35:553-61.
9. nd Ed. Commonwealth Mycological Institute, Kew UK.
10. Ebbole DJ. Magnaporthe as a model for understanding host-pathogen interactions. Annu Rev Phytopathol 2007; 45:437-56.
11. Dean RA, Talbot NJ, Ebbole DJ, Farman ML, Mitchell TK, Orbach MJ, et al. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 2005; 434:980-6.
12. Bourett TM, Howard RJ. In vitro development of penetration structures in the rice blast fungus Magnaporthe grisea. Can J Bot 1990; 68:329-42.
13. Howard RJ, Ferrari MA, Roach DH, Money NP. Penetration of hard substrates by a fungus employing enormous turgor pressures. Proc Natl Acad Sci USA 1991; 88:11281-4.
14. Veneault-Fourrey C, Barooah M, Egan M, Wakley G, Talbot NJ. Autophagic fungal cell death is necessary for infection by the rice blast fungus. Science 2006; 312:580-3.
15. Deng YZ, Ramos-Pamplona M, Naqvi NI. Autophagyassisted glycogen catabolism regulates asexual differentiation in Magnaporthe oryzae. Autophagy 2009; 5:1-11.
16. Liu XH, Lu JP, Zhang L, Dong B, Min H, Lin FC. Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis. Eukaryot Cell 2007; 6:997-1005.
17. Dong B, Liu XH, Lu JP, Zhang FS, Gao HM, Wang HK, Lin FC. MgAtg9 trafficking in Magnaporthe oryzae. Autophagy 2009; 5:1-8.
18. Lu JP, Liu XH, Feng XX, Min H, Lin FC. An autophagy gene, MgATG5, is required for cell differentiation and pathogenesis in Magnaporthe oryzae. Curr Genet 2009; 55:461-73.
19. Talbot NJ, Ebbole DJ, Hamer JE. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 1993; 5:1575-90.
20. + exchanger sod2 in Saccharomyces cerevisiae. Mol Cell Biochem 2008; 319:79-86.
21. Lu JP, Liu TB, Yu XY, Lin FC. Representative appressorium stage cDNA library of Magnaporthe grisea. J Zhejiang Univ Sci B 2005; 6:132-6.
22. Liu XH, Liu TB, Lin FC. Monitoring autophagy in Magnaporthe oryzae. Methods Enzymol 2008; 451:271-94.
23. Schägger H. Tricine-SDS-PAGE. Nat protoc 2006; 1:16-23.
24. Pall MC, Brunelli P. A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites. Fungal Genet Newsl 1993; 40:59-62.
25. Liu TB, Lu JP, Liu XH, Min H, Lin FC. A simple and effective method for total RNA isolation of appressoria in Magnaporthe oryzae. J Zhejiang Univ Sci B 2008; 9:811-7.
26. Lu JP, Feng XX, Liu XH, Lu Q, Wang HK, Lin FC. Mnh6, a nonhistone protein, is required for fungal development and pathogenicity of Magnaporthe grisea. Fungal Genet Biol 2007; 44:819-29.
27. Soundararajan S, Jedd G, Li X, Ramos-Pamplona M, Chua NH, Naqvi NI. Woronin body function in Magnaporthe grisea is essential for efficient pathogenesis and for survival during nitrogen starvation stress. Plant Cell 2004; 16:1564-74.
28. rd ed. Cold Spring Harbor Laboratory Press, New York NY.
29. Dufresne M, Osbourn AE. Definition of tissue-specific and general requirements for plant infection in a phytopathogenic fungus. Mol Plant-Microbe Interact 2001; 14:300-7.
30. Barrett AJ, Rawlings ND. Evolutionary Lines of Cysteine Peptidases. Biological Chemistry 2001; 382:727-33.
31. Matsuura A, Tsukada M, Wada Y, Ohsumi Y. Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae. Gene 1997; 192:245-50.
32. Lang T, Schaeffeler E, Bernreuther D, Bredschneider M, Wolf DH, Thumm M. Aut2p and Aut7p, two novel microtubuleassociated proteins are essential for delivery of autophagic vesicles to the vacuole. EMBO J 1998; 17:3597-607.
33. Kirisako T, Ichimura Y, Okada H, Kabeya Y, Mizushima N, Yoshimori T, et al. The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway. J Cell Biol 2000; 151:263-76.
34. Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZip and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 2002; 9:789-98.
35. Hoff B, Kuck U. Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum. Curr Genet 2005; 47:132-8.
36. Zhao X, Xu JR. A highly conserved MAPK-docking site in Mst7 is essential for Pmk1 activation in Magnaporthe grisea. Mol Microbiol 2007; 63:881-94.
37. Bonman JM, Vergel DDT, Khin MM. Physiologic specialization of Pyricularia oryzae in the Philippines. Plant Dis 1986; 70:767-9.
38. Yoshimoto K, Hanaoka H, Sato S, Kato T, Tabata S, Noda T, Ohsumia Y. Processing of ATG8s, ubiquitinlike proteins, and their deconjugation by ATG4s are essential for plant autophagy. Plant Cell 2004; 16:2967-83.
39. Rose TL, Bonneau L, Der C, Marty-Mazars D, Marty F. Starvation-induced expression of autophagy-related genes in Arabidopsis. Biol Cell 2006; 98:53-67.
40. Yoshimura K, Shibata M, Koike M, Gotoh K, Fukaya M, Watanabe M, Uchiyama Y. Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues. Autophagy 2006; 2:200-8.
41. Kerppola TK. Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells. Nature Protocols 2006a; 1:1278-86.
42. Kerppola TK. Visualization of molecular interaction by fluorescence complementation. Nat Rev Mol Cell Biol 2006b; 7:449-56.
43. Tsukada M, Ohsumi Y. Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 1993; 333:169-74.
44. Straub M, Bredschneider M, Thumm M. AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae. J Bacteriol 1997; 179:3875-83.
45. Palmer GE, Kelly MN, Sturtevant JE. Autophagy in the pathogen Candida albicans. Microbiology 2007; 153:51-8.
46. Pinan-Lucarré B, Paoletti M, Dementhon K, Coulary-Salin B, Clavé C. Autophagy is induced during cell death by incompatibility and is essential for differentiation in the filamentous fungus Podospora anserine. Mol Microbiol 2003; 47:321-33.
47. Pinan-Lucarré B, Balguerie A, Clave C. Accelerated cell death in Podospora autophagy mutants. Eukaryot Cell 2005; 4:1765-74.
48. Kikuma T, Ohneda M, Arioka M, Kitamoto K. Functional analysis of the ATG8 homologue Aoatg8 and role of Autophagy in differentiation and germination in Aspergillus oryzae. Eukaryot Cell 2006; 5:1328-36.
49. Hu G, Hacham M, Waterman SR, Panepinto J, Shin S, Liu X, et al. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans. J Clin Invest 2008; 118:1186-97.
50. Shoji J, Maruyama J, Arioka M, Kitamoto K. Development of Aspergillus oryzae thiA promoter as a tool for molecular biological studies. FEMS Microbiol Lett 2005; 244:41-6.
51. Liu TB, Chen GQ, Min H, Lin FC. MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae. J Zhejiang Univ Sci B 2009; 10:434-44.