Atypical Enteropathogenic Escherichia coli Strains: Phenotypic and Genetic Profiling Reveals a Strong Association between Enteroaggregative E. coli Heat-Stable Enterotoxin and Diarrhea

Journal of Infectious Diseases

Volumn 188, Issue 11, 2003, Pages 1685-1694

  Dulguer, Michelle V ^a   Fabbricotti, Sandra H ^a   Bando, Silvia Y ^b   Moreira Filho, Carlos A ^b   Fagundes Neto, Ulysses ^a   Scaletsky, Isabel C A ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI ENTEROTOXIN; INTIMIN; VIRULENCE FACTOR;

ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM ISOLATE; BRAZIL; CONTROLLED STUDY; DIARRHEA; ESCHERICHIA COLI; GENE PROBE; GENE SEQUENCE; GENETIC ANALYSIS; HEP 2 CELL; HUMAN; INFANT; MAJOR CLINICAL STUDY; NONHUMAN; PHENOTYPE; PRESCHOOL CHILD; PRIORITY JOURNAL; RANDOM AMPLIFIED POLYMORPHIC DNA; SEROTYPE; THERMOSTABILITY;

BACTERIAL TOXINS; DIARRHEA; ENTEROTOXINS; ESCHERICHIA COLI; HUMANS; INFANT; INFANT, NEWBORN; PHENOTYPE; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; VIRULENCE;

EID: 0346887081     PISSN: 00221899     EISSN: None     Source Type: Journal    
DOI: 10.1086/379666     Document Type: Article

References (43)

1. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998; 11:142-201.
2. Toledo MRF, Alvariza MCB, Murahovschi J, Ramos SRTS, Trabulsi LR. Enteropathogenic Escherichia coli serotypes and endemic diarrhea in infants. Infect Immun 1983; 39:586-9.
3. Scaletsky ICA, Fabbricotti SH, Silva SO, Morais MB, Fagundes-Neto U. HEp-2-adherent Escherichia coli strains associated with acute infantile diarrhea, São Paulo, Brazil. Emerg Infect Dis 2002; 8:855-8.
4. Scaletsky ICA, Silva MLM, Trabulsi LR. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984; 45:534-6.
5. Scaletsky ICA, Pedroso MZ, Oliva CAG, Carvalho RLB, Morais MB, Fagundes-Neto U. A localized adherence-like pattern as a second pattern of adherence of classic enteropathogenic Escherichia coli to HEp-2 cells that is associated with infantile diarrhea. Infect Immun 1999; 67:3410-5.
6. Baldini MM, Kaper JB, Levine MM, Candy DC, Moon HW. Plasmid-mediated adhesion in enteropathogenic Escherichia coli. J Pediatr Gastroenterol Nutr 1983; 2:534-8.
7. Girón JA, Ho ASY, Schoolnik GK. An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 1991; 254:710-3.
8. Donnenberg MS, Girón JA, Nataro JP, Kaper JB. A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence. Mol Microbiol 1992; 6:3427-37.
9. Gómez-Duarte OG, Kaper JB. A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli. Infect Immun 1995; 63:1767-76.
10. Tobe T, Schoolnik GK, Sohel I, Bustamante VH, Puente JL. Cloning and characterization of bfpTVW, genes required for the transcriptional activation of bfpA in enteropathogenic Escherichia coli. Mol Microbiol 1996; 21:963-75.
11. Moon HW, Whipp SC, Argenzio RA, Levine MM, Gianella RA. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect Immun 1983; 41:1340-51.
12. McDaniel TK, Jarvis KG, Donnenberg MS, Kaper JB. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci USA 1995; 92:1664-8.
13. Jarvis KG, Girón JA, Jerse AE, McDaniel TK, Donnenberg MS, Kaper JB. Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci USA 1995; 92:7996-8000.
14. Jerse AE, Kaper JB. The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun 1991; 59:4302-9.
15. Sperandio V, Kaper JB, Bortolini MR, Neves BC, Keller R, Trabulsi LR. Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes. FEMS Microbiol Lett 1998; 164:133-9.
16. Adu-Bobie J, Frankel G, Bain C, et al. Detection of intimins α, β, γ, and δ, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol 1998; 36:662-8.
17. Frankel G, Phillips AD, Rosenshine I, Dougan G, Kaper JB, Knutton S. Enteropathogenic and enterohemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 1998; 30:911-21.
18. Rosa AC, Mariano AT, Pereira AM, Tibana A, Gomes TAT, Andrade JRC. Enteropathogenicity markers in Escherichia coli isolated from infants with acute diarrhoea and healthy controls in Rio de Janeiro, Brazil. J Med Microbiol 1998; 47:781-90.
19. Vieira AM, Andrade JRC, Trabulsi LR, et al. Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E. coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and Shiga toxin DNA probe sequences. J Infect Dis 2001; 183:762-72.
20. Hedberg CW, Savarino SJ, Besser JM, et al. An outbreak of foodborne illness caused by Escherichia coli O39:NM, an agent not fitting into the existing scheme for classifying diarrheogenic E. coli. J Infect Dis 1997; 176:1625-8.
21. Scaletsky ICA, Fabbricotti SH, Aranda KR, Morais MB, Fagundes-Neto-U. Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli. J Clin Microbiol 2002; 40:1254-8.
22. Ewing WH. Genus Escherichia coli. In: Ewing WH, ed. Edwards and Ewing's identification of Enterobacteriaceae. 4th ed. New York: Elsevier Science, 1986:93-134.
23. Girón JA, Donnenberg MS, Martin WC, Jarvis RG, Kaper JB. Distribution of the bundle-forming pilus structural gene (bfpA) among enteropathogenic Escherichia coli. J Infect Dis 1993; 168:1037-41.
24. Levine MM, Xu J, Kaper JB, et al. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis 1987; 156:175-82.
25. Welch RA, Hell R, Falkow S. Molecular cloning and physical characterization of a chromosomal hemolysin from Escherichia coli. Infect Immun 1983; 42:178-86.
26. Le Bouguénec C, Archambaud M, Labigne A. Rapid and specific detection of the pap, afa, and sfa adhsein-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J Clin Microbiol 1992; 30:1189-93.
27. Savarino SJ, Fox P, Deng Y, Nataro JP. Identification and characterization of a gene cluster mediating enteroaggregative Escherichia coli aggregative adherence fimbria I biogenesis. J Bacteriol 1994; 176:4949-57.
28. Czeczulin JR, Balepur S, Hicks S, et al. Aggregative adherence fimbria II, a second fimbrial antigen mediating aggregative adherence in enteroaggregative Escherichia coli. Infect Immun 1997; 65:4135-45.
29. Bernier C, Gounon P, Le Bouguénec C. Identification of an aggregative adhesion fimbria (AAF) type III-encoding operon in enteroaggregative Escherichia coli as a sensitive probe for detecting the AAF-encoding operon family. Infect Immun 2002; 70:4302-11.
30. Yamamoto T, Echeverria P. Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene sequences in enterotoxigenic E. coli strains pathogenic for humans. Infect Immun 1996; 64:1441-5.
31. Scott DA, Kaper JB. Cloning and sequencing of the genes encoding Escherichia coli cytolethal distending toxin. Infect Immun 1994; 62: 244-51.
32. Oswald E, Pohl P, Jacquemin E, et al. Specific DNA probes to detect Escherichia coli strains producing cytotoxic necrotising factor type 1 or 2. J Med Microbiol 1994; 40:428-34.
33. Yamamoto S, Terai A, Yuri K, Kurazono H, Takeda Y, Yoshida O. Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol Med Microbiol 1995; 12:85-90.
34. Birnboim HC, Doly J. A rapid extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513-23.
35. Mass R. An improved colony hybridization method with significantly increased sensitivity for detection of single genes. Plasmid 1983; 10:296-8.
36. DeVinney R, Puente JL, Gauthier A, Goosney D, Finlay BB. Enterohaemorrhagic and enteropathogenic Escherichia coli use a different Tir-based mechanism for pedestal formation. Mol Microbiol 2001; 41:1445-58.
37. Trabulsi LR, Keller R, Tardelli Gomes TA. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 2002; 8:508-13.
38. Trabulsi LR, Campos LC, Whittam TS, Gomes TAT, Rodrigues J, Gonçalves AG. Traditional and non-traditional enteropathogenic Escherichia coli serogroups. Rev Microbiol 1996; 27:1-6.
39. Scotland SM, Smith HR, Cheasty T, et al. Use of gene probes and adhesion tests to characterise Escherichia coli belonging to enteropathogenic serogroups isolated in the United Kingdom. J Med Microbiol 1996; 44:438-43.
40. Giammanco A, Maggio M, Giammanco G, et al. Characteristics of Escherichia coli strains belonging to enteropathogenic E. coli serogroups isolated in Italy from children with diarrhea. J Clin Microbiol 1996; 34: 689-94.
41. Scaleteky ICA, Silva MLM, Toledo MRF, Davis BR, Blake PA, Trabulsi LR. Correlation between adherence to HeLa cells, serogroups, serotypes, and bioserotypes of Escherichia coli. Infect Immun 1985; 49:528-32.
42. Levine MM, Nataro JP, Karch H, et al. The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. J Infect Dis 1985; 152:550-9.
43. Whittam TS, McGraw EA. Clonal analysis of EPEC serogroups. Rev Microbiol 1996; 27:7-16.