Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children

Journal of Medical Microbiology

Volumn 59, Issue 1, 2010, Pages 25-31

  Contreras, C A ^a   Ochoa, T J ^a,b   Lacher, D W ^c   DebRoy, C ^d   Navarro, A ^e   Talledo, M ^a,f   Donnenberg, M S ^g   Ecker, L ^h   Gil, A I ^h   Lanata, C F ^h,i   Cleary, T G ^b  

^a INSTITUTO DE MEDICINA TROPICAL ALEXANDER VON HUMBOLDT   (Peru)

^b UNIVERSITY OF TEXAS SCHOOL OF PUBLIC HEALTH   (United States)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^d PENNSYLVANIA STATE UNIVERSITY   (United States)

^e UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^f UNIVERSITY OF ANTWERP   (Belgium)

^g UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^h INSTITUTO DE INVESTIGACIÓN NUTRICIONAL   (Peru)

ⁱ UNIVERSIDAD PERUANA DE CIENCIAS APLICADAS   (Peru)

Author keywords

[No Author keywords available]

Indexed keywords

BUNDLIN PILIN PROTEIN OF BUNDLE FORMING PILUS; INTIMIN; PROTEIN DERIVATIVE; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BFPA GENE; BLOOD GROUP TYPING; CHILD; CONTROLLED STUDY; DIARRHEA; DISEASE SEVERITY; EAE GENE; ENTEROPATHOGENIC ESCHERICHIA COLI; GENETIC VARIABILITY; HUMAN; NONHUMAN; PATHOTYPE; PERA GENE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REGULATOR GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ADHESINS, BACTERIAL; CHILD; COHORT STUDIES; DIARRHEA; ENTEROPATHOGENIC ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FIMBRIAE PROTEINS; HUMANS; PERU; REPRESSOR PROTEINS; VIRULENCE;

EID: 73649083666     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.013706-0     Document Type: Article

References (33)

1. Afset, J. E., Bergh, K. & Bevanger, L. (2003). High prevalence of atypical enteropathogenic Escherichia coli (EPEC) in Norwegian children with diarrhoea. J Med Microbiol 52, 1015-1019.
2. Afset, J. E., Bevanger, L., Romundstad, P. & Bergh, K. (2004). Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhoea. J Med Microbiol 53, 1137-1144.
3. Afset, J. E., Bruant, G., Brousseau, R., Harel, J., Anderssen, E., Bevanger, L. & Bergh, K. (2006). Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR. J Clin Microbiol 44, 3703-3711.
4. Bieber, D., Ramer, S. W., Wu, C.-Y., Murray, W. J., Tobe, T., Fernandez, R. & Schoolnik, G. K. (1998). Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli. Science 280, 2114-2118.
5. Blanco, M., Blanco, J. E., Dahbi, G., Alonso, M. P., Mora, A., Coira, M. A., Madrid, C., Juarez, A., Bernardez, M. I. & other authors (2006a). Identification of two new intimin types in atypical enteropathogenic Escherichia coli. Int Microbiol 9, 103-110.
6. Blanco, M., Blanco, J. E., Dahbi, G., Mora, A., Alonso, M. P., Varela, G., Gadea, M. P., Schelotto, F., Gonzalez, E. A. & Blanco, J. (2006b). Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (mB and jR/b2B). J Med Microbiol 55, 1165-1174.
7. Dulguer, M. V., Fabbricotti, S. H., Bando, S. Y., Moreira-Filho, C. A., Fagundes-Neto, U. & Scaletsky, I. C. (2003). Atypical enteropathogenic Escherichia coli strains: phenotypic and genetic profiling reveals a strong association between enteroaggregative E. coli heat-stable enterotoxin and diarrhea. J Infect Dis 188, 1685-1694.
8. Fitzhenry, R. J., Reece, S., Trabulsi, L. R., Heuschkel, R., Murch, S., Thomson, M., Frankel, G. & Phillips, A. D. (2002). Tissue tropism of enteropathogenic Escherichia coli strains belonging to the O55 serogroup. Infect Immun 70, 4362-4368.
9. Fitzhenry, R. J., Stevens, M. P., Jenkins, C., Wallis, T. S., Heuschkel, R., Murch, S., Thomson, M., Frankel, G. & Phillips, A. D. (2003). Human intestinal tissue tropism of intimin epsilon O103 Escherichia coli. FEMS Microbiol Lett 218, 311-316.
10. Garrido, P., Blanco, M., Moreno-Paz, M., Briones, C., Dahbi, G., Blanco, J., Blanco, J. & Parro, V. (2006). STEC-EPEC oligonucleotide microarray: a new tool for typing genetic variants of the LEE pathogenicity island of human and animal shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains. Clin Chem 52, 192-201.
11. Giron, J. A., Ho, A. S. & Schoolnik, G. K. (1991). An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 254, 710-713.
12. Guion, C. E., Ochoa, T. J., Walker, C. M., Barletta, F. & Cleary, T. G. (2008). Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR. J Clin Microbiol 46, 1752-1757.
13. Hedberg, C. W., Savarino, S. J., Besser, J. M., Paulus, C. J., Thelen, V. M., Myers, L. J., Cameron, D. N., Barrett, T. J., Kaper, J. B. & Osterholm, M. T. (1997). An outbreak of foodborne illness caused by Escherichia coli O39:NM, an agent not fitting into the existing scheme for classifying diarrheogenic E. coli. J Infect Dis 176, 1625-1628.
14. Ito, K., Iida, M., Yamazaki, M., Moriya, K., Moroishi, S., Yatsuyanagi, J., Kurazono, T., Hiruta, N. & Ratchtrachenchai, O.-A. (2007). Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains. J Clin Microbiol 45, 1038-1041.
15. Jenkins, C., Lawson, A. J., Cheasty, T., Willshaw, G. A., Wright, P., Dougan, G., Frankel, G. & Smith, H. R. (2003). Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and Eire. Mol Cell Probes 17, 149-156.
16. Jenkins, C., Smith, H. R., Lawson, A. J., Willshaw, G. A., Cheasty, T., Wheeler, J. G. & Tompkins, D. S. (2006). Serotypes, intimin subtypes, and antimicrobial resistance patterns of atypical enteropathogenic Escherichia coli isolated in England from 1993 to 1996. Eur J Clin Microbiol Infect Dis 25, 19-24.
17. Jerse, A. E., Yu, J., Tall, B. D. & Kaper, J. B. (1990). A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci U S A 87, 7839-7843.
18. Knutton, S., Shaw, R., Phillips, A. D., Smith, H. R., Willshaw, G. A., Watson, P. & Price, E. (2001). Phenotypic and genetic analysis of diarrhea-associated Escherichia coli isolated from children in the United Kingdom. J Pediatr Gastroenterol Nutr 33, 32-40.
19. Kozub-Witkowski, E., Krause, G., Frankel, G., Kramer, D., Appel, B. & Beutin, L. (2008). Serotypes and virutypes of enteropathogenic and enterohaemorrhagic Escherichia coli strains from stool samples of children with diarrhoea in Germany. J Appl Microbiol 104, 403-410.
20. Lacher, D. W., Steinsland, H. & Whittam, T. S. (2006). Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. FEMS Microbiol Lett 261, 80-87.
21. Lacher, D. W., Steinsland, H., Blank, T. E., Donnenberg, M. S. & Whittam, T. S. (2007). Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling. J Bacteriol 189, 342-350.
22. Machado, J., Grimont, F. & Grimont, P. A. (2000). Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. Res Microbiol 151, 535-546.
23. Murray, P. R. (2007). Manual of Clinical Microbiology, 9th edn. Washington, DC: American Society for Microbiology.
24. Ochoa, T. J., Barletta, F., Contreras, C. & Mercado, E. (2008). New insights into the epidemiology of enteropathogenic Escherichia coli infection. Trans R Soc Trop Med Hyg 102, 852-856.
25. Ochoa, T. J., Ecker, L., Barletta, F., Mispireta, M., Gil, A., Contreras, C., Molina, M., Amemiya, I., Verastegui, H. & other authors (2009). Age-related susceptibility to infection with diarrheagenic E. coli in infants from peri-urban areas of Lima, Peru. Clin Infect Dis 49, 1694-1702.
26. Orskov, I., Orskov, F., Jann, B. & Jann, K. (1977). Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev 41, 667-710.
27. Ramachandran, V., Brett, K., Hornitzky, M. A., Dowton, M., Bettelheim, K. A., Walker, M. J. & Djordjevic, S. P. (2003). Distribution of intimin subtypes among Escherichia coli isolates from ruminant and human sources. J Clin Microbiol 41, 5022-5032.
28. Ruuska, T. & Vesikari, T. (1990). Rotavirus disease in Finnish children: use of numerical scores for clinical severity of diarrhoeal episodes. Scand J Infect Dis 22, 259-267.
29. Scaletsky, I. C. A., Pedroso, M. Z., Oliva, C. A. G., Carvalho, R. L. B., Morais, M. B. & Fagundes-Neto, U. (1999). A localized adherence-like pattern as a second pattern of adherence of classic enteropathogenic Escherichia coli to HEp-2 cells that is associated with infantile diarrhea. Infect Immun 67, 3410-3415.
30. Scaletsky, I. C., Fabbricotti, S. H., Silva, S. O., Morais, M. B. & Fagundes-Neto, U. (2002). HEp-2-adherent Escherichia coli strains associated with acute infantile diarrhea, Sao Paulo, Brazil. Emerg Infect Dis 8, 855-858.
31. Trabulsi, L. R., Keller, R. & Tardelli Gomes, T. A. (2002). Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 8, 508-513.
32. Vieira, M. A., Andrade, J. R., Trabulsi, L. R., Rosa, A. C., Dias, A. M., Ramos, S. R., Frankel, G. & Gomes, T. A. (2001). Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E. coli (EPEC) serogroups that carry EAE and lack the EPEC adherence factor and Shiga toxin DNA probe sequences. J Infect Dis 183, 762-772.
33. Yatsuyanagi, J., Saito, S., Sato, H., Miyajima, Y., Amano, K.-I. & Enomoto, K. (2002). Characterization of enteropathogenic and enteroaggregative Escherichia coli isolated from diarrheal outbreaks. J Clin Microbiol 40, 294-297.