Comparison of flow cytometry and immunohistochemistry in non-radioisotopic murine lymph node assay using bromodeoxyuridine

Toxicology Letters

Volumn 192, Issue 2, 2010, Pages 229-237

  Jung, Kyoung Mi ^a   Bae, Il Hong ^a   Kim, Bae Hwan ^b   Kim, Wang Ki ^a   Chung, Jin Ho ^c   Park, Young Ho ^a   Lim, Kyung Min ^a  

^a AmorePacific Corporation   (South Korea)

^b Keimyung University   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

5 Bromo 2 deoxyuridine; Flow cytometry; Immunohistochemistry; Non radioisotopic local lymph node assay

Indexed keywords

1 CHLORO 2,4 DINITROBENZENE; 2 PROPANOL; BROXURIDINE; CINNAMALDEHYDE DERIVATIVE; DODECYL SULFATE SODIUM; EUGENOL; HEXYLCINNAMALDEHYDE; ISOEUGENOL; PHENYLENEDIAMINE; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EAR DISEASE; EDEMA; FEMALE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HISTOPATHOLOGY; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; LYMPH NODE; LYMPH NODE CELL; LYMPHATIC SYSTEM EXAMINATION; MOUSE; NONHUMAN; ORGAN WEIGHT; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SENSITIZATION;

ANIMALS; BROMODEOXYURIDINE; CELL COUNT; EAR; FEMALE; FLOW CYTOMETRY; IMMUNOHISTOCHEMISTRY; LOCAL LYMPH NODE ASSAY; LYMPH NODES; MICE; MICE, INBRED BALB C; ORGAN SIZE;

MURINAE; MUS;

EID: 73749084093     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2009.10.024     Document Type: Article

References (22)

1. Basketter D.A., Miettinen J., and Lahti A. Acute irritant reactivity to sodium lauryl sulfate in atopics and non-atopics. Contact Dermat. 38 (1998) 253-257
2. Betts C.J., Dearman R.J., Heylings J.R., Kimber I., and Basketter D.A. Skin sensitization potency of methyl methacrylate in the local lymph node assay: comparisons with guinea-pig data and human experience. Contact Dermat. 55 (2006) 140-147
3. Boussiquet-Leroux C., Durand-Cavagna G., Herlin K., and Holder D. Evaluation of lymphocyte proliferation by immunohistochemistry in the local lymph node assay. J. Appl. Toxicol. 15 (1995) 465-475
4. Dearman R.J., Scholes E.W., Ramdin L.S., Basketter D.A., and Kimber I. The local lymph node assay: an interlaboratory evaluation of interleukin 6 (IL-6) production by draining lymph node cells. J. Appl. Toxicol. 14 (1994) 287-291
5. Dearman R.J., Hilton J., Basketter D.A., and Kimber I. Cytokine endpoints for the local lymph node assay: consideration of interferon-gamma and interleukin 12. J. Appl. Toxicol. 19 (1999) 149-155
6. Farrell J., Jenkinson C., Lavergne S.N., Maggs J.L., Kevin Park B., and Naisbitt D.J. Investigation of the immunogenicity of p-phenylenediamine and Bandrowski's base in the mouse. Toxicol. Lett. 185 (2009) 153-159
7. Gerberick G.F., Cruse L.W., and Ryan C.A. Local lymph node assay: differentiating allergic and irritant responses using flow cytometry. Methods 19 (1999) 48-55
8. Gerberick G.F., Ryan C.A., Kern P.S., Schlatter H., Dearman R.J., Kimber I., Patlewicz G.Y., and Basketter D.A. Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods. Dermatitis 16 (2005) 157-202
9. Gerberick G.F., Ryan C.A., Dearman R.J., and Kimber I. Local lymph node assay (LLNA) for detection of sensitization capacity of chemicals. Methods 41 (2007) 54-60
10. ICCVAM, 2009a. Revised Draft Background Review Document-Non-radioactive Murine Local Lymph Node Assay: BrdU-ELISA Test Method Protocol (LLNA: BrdU-ELISA). ICCVAM.
11. ICCVAM, 2009b. Draft ICCVAM Test Method Recommendations-Non-Radioactive Local Lymph Node Assay: BrdU- FC. ICCVAM.
12. Idehara K., Yamagishi G., Yamashita K., and Ito M. Characterization and evaluation of a modified local lymph node assay using ATP content as a non-radio isotopic endpoint. J. Pharmacol. Toxicol. Methods 58 (2008) 1-10
13. Jacobs J.J., Lehe C.L., Hasegawa H., Elliott G.R., and Das P.K. Skin irritants and contact sensitizers induce Langerhans cell migration and maturation at irritant concentration. Exp. Dermatol. 15 (2006) 432-440
14. Ku H.O., Jeong S.H., Kang H.G., Pyo H.M., Cho J.H., Son S.W., Kim H.R., Lee K.J., and Ryu D.Y. Intracellular expression of cytokines and granzyme B in auricular lymph nodes draining skin exposed to irritants and sensitizers. Toxicology 250 (2008) 116-123
15. Porstmann T., Ternynck T., and Avrameas S. Quantitation of 5-bromo-2-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J. Immunol. Methods 82 (1985) 169-179
16. Suda A., Yamashita M., Tabei M., Taguchi K., Vohr H.W., Tsutsui N., Suzuki R., Kikuchi K., Sakaguchi K., Mochizuki K., and Nakamura K. Local lymph node assay with non-radioisotope alternative endpoints. J. Toxicol. Sci. 27 (2002) 205-218
17. Takeyoshi M., Yamasaki K., Yakabe Y., Takatsuki M., and Kimber I. Development of non-radio isotopic endpoint of murine local lymph node assay based on 5-bromo-2′-deoxyuridine (BrdU) incorporation. Toxicol. Lett. 119 (2001) 203-208
18. Takeyoshi M., Sawaki M., Yamasaki K., and Kimber I. Assessment of statistic analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with alpha-hexylcinnamic aldehyde as an example. Toxicology 191 (2003) 259-263
19. Takeyoshi M., Noda S., Yamazaki S., Kakishima H., Yamasaki K., and Kimber I. Assessment of the skin sensitization potency of eugenol and its dimers using a non-radioisotopic modification of the local lymph node assay. J. Appl. Toxicol. 24 (2004) 77-81
20. van den Berg F.A., Baken K.A., Vermeulen J.P., Gremmer E.R., van Steeg H., and van Loveren H. Use of the local lymph node assay in assessment of immune function. Toxicology 211 (2005) 107-114
21. Warbrick E.V., Dearman R.J., Lea L.J., Basketter D.A., and Kimber I. Local lymph node assay responses to paraphenylenediamine: intra- and inter-laboratory evaluations. J. Appl. Toxicol. 19 (1999) 255-260
22. Yamano T., and Shimizu M. Skin sensitization potency and cross-reactivity of p-phenylenediamine and its derivatives evaluated by non-radioactive murine local lymph node assay and guinea-pig maximization test. Contact Dermat. 60 (2009) 193-198