Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

Medicina Oral, Patologia Oral y Cirugia Bucal

Volumn 15, Issue 1, 2010, Pages

  Navarro Escobar, Esther ^a   González Rodríguez, María Paloma ^a   Ferrer Luque, Carmen María ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

Citric acid; Cytotoxicity; Phosphoric acid; Root canal irrigants; Sodium hypochlorite

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CITRIC ACID; HYPOCHLORITE SODIUM; PHOSPHORIC ACID;

ANIMAL CELL; ARTICLE; CELL VIABILITY; COLORIMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DILUTION; DRUG CYTOTOXICITY; EMBRYO; ENDODONTICS; FIBROBLAST CULTURE; LAVAGE; MOUSE; NONHUMAN;

CELLS, CULTURED; CITRIC ACID; CYTOTOXINS; DENTAL MATERIALS; FIBROBLASTS; PHOSPHORIC ACIDS; ROOT CANAL THERAPY; SODIUM HYPOCHLORITE;

EID: 74549158271     PISSN: 16984447     EISSN: 16986946     Source Type: Journal    
DOI: 10.4317/medoral.15.e90     Document Type: Article

References (20)

1. Zhang W, Torabinejad M, Li Y. Evaluation of cytotoxicity of MTAD using the MTT-tetrazolium method. J Endod. 2003;29:654-7.
2. Hidalgo E, Bartolome R, Dominguez C. Cytotoxicity mechanisms of sodium hypochlorite in cultured human dermal fibroblasts and its bactericidal effectiveness. Chem Biol Interact. 2002;139:265-82.
3. Garberoglio R, Becce C. Smear layer removal by root canal irrigants. A comparative scanning electron microscopic study. Oral Surg Oral Med Oral Pathol. 1994;78:359-67.
4. Pérez-Heredia M, Ferrer-Luque CM, González-Rodríguez MP. The effectiveness of different acid irrigating solutions in root canal cleaning after hand and rotary instrumentation. J Endod. 2006;32:993-7.
5. Siqueira JF Jr, Rôças IN, Favieri A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod. 2000;26:331-4.
6. Zehnder M. Root canal irrigants. J Endod. 2006;32:389-98.
7. Carson KR, Goodell GG, McClanahan SB. Comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens. J Endod. 2005;31:471-3.
8. Pashley EL, Birdsong NL, Bowman K, Pashley DH. Cytotoxic effects of NaOCl on vital tissue. J Endod. 1985;11:525-8.
9. Serper A, Calt S, Dogan AL, Guc D, Ozçelik B, Kuraner T. Comparison of the cytotoxic effects and smear layer removing capacity of oxidative potential water, NaOCl and EDTA. J Oral Sci. 2001;43:233-8.
10. Scelza MF, Antoniazzi JH, Scelza P. Efficacy of final irrigation - a scanning electron microscopic evaluation. J Endod. 2000;26:355-8.
11. Malheiros CF, Marques MM, Gavini G. In vitro evaluation of the cytotoxic effects of acid solutions used as canal irrigants. J Endod. 2005;31:746-8.
12. Sousa SM, Bramante CM, Taga EM. Biocompatibility of EDTA, EGTA and citric acid. Braz Dent J. 2005;16:3-8.
13. Amaral KF, Rogero MM, Fock RA, Borelli P, Gavini G. Cytotoxicity analysis of EDTA and citric acid applied on murine resident macrophages culture. Int Endod J. 2007;40:338-43.
14. Chan CP, Jeng JH, Hsieh CC, Lin CL, Lei D, Chang MC. Morphological alterations associated with the cytotoxic and cytostatic effects of citric acid on cultured human dental pulp cells. J Endod. 1999;25:354-8.
15. Ayad MF. Effects of rotary instrumentation and different etchants on removal of smear layer on human dentin. J Prosthet Dent. 2001;85:67-72.
16. Pérez-Heredia M, Ferrer-Luque CM, González-Rodríguez MP, Martín-Peinado FJ, González-López S. Decalcifying effect of 15% EDTA, 15% citric acid, 5% phosphoric acid and 2.5% sodium hypochlorite on root canal dentine. Int Endod J. 2008;41:418-23.
17. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63.
18. Wall GL, Dowson J, Shipman C Jr. Antibacterial efficacy and cytotoxicity of three endodontic drugs. Oral Surg Oral Med Oral Pathol. 1972;33:230-41.
19. Chang YC, Huang FM, Tai KW, Chou MY. The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;92:446-50.
20. Goldman SA, Pulsinelli WA, Clarke WY, Kraig RP, Plum F. The effects of extracellular acidosis on neurons and glia in vitro. J Cereb Blood Flow Metab. 1989;9:471-7.