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Volumn 132, Issue 1, 2010, Pages 67-69

Optical imaging of mammary and prostate tumors in living animals using a synthetic near infrared zinc(II)-dipicolylamine probe for anionic cell surfaces

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL MODEL; ANIONIC MEMBRANES; CELL SURFACES; EX-VIVO; FLUORESCENT IMAGING; HISTOLOGICAL ANALYSIS; IN-VITRO; IN-VIVO; LIVING ANIMALS; MAMMARY TUMORS; NEAR INFRARED; OPTICAL IMAGING; PROSTATE TUMOR;

EID: 74849090694     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja908467y     Document Type: Article
Times cited : (162)

References (43)
  • 18
    • 58049202245 scopus 로고    scopus 로고
    • For recent reviews of molecular recognition using Zn-DPA complexes, see: a
    • For recent reviews of molecular recognition using Zn-DPA complexes, see: (a) Sakamoto, T.; Ojida, A.; Hamachi, I. Chem. Commun. 2009, 141-152.
    • (2009) Chem. Commun , pp. 141-152
    • Sakamoto, T.1    Ojida, A.2    Hamachi, I.3
  • 32
    • 74849095123 scopus 로고    scopus 로고
    • Control 3 is the near-IR fluorophore, Indocyanine Green (ICG), which is approved for use in humans. It associates strongly with plasma proteins and is rapidly cleared by the liver. ICG and non-targeted ICG-conjugates typically exhibit modest tumor targeting ability (T/NT < 2) due to enhanced permeation-retention (EPR) effects. See, for example: (a) Becker, A.; Riefke, B.; Ebert, B.; Sukowski, U.; Rinneberg, H.; Semmler, W.; Licha, K. Photochem. Photobiol. 2000, 72, 234-241.
    • Control 3 is the near-IR fluorophore, Indocyanine Green (ICG), which is approved for use in humans. It associates strongly with plasma proteins and is rapidly cleared by the liver. ICG and non-targeted ICG-conjugates typically exhibit modest tumor targeting ability (T/NT < 2) due to enhanced permeation-retention (EPR) effects. See, for example: (a) Becker, A.; Riefke, B.; Ebert, B.; Sukowski, U.; Rinneberg, H.; Semmler, W.; Licha, K. Photochem. Photobiol. 2000, 72, 234-241.
  • 39
    • 38849154533 scopus 로고    scopus 로고
    • Direct comparison of ex vivo mean pixel intensities for probe 1 and control fluorophore 2 is possible because they have essentially identical brightness and the same fraction of light absorption/scattering by the excised tissue. The average ex vivo tumor intensity from rat dosed with probe 1 is 16-fold higher than rat dosed with control fluorophore 3 (ICG, see ref 20, however, this is only a qualitative measure of relative tumor selectivity because the brightness of 3 in tumor tissue is somewhat uncertain. As expected, the ex vivo imaging with probe 1 shows higher tumor selectivity than the in vivo imaging (T/NT) because the fluorescence signal from the implanted tumor is attenuated by the surrounding skin and tissue, 25) At this point, we cannot rule out that a fraction of probe 1 is targeting the anionic epithelial cell surfaces that line the tumor vasculature. For further discussion of this possibility, see ref 10. Another topic for
    • Direct comparison of ex vivo mean pixel intensities for probe 1 and control fluorophore 2 is possible because they have essentially identical brightness and the same fraction of light absorption/scattering by the excised tissue. The average ex vivo tumor intensity from rat dosed with probe 1 is 16-fold higher than rat dosed with control fluorophore 3 (ICG, see ref 20); however, this is only a qualitative measure of relative tumor selectivity because the brightness of 3 in tumor tissue is somewhat uncertain. As expected, the ex vivo imaging with probe 1 shows higher tumor selectivity than the in vivo imaging (T/NT) because the fluorescence signal from the implanted tumor is attenuated by the surrounding skin and tissue. (25) At this point, we cannot rule out that a fraction of probe 1 is targeting the anionic epithelial cell surfaces that line the tumor vasculature. For further discussion of this possibility, see ref 10. Another topic for further study is whether probe 1 maintains its zinc(II) ions at the in vivo binding site. For evidence that this is the case in vitro, see: DiVittorio, K. M.; Leevy, W. M.; O'Neil, E. J.; Johnson, J. R.; Vakulenko, S.; Morris, J. D.; Rosek, K. D.; Serazin, N.; Hilkert, S.; Hurley, S.; Marquez, M.; Smith, B. D. ChemBioChem 2008, 9, 286-293.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.