Design, expression and characterization of mutants of fasciculin optimized for interaction with its target, acetylcholinesterase

Protein Engineering, Design and Selection

Volumn 22, Issue 10, 2009, Pages 641-648

  Sharabi, Oz ^a   Peleg, Yoav ^b   Mashiach, Efrat ^a,c   Vardy, Eyal ^a,d   Ashani, Yacov ^b   Simian, Israel ^b   Sussman, Joel L ^b   Shifman, Julia M ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c TEL AVIV UNIVERSITY   (Israel)

^d DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Acetylcholinesterase binding affinity computational protein design fasciculin protein protein interactions

Indexed keywords

ACETYLCHOLINESTERASE; EFFICIENT STRATEGY; FREE ENERGY OF BINDING; MOLECULAR CONTACTS; MOLECULAR ENERGIES; NATIVE CONFORMATION; PROTEIN AFFINITY; PROTEIN INTERACTION; PROTEIN-PROTEIN COMPLEXES; REFOLDING; SINGLE MUTATION; TOTAL ENERGY; WILD TYPES;

ENZYMES; ESCHERICHIA COLI; MOLECULAR STRUCTURE; TOXIC MATERIALS;

COMPLEXATION;

ACETYLCHOLINESTERASE; FASCICULIN; SNAKE VENOM;

AMINO ACID SEQUENCE; ARTICLE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PROTEIN INTERACTION; TORPEDO CALIFORNICA; WILD TYPE;

ACETYLCHOLINESTERASE; AMINO ACID SEQUENCE; ELAPID VENOMS; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECOMBINANT PROTEINS; THERMODYNAMICS;

ESCHERICHIA COLI; TORPEDO CALIFORNICA;

EID: 74549123515     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp045     Document Type: Article

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