Intrabodies, potent tools to unravel the function and dynamics of intracellular proteins;Utilisation des intrabodies: De l'étude des protéines intracellulaires à l'immunisation thérapeutique

Medecine/Sciences

Volumn 25, Issue 12, 2009, Pages 1173-1176

  Moutel, Sandrine ^a   Perez, Franck ^a  

^a INSTITUT CURIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 74749088072     PISSN: 07670974     EISSN: None     Source Type: Journal    
DOI: 10.1051/medsci/200925121173     Document Type: Review

References (17)

1. Kreis TE. Microinjected antibodies against the cytoplasmic domain of vesicularstomatitis virus glycoprotein block its transport to the cell surface. EMBOJ 1986 ; 5 : 931-41.
2. Orlandi R, Güssow DH, Jones PT, Winter G. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proc Natl Acad Sci USA 1989 ; 86: 3833-7.
3. Biocca S, Neuberger MS, Cattaneo A. Expression and targeting of intracellular antibodies in mammalian cells. EMBOJ 1990 ; 9 : 101-8.
4. Biocca S, Ruberti F, Tafani M, et al. Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria. Biotechnology (NY) 1995 ; 13 : 1110-5.
5. Philibert P, Stoessel A, Wang W, et al. A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm. BMC Biotechnol 2007; 7: 81.
6. Visintin M, Settanni G, Maritan A, et al. The intracellular antibody capture technology (IACT) : towards a consensus sequence for intracellular antibodies. J Mol Biol 2002 ; 317: 73-83.
7. Holliger P, Hudson PJ. Engineered antibody fragments and the rise of single domains. Mat Biotechnol 2005 ; 23 : 1126-36.
8. Tanaka T, Rabbitts TH. Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds. J MoI Biol 2008 ; 376 : 749-57.
9. Saerens D, Pellis M, Loris R, et al. Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies. J Mol Biol 2005 ; 352 : 597-607.
10. Nizak C, Martin-Lluesma S, Moutel S, et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo. Traffic 2003 ; 4 : 739-53.
11. Nizak C, Monier S, del Neiy E, et al. Recombinant antibodies to the small GTPase Rab6 as conformation sensors. Science 2003 ; 300 : 984-7.
12. Dimitrov A, Quesnoit M, Moutel S, et al. Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues. Science 2008 ; 322 : 1353-6.
13. Rothbauer U, Zolghadr K, Tillib S, et al. Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat Methods 2006 ; 3 : 887-9.
14. Marasco WA, Haseltine WA, Chen S. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gpl20 single-chain antibody. Proc Natl Acad Sci USA 1993 ; 90: 7889-93.
15. Cochet O, Kenigsberg M, Delumeau I, et al. Intracellular expression of an antibody fragment-neutralizing p21 Ras promotes tumor regression. Cancer Res 1998 ; 58 : 1170-6.
16. Cardinale A, Biocca S. The potential of intracellular antibodies for therapeutic targeting of protein-misfolding diseases. Trends Mol Med 2008 ; 14 : 373-80.
17. Lobato MN, Rabbitts TH. Intracellular antibodies and challenges facing their use as therapeutic agents. Trends Mol Med 2003 ; 9: 390-6.