Expression of the hGM-CSF in the silk glands of germline of gene-targeted silkworm

Biochemical and Biophysical Research Communications

Volumn 391, Issue 3, 2010, Pages 1427-1431

  Li, Yanmei ^a   Cao, Guangli ^a   Chen, Huimei ^a   Jia, Haifang ^a   Xue, Renyu ^a   Gong, Chengliang ^a  

^a SOOCHOW UNIVERSITY   (China)

Author keywords

Bombyx mori; Gene targeting; hGM CSF; Silk gland; Transformation silkworm

Indexed keywords

ANTIBODY; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; GREEN FLUORESCENT PROTEIN; SILK FIBROIN;

ARTICLE; CONTROLLED STUDY; DOT HYBRIDIZATION; EXON; GENE EXPRESSION REGULATION; GENE SEQUENCE; GENE TARGETING; GENE TRANSFER; GENE VECTOR; HEAVY CHAIN; INSECT GENOME; INTRON; LIGHT CHAIN; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; SILK; SILKWORM; SPERM; WESTERN BLOTTING;

ANIMALS; BOMBYX; CLONING, MOLECULAR; FIBROINS; GENE TARGETING; GENETIC VECTORS; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; GREEN FLUORESCENT PROTEINS; HUMANS; MALE; OVUM; SPERMATOZOA; TRANSFORMATION, GENETIC;

BOMBYX MORI;

EID: 73949157034     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.12.082     Document Type: Article

References (32)

1. Tanaka K., Kajiyama N., Ishikura K., Waga S., Kikuchi A., Ohtomo K., Takagi T., and Mizuno S. Determination of the site of disulfide linkage between heavy and light chains of silk fibroin produced Bombyx mori. Biochim. Biophys. Acta 1432 (1999) 92-103
2. Kurihara H., Sezutsu H., Tamura T., and Yamada K. Production of an active feline interferon in the cocoon of transgenic silkworms using the fibroin H-chain expression system. Biochem. Biophys. Res. Commun. 355 (2007) 976-980
3. Tamura T., Thibert C., Royer C., Kanda T., Abraham E., Kamba M., Komoto N., Thomas J.L., Mauchamp B., Chavancy G., Shirk P., Fraser M., Prudhomme J.C., and Couble P. Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon derived vector. Nat. Biotechnol. 18 (2000) 81-84
4. Ogawa S., Tomita M., Shimizu K., and Yoshizato K. Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: Production of recombinant human serum albumin. J. Biotechnol. 128 (2007) 531-544
5. Tomita M., Munetsuna H., Sato T., Adachi T., Hino R., Hayashi M., Shimizu K., Nakamura N., Tamura T., and Yoshizato K. Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat. Biotechnol. 21 (2003) 52-56
6. Hino R., Tomita M., and Yoshizato K. The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor. Biomaterials 27 (2006) 5715-5724
7. Fraser M.J., Ciszczon T., Elick T., and Bauser C. Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol. Biol. 5 (1996) 141-151
8. Elick T.A., Bauser C.A., and Fraser M.J. Excision of the piggyBac transposable element in vitro is a precise event that is enhanced by the expression of its encoded transposase. Genetica 98 (1996) 33-41
9. Wu X.F., and Cao C.P. Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination. J. Zhejiang Univ. Sci. 5 (2004) 644-650
10. Thompson S., Clarke A.R., Pow A.M., Hooper M.L., and Melton D.W. Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Cell 56 (1989) 313-321
11. McCreath K.J., Howcroft J., Campbell K.H.S., Colman A., Schnieke A.E., and Kind A.J. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405 (2000) 1066-1069
12. Yamao M., Katayama N., Nakazawa H., Yamakawa M., Hayashi Y., Hara S., Kamei K., and Mori H. Gene targeting in the silkworm by use of a baculovirus. Commun. Res. 13 (1999) 511-516
13. Metcalf D. The granulocyte-macrophage colony-stimulating factors. Cell 43 (1985) 5-6
14. Chen J., Wu X.F., and Zhang Y.Z. Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor. Biotechnology 123 (2006) 236-247
15. Burgess A.W., Begley C.G., Johnson G.R., Lopez A.F., Williamson D.J., Mermod J.J., Simpson R.J., Schmitz A., and DeLamarter J.F. Purification and properties of bacterially synthesized human granulocyte-macrophage colony-stimulating factor. Blood 69 (1987) 43-51
16. Miyajima A., Otsu K., Schreurs J., Bond M.W., Abrams J.S., and Arai K. Expression of murine and human granulocyte-macrophage colony-stimulating factors in S. cerevisiae: mutagenesis of the potential glycosylation sites. EMBO J. 5 (1986) 1193-1197
17. Sardana R.K., Alli Z., Dudani A., Tackaberry E., Panahi M., Narayanan M., Ganz P., and Altosaar I. Biological activity of human granulocyte-macrophage colony stimulating factor is maintained in a fusion with seed glutelin peptide. Transgenic Res. 11 (2002) 521-531
18. Shi X., Qin J., Zhu J., and Zhu D. Expression of biologically active human granulocyte-macrophage colon-stimulating factor in the silkworm (Bombyx mori). Biotechnol. Appl. 2 (1996) 245-249
19. Ryoo Z.Y., Kim M.O., Kim K.E., Bahk Y.Y., Lee J.W., Park S.H., HoiKim J., Byun S.J., Hwang H.Y., Youn J., and Kim T.Y. Expression of recombinant human granulocyte macrophage-colony stimulating factor (hGM-CSF) in mouse urine. Transgenic Res. 1 (2001) 193-200
20. Kodaira K., Takahashi R., Hirabayashi M., Suzuki T., and Ueda M. Recombinant human GM-CSF in the milk of transgenic rats. Theriogenology 47 (1997) 216
21. Zhang W., Lv Z., Nie Z., Chen G.G., Chen J., Sheng Q., Yu W., Jin Y.F., Wu X.G., and Zhang Y.Z. Bioavailability of orally administered rhGM-CSF: a single-dose, randomized, open-label, two-period crossover trial. PLoS ONE 5 (2009) 5353
22. Hara W., Kosegawa E., Mase K., and Kadono-Okuda K. Improvement of linkage analysis in the silkworm, Bombyx mori, by using cDNA clones' RFLP. J. Sericult. Sci. Jpn. 71 (2002) 95-100 (in Japanese)
23. Cao G.L., Xue R.Y., He Z., Zheng X.J., Shen W.D., and Gong C.L. Research on hGM-CSF Transgenic Silkworm With piggyBac transposon. Can Ye Ke Xue 32 (2006) 324-327
24. Zhao Y., Li X., Cao G.L., Xue R.Y., and Gong C.L. The expression of hIGF-I protein in silk gland of transgenic silkworm and transformed cells from silkwom. Sci. China C Life Sci. 39 (2009) 677-684
25. Guo X.Y., Zhou Z.Y., Feng L.C., Wang L., Lu C., and Xiang Z.H. Sperm-mediated gene transformation of silkworm. Prog. Biochem. Biophys. 28 (2001) 423-425 (in Chinese)
26. Zhang F., Zhao Y., and Lu C.D. Fluorescent transgenic silkworm. Acta Biochim. Biophys. Sin. (Shanghai) 31 (1999) 119-123
27. Inoue S., Tanaka K., Arisaka F., Kimura S., Ohtomo K., and Mizuno S. Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6:6:1 molar ratio. J. Biol. Chem. 275 (2000) 40517-40528
28. Takei F., Kikuchi Y., Kikuchi A., Mizuno S., and Shimura K. Further evidence for importance of the subunit combination of silk fibroin in its efficient secretion from the posterior silk gland cells. J. Cell Biol. 105 (1987) 175-180
29. Mori K., Tanaka K., Kikuchi Y., Waga M., Waga S., and Mizuno S. Production of a chimeric fibroin light-chain polypeptide in a fibroin secretion-deficient naked pupa mutant of the silkworm Bombyx mori. J. Mol. Biol. 251 (1995) 217-228
30. Gong C.L., Xue R.Y., Cao G.L., Shi X.Y., and Shen W.D. HGM-CSF expression by using recombinant BmNPV with inactivated chitinase and cathepsin gene. Prog. Inorg. Biochem. Biophys. 32 (2005) 947-952
31. Adachi T., Tomita M., Shimizu K., Ogawa S., and Yoshizato K. Generation of hybrid transgenic silkworms that express Bombyx mori prolyl-hydroxylase α-subunits and human collagens in posterior silk glands: Production of cocoons that contained collagens with hydroxylated praline residues. J. Biotechnol. 126 (2006) 205-219
32. Zhu Y.X., Li Y., and Zheng X.F. Modern Molecular Biology, The Expression and Regulation of Gene (Down Volume) - The General Principle of Expression and Regulation Over Eukaryotic Genes (2002), Higher Education Press, Beijing p. 288