Identification of amino acids in the pore region of Kv1.2 potassium channel that regulate its glycosylation and cell surface expression

Journal of Neurochemistry

Volumn 112, Issue 4, 2010, Pages 913-923

  Utsunomiya, Iku ^a   Tanabe, Shinya ^a   Terashi, Tomonori ^a   Ikeno, Souichi ^a   Miyatake, Tadashi ^b   Hoshi, Keiko ^a   Taguchi, Kyoji ^a  

^a SHOWA PHARMACEUTICAL UNIVERSITY   (Japan)

^b Japan Science and Technology Agency   (Japan)

Author keywords

Glycosylation; Point mutation; Pore region; Potassium channel; Surface expression; Trafficking

Indexed keywords

ALANINE; AMINO ACID; ARGININE; LYSINE; POTASSIUM CHANNEL KV1.2; POTASSIUM CHANNEL KV1.4; PROLINE; SERINE; THREONINE; TYROSINE; VALINE;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL SURFACE; CHANNEL GATING; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS;

AMINO ACIDS; ANIMALS; CELL MEMBRANE; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION REGULATION; GLYCOSYLATION; ION CHANNEL GATING; KV1.2 POTASSIUM CHANNEL; MEMBRANE POTENTIALS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PATCH-CLAMP TECHNIQUES; PROTEIN TRANSPORT; TRANS-GOLGI NETWORK; TRANSFECTION;

EID: 75149169914     PISSN: 00223042     EISSN: 14714159     Source Type: Journal    
DOI: 10.1111/j.1471-4159.2009.06523.x     Document Type: Article

References (31)

1. Bradford M. M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem. 72, 248 254.
2. Christie M. J., North R. A., Osborne P. B., Douglass J. Adelman J. P. (1990) Heteropolymeric potassium channels expressed in Xenopus oocytes from cloned subunits. Neuron 4, 405 411.
3. + conduction and selectivity. Science 280, 69 77.
4. Fujita T., Utsunomiya I., Ren J., Matsushita Y., Kawai M., Sasaki S., Hoshi K., Miyatake T. Taguchi K. (2006) Glycosylation and cell surface expression of Kv1.2 potassium channel are regulated by determinants in the pore region. Neurochem. Res. 31, 589 896.
5. + channels. Science 251, 942 944.
6. Jan L. Y. Jan Y. N. (1997) Cloned potassium channels from eukaryotes and prokaryotes. Ann. Rev. Neurosci. 20, 91 123.
7. Li T., Takimoto K. Levitan E. S. (2000) Surface expression of Kv1 channels is governed by a C-terminal motif. J. Biol. Chem. 275, 11597 11602.
8. Ma D., Zerangue N., Lin Y.-F., Collins A., Yu M., Jan Y. N. Jan L. Y. (2001) Role of ER export signals in controlling surface potassium channel numbers. Science 291, 316 319.
9. MacKinnon R. (1991) Determination of the subunit stoichiometry of a voltage-activated potassium channel. Nature 350, 232 235.
10. Manganas L. N. Trimmer J. S. (2000) Subunit composition determines Kv1 potassium channel surface expression. J. Biol. Chem. 275, 29685 29693.
11. Manganas L. N. Trimmer J. S. (2004) Calnexin regulates mammalian Kv1 channel trafficking. Biochem. Biophys. Res. Commun. 322, 577 584.
12. Manganas L. N., Wang Q., Scannevin R. H., Antonucci D. E., Rhodes K. J. Trimmer J. S. (2001) Identification of a trafficking determinant localized to the Kv1 potassium channel pore. Proc. Natl Acad. Sci. USA 98, 14055 14059.
13. McKinnon D. (1989) Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family. J. Biol. Chem. 264, 8230 8236.
14. Papazian D. M., Timpe L. C., Jan Y. N. Jan L. Y. (1991) Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence. Nature 349, 305 310.
15. Rasband M. N. (2004) It's "juxta" potassium channel. J. Neurosci. Res. 76, 749 757.
16. Rasband M. N., Park E. W., Vanderah T. W., Lai J., Porreca F. Trimmer J. S. (2001) Distinct potassium channels on pain-sensing neurons. Proc. Natl Acad. Sci. USA 98, 13373 13378.
17. + channel complexes. J. Neurosci. 17, 8246 8258.
18. Santacruz-Toloza L., Huang Y., John S. A. Papazian D. M. (1994) Glycosylation of Shaker potassium channel protein in insect cell culture and in Xenopus oocytes. Biochemistry 33, 5607 5613.
19. + channel. Biochemistry 35, 12133 12140.
20. + channel surface expression through effects early in biosynthesis. Neuron 16, 843 852.
21. Stuhmer W., Ruppersberg J. P., Schroter K. H., Sakmann B., Stocker M., Giese K. P., Perschke A., Baumann A. Pongs O. (1989) Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain. EMBO J. 8, 3235 3244.
22. Utsunomiya I., Yoshihashi E., Tanabe S., Nakatani Y., Ikejima H., Miyatake T., Hoshi K. Taguchi K. (2008) Expression and localization of Kv1 potassium channels in rat dorsal and ventral spinal roots. Exp. Neurol. 210, 51 58.
23. Valleio A. N., Pogulis R. J. Pease L. R. (1994) In vitro synthesis of novel genes: mutagenesis and recombination by PCR. PCR Methods Appl. 4, 123 130.
24. Watanabe I., Zhu J., Recio-Pinto E. Thornhill W. B. (2004) Glycosylation affects the protein stability and cell surface expression of Kv1.4 but not Kv1.1 potassium channels. J. Biol. Chem. 279, 8879 8885.
25. Watanabe I., Zhu J., Sutachan J. J., Gottschalk A., Recio-Pinto E. Thornhill W. B. (2007) The glycosylation state of Kv1.2 potassium channels affects trafficking, gating, and simulated action potentials. Brain Res. 1144, 1 18.
26. + channel. Science 251, 939 942.
27. Zhu J., Watanabe I., Gomez B. Thornhill W. B. (2001) Determinants involved in Kv1 potassium channel folding in the endoplasmic reticulum, glycosylation in the Golgi, and cell surface expression. J. Biol. Chem. 276, 39419 39427. (Pubitemid 37371320)
28. Zhu J., Watanabe I., Gomez B. Thornhill W. B. (2003a) Heteromeric Kv1 potassium channel expression: amino acid determinants involved in processing and trafficking to the cell surface. J. Biol. Chem. 278, 25558 25567.
29. Zhu J., Watanabe I., Gomez B. Thornhill W. B. (2003b) Trafficking of Kv1.4 potassium channels: interdependence of a pore region determinant and a cytoplasmic C-terminal VXXSL determinant in regulating cell-surface trafficking. Biochem. J. 375, 761 768.
30. Zhu J., Watanabe I., Poholek A., Koss M., Gomez B., Yan C., Recio-Pinto E. Thornhill W. B. (2003c) Allowed N-glycosylation sites on the Kv1.2 potassium channel S1-S2 linker: implications for linker secondary structure and the glycosylation effect on channel function. Biochem. J. 375, 769 775.
31. Zhu J., Gomez B., Watanabe I. Thornhill W. B. (2005) Amino acids in the pore region of Kv1 potassium channels dictate cell-surface protein levels: a possible trafficking code in the Kv1 subfamily. Biochem. J. 388, 355 362.