Evaluation of the impact of lipid fouling during the chromatographic purification of virus-like particles from Saccharomyces cerevisiae

Journal of Chemical Technology and Biotechnology

Volumn 85, Issue 2, 2010, Pages 209-215

  Jin, Jing ^a   Chhatre, Sunil ^a   Titchener Hooker, Nigel J ^a   Bracewell, Daniel G ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Fouling; Hydrophobic interaction chromatography; Lipid; Purification; Virus like particle; Yeast

Indexed keywords

BINDING CAPACITIES; CELL DISRUPTION; CELLULAR COMPONENTS; CHROMATOGRAPHIC PURIFICATION; CHROMATOGRAPHY COLUMNS; CLEAN IN PLACES; COLUMN PERFORMANCE; FOULING PROBLEMS; HOST CELLS; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; LOSS OF PERFORMANCE; OPERATIONAL CYCLE; REPEATED LOADING; SACCHAROMYCES CEREVISIAE; TRIACYLGLYCEROLS; VIRUS-LIKE PARTICLE; VIRUS-LIKE PARTICLES; YEAST EXPRESSION;

CHROMATOGRAPHIC ANALYSIS; FOULING; GLYCEROL; HYDROPHOBICITY; LIPIDS; PURIFICATION; VIRUSES; YEAST;

HYDROPHOBIC CHROMATOGRAPHY;

ERGOSTEROL; PHOSPHOLIPID; TRIACYLGLYCEROL; TRITON X 100;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; BIOFOULING; COLUMN CHROMATOGRAPHY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FERMENTATION MEDIUM; FOOD COMPOSITION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; LIPID COMPOSITION; NONHUMAN; NUTRIENT AVAILABILITY; PERFORMANCE MEASUREMENT SYSTEM; SACCHAROMYCES CEREVISIAE; VIRUS LIKE AGENT;

SACCHAROMYCES CEREVISIAE;

EID: 75149183300     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.2290     Document Type: Article

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