Hepatitis C virus budding at lipid droplet-associated ER membrane visualized by 3D electron microscopy

Histochemistry and Cell Biology

Volumn 130, Issue 3, 2008, Pages 561-566

  Roingeard, Philippe ^a   Hourioux, Christophe ^a   Blanchard, Emmanuelle ^a   Prensier, Gérard ^b  

^a UNIVERSITÉ DE TOURS   (France)

^b UNIVERSITÉ BLAISE PASCAL   (France)

Author keywords

3D; Electron microscopy (EM); HCV; Lipid droplet; Virus morphogenesis

Indexed keywords

CORE PROTEIN; FAT DROPLET;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING; VIRUS LIKE AGENT; VIRUS MORPHOGENESIS;

ANIMALS; CELL LINE; CRICETINAE; ENDOPLASMIC RETICULUM; HEPACIVIRUS; INTRACELLULAR MEMBRANES; LIPID METABOLISM; LIPIDS; MICROSCOPY, IMMUNOELECTRON;

HEPATITIS C VIRUS;

EID: 50249107793     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-008-0447-2     Document Type: Article

References (15)

1. M Ait-Goughoulte C Hourioux R Patient S Trassard D Brand P Roingeard 2006 Core protein cleavage by signal peptide peptidase is required for hepatitis C virus-like particle assembly J Gen Virol 87 855 860
2. E Blanchard C Hourioux D Brand M Ait-Goughoulte A Moreau S Trassard PY Sizaret F Dubois P Roingeard 2003 Hepatitis C virus-like particle budding: role of the core protein and importance of its Asp111 J Virol 77 10131 10138
3. S Boulant P Targett-Adams J McLauchlan 2007 Disrupting the association of hepatitis C virus core protein with lipid droplets correlates with a loss in production of infectious virus J Gen Virol 88 2204 2213
4. C Hourioux M Ait-Goughoulte R Patient D Fouquenet F Arcanger-Doudet D Brand A Martin P Roingeard 2007 Core protein domains involved in hepatitis C virus-like particle assembly and budding at the endoplasmic reticulum membrane Cell Microbiol 4 1014 1027
5. C Hourioux R Patient A Morin E Blanchard A Moreau S Trassard B Giraudeau P Roingeard 2007 The genotype 3-specific hepatitis C virus core protein residue phenylalanine 164 increases steatosis in an in vitro cellular model Gut 56 1302 1308
6. H Huang F Sun DM Owen W Li Y Chen M Gale Jr J Ye 2007 Hepatitis C virus production by human hepatocytes dependent on assembly and secretion of very low-density lipoproteins Proc Natl Acad Sci USA 104 5848 5453
7. JR Kremer DN Mastronarde JR McIntosh 1996 Computer visualization of three-dimensional image data using IMOD J Struct Biol 116 71 76
8. BD Lindenbach MJ Evans AJ Syder B Wolk TL Tellinghuisen CC Liu T Maruyama RO Hynes DR Burton JA McKeating CM Rice 2005 Complete replication of hepatitis C virus in cell culture Science 309 623 626
9. Y Miyanari K Atsuzawa N Usuda K Watashi T Hishiki M Zayas R Bartenshlager T Wakita M Hijikata K Shimotohno 2007 The lipid droplet is an important organelle for hepatitis C virus production Nat Cell Biol 9 1089 1097
10. P Roingeard C Hourioux E Blanchard D Brand M Ait-Goughoulte 2004 Hepatitis C virus ultrastructure and morphogenesis Biol Cell 96 103 108
11. Y Rouillé F Helle D Delgrange P Roingeard C Voisset E Blanchard S Belouzard JA McKeating AH Patel G Maertens T Wakita C Wychowski J Dubuisson 2006 Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus J Virol 80 2832 2841
12. A Shavinskaya S Boulant F Penin J McLauchlan R Bartenschlager 2007 The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly J Biol Chem 282 37158 37169
13. P Targett-Adams S Boulant J McLauchlan 2008 Visualization of double-stranded RNA in cells supporting hepatitis C virus RNA replication J Virol 82 2182 2195
14. T Wakita T Pietschmann T Kato T Date M Miyamoto Z Zhao K Murthy A Habermann HG Krausslich M Mizokami R Bartenschlager TJ Liang 2005 Production of infectious hepatitis C virus in tissue culture from a cloned viral genome Nat Med 11 791 796
15. J Zhong P Gastaminza G Cheng S Kapadia T Kato DR Burton SF Wieland SL Uprichard T Wakita FV Chisari 2005 Robust hepatitis C virus infection in vitro Proc Natl Acad Sci USA 102 9294 9299