Quantification of mutated alleles of 23S rRNA in macrolide-resistant campylobacter by taqMan real-time polymerase chain reaction

Foodborne Pathogens and Disease

Volumn 7, Issue 1, 2010, Pages 43-49

  Hao, Haihong ^a   Dai, Menghong ^a   Wang, Yulian ^a   Chen, Dongmei ^a   Yuan, Zonghui ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ERYTHROMYCIN ETHYLSUCCINATE; RNA 23S; TYLOSIN;

ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; CAMPYLOBACTER; CONTROLLED STUDY; DNA SEQUENCE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHARMACOGENETICS; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WILD TYPE;

ALLELES; ANIMALS; ANTI-BACTERIAL AGENTS; CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; CHICKENS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENE DOSAGE; GENES, BACTERIAL; GENOTYPE; MACROLIDES; MICROBIAL SENSITIVITY TESTS; PHENOTYPE; POINT MUTATION; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 23S; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

CAMPYLOBACTER;

EID: 74549172078     PISSN: 15353141     EISSN: None     Source Type: Journal    
DOI: 10.1089/fpd.2009.0339     Document Type: Article

References (21)

1. Alonso R, Mateo E, Churruca E, Martinez I, Girbau C, and Fernandez-Astorga A. MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains. J Microbiol Methods 2005;63:99-103.
2. Altekruse SF, Stern NJ, Fields PI, and Swerdlow DL. Campylobacter jejuni-an emerging foodborne pathogen. Emerg Infect Dis 1999;5:28-35.
3. Blaser MJ. Epidemiologic and clinical features of Campylobacter jejuni infections. J Infect Dis 1997;176 Suppl 2:S103-S105.
4. Blaser MJ, Taylor DN, and Feldman RA. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev 1983;5:157-176.
5. Caldwell DB, Wang Y, and Lin J. Development, stability, and molecular mechanisms of macrolide resistance in Campylobacter jejuni. Antimicrob Agents Chemother 2008;52:3947-3954.
6. CLSI [Clinical and Laboratory Standards Institute]. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrenquently Isolated or Fastidious Bacteria; Approved Guideline Vol. CLSI Document M45-A. Wayne, PA: Clinical and Laboratory Standards Institute 2006.
7. Engberg J, Aarestrup FM, Taylor DE, Gerner-Smidt P, and Nachamkin I. Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis 2001;7:24-34.
8. Gibreel A, Kos VN, Keelan M, Trieber CA, Levesque S, Michaud S, and Taylor DE. Macrolide resistance in Campylobacter jejuni and Campylobacter coli: molecular mechanism and stability of the resistance phenotype. Antimicrob Agents Chemother 2005;49:2753-2759.
9. Gibson UE, Heid CA, and Williams PM. A novel method for real time quantitative RT-PCR. Genome Res 1996;6:995-1001.
10. Gupta A, Nelson JM, Barrett TJ, et al. Antimicrobial resistance among Campylobacter strains, United States, 1997-2001. Emerg Infect Dis 2004;10:1102-1109.
11. Jensen LB and Aarestrup FM. Macrolide resistance in Campylobacter coli of animal origin in Denmark. Antimicrob Agents Chemother 2001;45:371-372.
12. Ladely SR, Harrison MA, Fedorka-Cray PJ, Berrang ME, Englen MD, and Meinersmann RJ. Development of macrolideresistant Campylobacter in broilers administered subtherapeutic or therapeutic concentrations of tylosin. J Food Prot 2007;70:1945-1951.
13. Ladely SR, Meinersmann RJ, Englen MD, Fedorka-Cray PJ, and Harrison MA. 23S rRNA gene mutations contributing to macrolide resistance in Campylobacter jejuni and Campylobacter coli. Foodborne Pathog Dis 2009;6:91-98.
14. Livak KJ, Flood SJ, Marmaro J, Giusti W, and Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. Genome Res 1995;4:357-362.
15. Niwa H, Chuma T, Okamoto K, and Itoh K. Rapid detection of mutations associated with resistance to erythromycin in Campylobacter jejuni=coli by PCR and line probe assay. Int J Antimicrob Agents 2001;18:359-364.
16. Niwa H, Chuma T, Okamoto K, and Itoh K. Simultaneous detection of mutations associated with resistance to macrolides and quinolones in Campylobacter jejuni and C. coli using a PCRline probe assay. Int J Antimicrob Agents 2003;22:374-379.
17. Vacher S, Menard A, Bernard E, and Megraud F. PCR-restriction fragment length polymorphism analysis for detection of point mutations associated with macrolide resistance in Campylobacter spp. Antimicrob Agents Chemother 2003;47:1125-1128.
18. Vacher S, Menard A, Bernard E, Santos A, and Megraud F. Detection of mutations associated with macrolide resistance in thermophilic Campylobacter spp. by real-time PCR. Microb Drug Resist 2005;11:40-47.
19. Van Looveren M, Daube G, De Zutter L, et al. Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium. J Antimicrob Chemother 2001;48:235-240.
20. Volkmann H, Schwartz T, Bischoff P, Kirchen S, and Obst U. Detection of clinically relevant antibiotic-resistance genes in municipal wastewater using real-time PCR (TaqMan). J Microbiol Methods 2004;56:277-286.
21. Whelan JA, Russell NB, and Whelan MA. A method for the absolute quantification of cDNA using real-time PCR. J Immunol Methods 2003;278:261-269.