Real-time LightCycler polymerase chain reaction and melting temperature analysis for identification of clinically important Candida spp.

Journal of Microbiology, Immunology and Infection

Volumn 42, Issue 4, 2009, Pages 290-295

  Khan, Ziauddin ^a   Mustafa, Abu Salim ^a   Alam, Fasahat Fakhar ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Candida; Laboratory techniques and procedures; Polymerase chain reaction; Transition temperature

Indexed keywords

FLUORESCENT DYE; GENOMIC DNA;

ARTICLE; CANDIDA; CANDIDA ALBICANS; CANDIDA DUBLINIENSIS; CANDIDA GLABRATA; CANDIDA KRUSEI; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; CANDIDEMIA; CANDIDIASIS; CHLAMYDOSPORE; FUNGAL STRAIN; FUNGUS IDENTIFICATION; GENE AMPLIFICATION; INVASIVE CANDIDIASIS; MELTING POINT; NONHUMAN; PROGNOSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENTIATION;

CANDIDA; CANDIDIASIS; DNA, FUNGAL; FUNGEMIA; HUMANS; MYCOLOGICAL TYPING TECHNIQUES; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY; TRANSITION TEMPERATURE;

EID: 74849119454     PISSN: 16841182     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Eggimann P, Garbino J, Pittet D. Epidemiology of Candida species infections in critically ill non-immunosuppressed patients. Lancet Infect Dis. 2003;3:685-702.
2. Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev. 2007;20:133-63.
3. Ahmad S, Khan Z, Mustafa AS, Khan ZU. Epidemiology of Candida colonization in an intensive care unit of a teaching hospital in Kuwait Med Mycol. 2003;41:487-93.
4. Falagas ME, Apostolou KE, Pappas VD. Attributable mortality of candidemia: a systematic review of matched cohort and case-control studies. Eur J Clin Microbiol Infect Dis. 2006;25:419-25.
5. Pfaller MA, Diekema DJ, Gibbs DL, Newell VA, Nagy E, Dobiasova S, et al; Global Antifungal Surveillance Group. Candida krusei, a multidrug-resistant opportunistic fungal pathogen: geographic and temporal trends from the ARTEMIS DISK Antifungal Surveillance Program, 2001 to 2005. J Clin Microbiol. 2008;46:515-21.
6. Fridkin SK. The changing face of fungal infections in health care settings. Clin Infect Dis. 2005;41:1455-60.
7. Ellepola AN, Morrison CJ. Laboratory diagnosis of invasive candidiasis. J Microbiol. 2005;43:65-84.
8. Bretagne S, Costa JM. Towards a molecular diagnosis of invasive aspergillosis and disseminated candidosis. FEMS Immunol Med Microbiol. 2005;45:361-8.
9. Yeo SF, Wong B. Current status of non-culture methods for diagnosis of invasive fungal infections. Clin Microbiol Rev. 2002;15:465-84.
10. Khan ZU, Mustafa AS. Detection of Candida species by polymerase chain reaction (PCR) in blood samples of experimentally infected mice and patients with suspected candidemia. Microbiol Res. 2001;156:95-102.
11. Ahmad S, Khan Z, Mustafa AS, Khan ZU. Seminested PCR for diagnosis of candidemia comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol. 2002;40:2483-9.
12. Alam FF, Mustafa AS, Khan ZU. Comparative evaluation of (1, 3)-beta-D-glucan, mannan and anti-mannan antibodies, and Candida species-specific snPCR in patients with candidemia. BMC Infect Dis. 2007;7:103-11.
13. Chen YC, Eisner JD, Kattar MM, Rassoulian-Barrett SL, Lafe K, Bui U, et al. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J Clin Microbiol. 2001;9:4042-51.
14. Fujita SI, SendaY, Nakaguchi S, Hashimoto T. Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains. J Clin Microbiol. 2001;39:3617-22.
15. Putignani L, Paglia MG, Bordi E, Nebuloso E, Pucillo LP, Visca P. Identification of clinically relevant yeast species by DNA sequence analysis of the D2 variable region of the 25-28S rRNA gene. Mycoses. 2008;51:209-27.
16. Loeffler J, Henke N, Hebart H, Schmidt D, Hagmeyer L, Schumacher U, et al. Quantification of fungal DNA by using fluorescence resonance energy transfer and the LightCycler System. J Clin Microbiol. 2000;38:586-90.
17. Guiver M, Levi K, Oppenheim BA. Rapid identification of Candida species by TaqMan PCR. J Clin Pathol. 2001;54:362-6.
18. Spiess B, Buchheidt D, Baust C, Skladny H, Seifarth W, Zeilfelder U, et al. Development of a LiglitCycler PCR assay for detection and quantification of Aspergillus fumigates DNA in clinical samples from neutropenic patients. J Clin Microbiol. 2003;41:1811-8.
19. White PL, Shetty A, Barnes RA. Detection of seven Candida species using the Light-Cycler System. J Med Microbiol. 2003;52:229-38.
20. Klingspor L, Jalal S. Molecular detection and identification of Candida and Aspergillus spp. from clinical samples using real-time PCR. Clin Microbiol Infect. 2006;12:745-3.
21. Maaroufi Y, Ahariz N, Husson M, Crokaert F. Comparison of different methods of isolation of DNA of commonly encountered Candida species and its quantitation by using a real-time PCR-based assay. J Clin Microbiol. 2004;42:3159-63.
22. Fujita S, Lasker BA, Lott TJ, Reiss F, Morrison CJ. Microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species in blood. J Clin Microbiol. 1995;33:962-7.
23. Blot S, Vandewoude K. Management of invasive candidiasis in critically ill patients. Drugs. 2004;64:2159-75.
24. Gutierrez J, Morales P, Gonzalez MA, Quindos G. Candida dubliniensis, a new fungal pathogen. J Basic Microbiol, 2002;42:207-27.
25. Hsu MC, Chen KW, Lo HJ, Chen YC, Liao MH, Lin YH, et al. Species identification of medically important fungi by use of real-time LightCycler PCR. J Med Microbiol. 2003;52:1071-6.