Cytotoxicity analysis of EDTA and citric acid applied on murine resident macrophages culture

International Endodontic Journal

Volumn 40, Issue 5, 2007, Pages 338-343

  Amaral, K F ^a   Rogero, M M ^a   Fock, R A ^a   Borelli, P ^a   Gavini, G ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cell culture; Citric acid; Cytotoxicity; EDTA; Macrophages; Root canal irrigants

Indexed keywords

BIOMEDICAL AND DENTAL MATERIALS; CITRIC ACID; EDETIC ACID;

ANIMAL; ARTICLE; DRUG EFFECT; MACROPHAGE; MALE; MOUSE; TIME;

ANIMALS; CITRIC ACID; EDETIC ACID; MACROPHAGES; MALE; MICE; ROOT CANAL IRRIGANTS; TIME FACTORS;

EID: 34247358261     PISSN: 01432885     EISSN: 13652591     Source Type: Journal    
DOI: 10.1111/j.1365-2591.2007.01220.x     Document Type: Article

References (19)

1. Al-Omari MAO, Dummer PMH (1995) Canal blockage and debris extrusion with eight preparation techniques. Journal of Endodontics 21, 154 8.
2. Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza-Fill FJ (2001) Apical extrusion of debris and irrigants using two hand and three engine-driven instrumentation techniques. International Endodontic Journal 34, 354 8.
3. Freshney RI (1990) Measurement of cytotoxicity and viability. In : Freshney RI, ed. Culture of Animal Cells: A Manual of Basic Technique, 2nd edn. New York, USA : Willy-Liss, pp. 7 13.
4. Garberoglio R, Becce C (1994) Smear layer removal by root canal irrigants. Oral Surgery, Oral Medicine, Oral Pathology 78, 359 67.
5. Hülsmann M, Heckendorff M, Lennon Á (2003) Chelating agents in root canal treatment: mode of action and indications for their use. International Endodontic Journal 36, 810 30.
6. Kawahara H, Yamagami A, Nakamura M (1968) Biological testing of dental materials by means of tissue culture. International Dental Journal 9, 443 67.
7. Kopp W, Schwarting R (1989) Differentiation of T lymphocyte subpopulations, macrophages, and HLA-DR-restricted cells of apical granulation tissue. Journal of Endodontics 15, 72 5.
8. Malheiros CF, Gavini G (1998) Demineralizing effect of citric acid in different concentrations over different periods of time. Revista da Pós-Graduação da Faculdade de Odontologia da Universidade de São Paulo 5, 64 8.
9. Malheiros CF, Marques MM, Gavini G (2005) In vitro evaluation of the cytotoxic effects of acid solutions used as canal irrigants. Journal of Endodontics 31, 746 8.
10. Metzger Z (2000) Macrophages in periapical lesions. Dental Traumatology 16, 1 8.
11. Piatelli A, Artese L, Rosini S, Quaranta M, Musiani P (1991) Immune cells in periapical granuloma: morphological and immunohistochemical characterization. Journal of Endodontics 17, 26 9.
12. Scelza MFZ, Antoniazzi JH, Scelza P (2000) Efficacy of final irrigation - a scanning electron microscopic evaluation. Journal of Endodontics 26, 355 8.
13. Scelza MF, Pinheiro Daniel RLD, Santos EM, Jaeger MMM (2001) Cytotoxic effects of 10% citric acid and EDTA-T used as root canal irrigants: an in vitro analysis. Journal of Endodontics 27, 741 3.
14. Schmalz G (1994) Use of cell cultures for toxicity testing of dental materials - advantages and limitations. Journal of Dentistry 2, 6 11.
15. Segura JJ, Calvo JR, Guerrero JM, Sampedro C, Jimenez A, Llamas R (1996) The disodium salt of EDTA inhibits the binding of vasoactive intestinal peptide to macrophage membranes: endodontic implications. Journal of Endodontics 22, 337 40.
16. Segura JJ, Calvo JR, Guerrero JM, Jimenez-Planas A, Sampedro C, Llamas R (1997) EDTA inhibits in vitro substrate adherence capacity of macrophages: endodontic implications. Journal of Endodontics 23, 205 8.
17. Segura-Egea JJ, Jiménez-Rubio A, Rios-Santos JV, Velasco-Ortega E, Calvo-Gutierrez JR (2003) In vitro inhibitory effect of EGTA on macrophage adhesion: endodontic implications. Journal of Endodontics 29, 211 3.
18. Teixeira CS, Felippe MCS, Felippe WT (2005) The effect of application time of EDTA and NaOCl on intracanal smear layer removal: an SEM analysis. International Endodontic Journal 38, 285 90.
19. Yamaguchi M, Yoshida K, Suzuki R, Nakamura H (1996) Root canal irrigation with citric acid solution. Journal of Endodontics 22, 27 9.