Morphological, toxicological and molecular characterization of a benthic Nodularia isolated from Atlantic estuarine environments

Research in Microbiology

Volumn 161, Issue 1, 2010, Pages 9-17

  Lopes, Viviana R ^a,b   Antunes, Agostinho ^a   Welker, Martin ^c   Martins, Rosário F ^a,d   Vasconcelos, Vítor M ^a,b  

^a UNIVERSITY OF PORTO   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

^c TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^d POLYTECHNIC INSTITUTE OF PORTO   (Portugal)

Author keywords

16S ribosomal RNA; Atlantic ecosystems; Ecotoxicity; Estuary; Nodularia sp.

Indexed keywords

BACTERIAL TOXIN; CYANOTOXIN; GLYCOLIPID; METHANOL; NODULARIN; NODULARIN SYNTHETASE; RNA 16S; SYNTHETASE; UNCLASSIFIED DRUG; WATER;

ARTEMIA; ARTICLE; BACTERIAL STRAIN; BACTERIUM EXAMINATION; BACTERIUM ISOLATE; BENTHOS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ECOTOXICOLOGY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESTUARY; GENE SEQUENCE; GENETIC ANALYSIS; GENOTOXICITY; LC 50; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROBIAL MORPHOLOGY; NODULARIA; NODULARIA HARVEYANA; NODULARIA SPHAEROCARPA; NODULARIA SPUMIGENA; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYLOGENY; PORTUGAL; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM; TOXIN SYNTHESIS;

ANIMALS; ARTEMIA; ATLANTIC OCEAN; BACTERIAL TOXINS; CLUSTER ANALYSIS; DNA, BACTERIAL; DNA, RIBOSOMAL; ESCHERICHIA COLI; GEOLOGIC SEDIMENTS; GLYCOLIPIDS; MOLECULAR SEQUENCE DATA; NODULARIA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SALMONELLA TYPHIMURIUM; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ARTEMIA; ARTEMIA SP.; ESCHERICHIA COLI; NODULARIA; NODULARIA HARVEYANA; NODULARIA SPHAEROCARPA; NODULARIA SPUMIGENA; SALMONELLA TYPHIMURIUM;

EID: 74649085705     PISSN: 09232508     EISSN: 17697123     Source Type: Journal    
DOI: 10.1016/j.resmic.2009.11.001     Document Type: Article

References (39)

1. Allen M.M., and Stanier R.Y. Growth and division of some unicellular blue-green algae. J. Gen. Microbiol. 51 (1968) 199-202
2. Barrios-Llerena M.E., Burja A.M., and Wright P.C. Genetic analysis of polyketide synthase and peptide synthetase genes in cyanobacteria as a mining tool for secondary metabolites. J. Ind. Microbiol. Biotechnol. 34 (2007) 443-456
3. Boyer S.L., Flechtner V.R., and Johansen J.R. Is the 16S-23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria. Mol. Biol. Evol. 18 (2001) 1057-1069
4. Caldwell G.S., Bentley M.G., and Olive P.J.W. The use of the brine shrimp (Artemia salina) bioassay to assess the toxicity of diatom extracts and short chain aldehydes. Toxicon 42 (2003) 301-306
5. Carmichael W.W. The cyanotoxins. Adv. Bot. Res. 27 (1997) 211-256
6. Castenholz R.W. Phylum BX Cyanobacteria oxygenic photosynthetic bacteria. In: Garrity G., Boone D.R., and Castenholz R.W. (Eds). Bergey's Manual of Systematic Bacteriology. second ed. The Archaea and the Deeply Branching and Phototropic Bacteria vol. 1 (2001), Springer-Verlag, New York 473-599
7. Francis G. Poisonous Autralian lake. Nature 18 (1878) 11-12
8. Groten J.P., Feron V.J., and Sühnel J. Toxicology of simple and complex mixtures. Trends Pharmacol. Sci. 22 (2001) 316-322
9. Hisbergues M., Christiansen G., Rouhiainen L., Sivonen K., and Börner T. PCR-based identification of microcystin-producing genotypes of different cyanobacterial genera. Arch. Microbiol. 180 (2003) 402-410
10. Jungblut A.-D., and Neilan B.A. Molecular identification and evolution of the cyclic peptide hepatotoxins, microcystin and nodularin, synthetase genes in three orders of cyanobacteria. Arch. Microbiol. 185 (2006) 107-114
11. Kankaanpää H., Sipiä V., Kuparinen J., Chizmar J., and Carmichael W.W. Nodularin analyses and toxicity of Nodularia spumigena (Nostocales, Cyanobacteria) waterbloom in the western Gulf of Finland, Baltic Sea, in August 1999. Phycologia 40 (2001) 268-274
12. Kiviranta J., Sivonen K., Niemelà S.I., and Huovinen K. Detection of toxicity of cyanobacteria by Artemia salina bioassay. Environ. Toxicol. Water Qual. 6 (1991) 423-436
13. Komárek J., and Anagnostidis K. Cyanoprokaryota, Part 2, Oscillatoriales, Suüsswasserflora von Mitteleuropa (2005), Spektrum Akademischer Verlag, Heidelberg, Bd 19/2 pp. 1-759
14. Komarék J., Hübel M., Hübel H., and Śmarda J. The Nodularia studies 2 tax. Algol. Stud. 68 (1993) 1-25
15. Komarék J., Kling H., and Komárková J. Filamentous cyanobacteria. In: Wehr J.D., and Sheath R.G. (Eds). Freshwater Algae of North America (2003), Academic Press, San Diego 177-196
16. Kotai J. Instructions for Preparation of Modified Nutrient Solution Z8 for Algae (1972), Norwegian Institute for Water Research, Oslo, Blindern p. 5
17. Laamanen M.J., Gugger M.F., Lehtimäki J.M., Haurakka K., and Sivonen K. Diversity of toxic and nontoxic nodularia isolates (Cyanobacteria) and filaments from the Baltic Sea. Appl. Environ. Microbiol. 67 (2001) 4638-4647
18. Lehtimäki J., Lyra C., Suomalainen S., Sundman P., Rouhiainen L., Paulin L., Salkinoja-Salonen M., and Sivonen K. Characterization of Nodularia strains, cyanobacteria from brackish waters, by genotypic and phenotypic methods. Int. J. Syst. Evol. Microbiol. 3 (2000) 1043-1053
19. Lyra C., Laamanen M., Lehtimäki J.M., Surakka A., and Sivonen K. Benthic cyanobacteria of the genus Nodularia are non-toxic, without gas vacuoles, able to glide and genetically more diverse than planktonic Nodularia. Int. J. Syst. Evol. Microbiol. 55 (2005) 555-568
20. Martins R., Fernandez N., Beiras R., and Vasconcelos V. Toxicity assessment of crude and partially purified extracts of marine Synechocystis and Synechococcus cyanobacterial strains in marine invertebrates. Toxicon 50 (2007) 791-799
21. Mersch-Sundermann V., Mochayedi S., and Kevekordes S. Genotoxicity of polycyclic aromatic hydrocarbons in Escherichia coli PQ37. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 278 (1992) 1-9
22. Moffitt M.C., and Neilan B.A. On the presence of peptide synthetase and polyketide synthase genes in the cyanobacterial genus Nodularia. FEMS Microbiol. Lett. 196 (2001) 207-214
23. Mortelmans K., and Zeiger E. The Ames Salmonella/microsome mutagenicity assay. Mutat. Res. 455 (2000) 29-60
24. Neilan B.A., Jacobs D., Del Dot T., Blackall L.L., Hawkins P.R., Cox P.T., and Goodman A.E. rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus Microcystis. Int. J. Syst. Bacteriol. 47 (1997) 693-697
25. Pearson L.A., Moffitt M.C., Ginn H.P., and Neilan B.A. The molecular genetics and regulation of cyanobacterial peptide hepatotoxin biosynthesis. Crit. Rev. Toxicol. 38 (2008) 847-856
26. Posada D., and Crandall K.A. MODELTEST: testing the model of DNA substitution. Bioinformatics 14 (1998) 817-818
27. Pushparaj B., Pelosi E., and Caroppo S. Effect of Nodularia harveyana biomass on the incidence of root-knot nematode (Meloidogyne incognita) in tomato. J. Appl. Phycol. 12 (2000) 489-492 489-492
28. Pushparaj B., Pelosi E., and Jüttner F. Toxicological analysis of the marine cyanobacterium Nodularia harveyana. J. Appl. Phycol. 10 (1999) 527-530
29. Ronquist F., and Huelsenbeck J.P. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (2003) 1572-1574
30. Ruiz M.J., and Marzin D. Genotoxicity of six pesticides by Salmonella mutagenicity test and SOS chromotest. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 390 (1997) 245-255
31. Saito K., Konno A., Ishii H., Saito H., Nishida F., Abe T., and Chen C. Nodularin-Har: a new nodularin from Nodularia. J. Nat. Prod. 64 (2001) 139-141
32. Sánches-Fortún S., Sanz S., and Barahona M.V. Acute toxicity of several organophosphorous insecticides and protection by cholinergic antagonist and 2-PAM on Artemia salina larvae. Arch. Environ. Contam. Toxicol. 31 (1996) 391-398
33. Sivonen K., Kononen W., Carmichael W.W., Dahlem A.M., Rinehart K.L., Kiviranta J., and Niemela S.I. Occurrence of the hepatotoxic cyanobacterium Nodularia spumigena in the Baltic Sea and structure of the toxin. Appl. Environ. Microbiol. 55 (1989) 1990-1995
34. StatSoft, Inc. STATISTICA (Data Analysis Software System), Version 7 (2004)
35. Swofford D.L. PAUP4.0b10 Phylogenetic Analysis Using Parsimony and Other Methods' Computer Program (2001), Sinauer, Sunderland, MA
36. Tamura K., Dudley J., Nei M., and Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24 (2007) 1596-1599
37. Welker M., Fastner J., Erhard M., and Von Döhren H. Application of MALDI-TOF MS in cyanotoxin research. Environ. Toxicol. 17 (2002) 367-374
38. Wiegand C., and Pflugmacher S. Ecotoxicological effects of selected cyanobacterial secondary metabolites: a short review. Toxicol. Appl. Pharmacol. 203 (2005) 201-218
39. Wilmotte A. Molecular evolution and taxonomy of the cyanobacteria. In: Bryant D.A. (Ed). The Molecular Biology of Cyanobacteria (1994), Kluwer, Dordrecht pp. 1-25