Label-free fluorescent real-time monitoring of adenylyl cyclase

Bioorganic and Medicinal Chemistry Letters

Volumn 20, Issue 3, 2010, Pages 1145-1147

  Rhee, Hyun Woo ^a   Kim, Kyoung Shim ^b   Han, Pyung Lim ^b   Hong, Jong In ^a  

^a Seoul National University   (South Korea)

^b Ewha Womans University   (South Korea)

Author keywords

Adenylyl cyclase; Cyclic AMP; Fluorescent chemosensor; Pyrophosphate; Real time monitoring

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENYLATE CYCLASE; CYCLIC AMP; PYROPHOSPHATE; (1-((PYREN-1-YL)PROPOXY)-3,5-BIS(BIS(2-PYRIDYLMETHYL)AMINOMETHYL)BENZENE)ZINC(II); FLUORESCENT DYE; GUANOSINE TRIPHOSPHATE; PYRENE DERIVATIVE; PYRIDINE DERIVATIVE;

ARTICLE; CHEMICAL BINDING; CHEMICAL STRUCTURE; CHEMOSENSITIZATION; FLUORESCENCE; HYDROLYSIS; SYNTHETIC FLUORESCENT CHEMOSENSOR; ANIMAL; BRAIN; CHEMISTRY; COMPARATIVE STUDY; COMPUTER SYSTEM; METABOLISM; MOUSE; PROCEDURES; SPECTROFLUOROMETRY;

ADENOSINE TRIPHOSPHATE; ADENYLATE CYCLASE; ANIMALS; BRAIN; COMPUTER SYSTEMS; FLUORESCENT DYES; GUANOSINE TRIPHOSPHATE; MICE; PYRENES; PYRIDINES; SPECTROMETRY, FLUORESCENCE;

EID: 74049099138     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.12.018     Document Type: Article

References (26)

1. Robison G.A., Butcher R.W., and Sutherland E.W. Annu. Rev. Biochem. 37 (1968) 149
2. Sunahara R.K., Dessauer C.W., and Gilman A.G. Annu. Rev. Pharmacol. Toxicol. 36 (1996) 461
3. Atkins F.L., Dowell R.T., and Love S.J. Cardiovasc. Pharmacol. 7 (1985) 66
4. Bonkale W.L., Fastbom J., Wiehager B., Ravid R., Winblad B., and Cowburn R.F. Brain Res. 737 (1996) 155
5. Salomon Y., Londos C., and Rodbell M. Anal. Biochem. 58 (1974) 541
6. Cunliffe J.M., Sunahara R.K., and Kennedy R.T. Anal. Chem. 78 (2006) 1731
7. Lee K.W., Hong J.H., Choi I.Y., Che Y., Lee J.K., Yang S.D., Song C.W., Kang H.S., Lee J.H., and Noh J.S. J. Neurosci. 22 (2002) 7931
8. Kim K.S., Lee K.W., Im J.Y., Yoo J.Y., Kim S.W., Lee J.K., Nestler E.J., and Han P.L. Proc. Natl. Acad. Sci. U.S.A. 103 (2006) 3908
9. Lee D.H., Im J.H., Son S.U., Chung Y.K., and Hong J.I. J. Am. Chem. Soc. 125 (2003) 7752
10. Lee D.H., Kim S.Y., and Hong J.I. Angew. Chem., Int. Ed. 43 (2004) 4777
11. Cho H.K., Lee D.H., and Hong J.I. Chem. Commun. (2005) 1690
12. Kim S.K., Lee D.H., Hong J.-I., and Yoon J. Acc. Chem. Res. 42 (2009) 23
13. Ojida A., Takashima I., Kohira T., Nonaka H., and Hamachi I. J. Am. Chem. Soc. 130 (2008) 12095
14. Ojida A., Nonaka H., Miyahara Y., Tamaru S., Sada K., and Hamachi I. Angew. Chem., Int. Ed. 45 (2006) 5518
15. Sakamoto T., Ojida A., and Hamachi I. Chem. Commun. (2009) 141
16. Ojida A., Park S.-k., Mito-oka Y., and Hamachi I. Tetrahedron Lett. 43 (2002) 6193
17. Leevy W.M., Gammon S.T., Jiang H., Johnson J.R., Maxwell D.J., Jackson E.N., Marquez M., Piwnica-Worms D., and Smith B.D. J. Am. Chem. Soc. 128 (2006) 16476
18. Jiang H., O'neil E.J., Divittorio K.M., and Smith B.D. Org. Lett. 7 (2005) 3013
19. Rhee H.W., Choi H.Y., Han K., and Hong J.-I. J. Am. Chem. Soc. 129 (2007) 4524
20. Rhee H.W., Lee C.R., Cho S.H., Song M.R., Cashel M., Choy H.E., Seok Y.J., and Hong J.-I. J. Am. Chem. Soc. 130 (2008) 784
21. Rhee H.W., Choi S.J., Yoo S.H., Jang Y.O., Park H.H., Pinto R.M., Cameselle J.C., Sandoval F.J., Roje S., Han K., Chung D.S., Suh J., and Hong J.-I. J. Am. Chem. Soc. 131 (2009) 10107
22. Itoh H., Ishido S., Nomura M., Hayakawa T., and Mitaku S. J. Phys. Chem. 100 (1996) 9047
23. 4, 1 mM EGTA, 30 mM NaCl, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 0.5 mM dithiothreitol (DTT), 1.8, 4.5 or 9.0 μg AC (V) complex. Total reaction mixture volume = 100 μl.
24. Braun S., and Levitzki A. Biochemistry 18 (1979) 2134
25. Tolkovsky A.M., Braun S., and Levitzki A. Proc. Natl. Acad. Sci. U.S.A. 79 (1982) 213
26. Richards J.M., Tierneyl J.M., and Swislocki N.I. J. Biol. Chem. 256 (1981) 8889