Toxoplasma gondii: Simple duplex RT-PCR assay for detecting SAG1 and BAG1 genes during stage conversion in immunosuppressed mice

Experimental Parasitology

Volumn 124, Issue 2, 2010, Pages 225-231

  Mahittikorn, Aongart ^a   Wickert, Hannes ^b,c   Sukthana, Yaowalark ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

^b Medical Laboratories Dr Staber and Partner   (Germany)

^c UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Duplex RT PCR; Mice; Stage conversion; Toxoplasma gondii

Indexed keywords

BAG1 PROTEIN; PROTOZOAL PROTEIN; SAG1 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRADYZOITE; BRAIN; CEREBELLUM; CONTROLLED STUDY; DIAGNOSTIC VALUE; HEART; IMMUNE DEFICIENCY; LUNG; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE LOCALIZATION; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TACHYZOITE; TOXOPLASMA GONDII; TOXOPLASMOSIS;

ANIMALS; ANTIGENS, PROTOZOAN; BRAIN; FEMALE; HEART; HEAT-SHOCK PROTEINS; IMMUNOCOMPROMISED HOST; IMMUNOSUPPRESSION; LIFE CYCLE STAGES; LUNG; MICE; MICE, INBRED ICR; PROTOZOAN PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS, CEREBRAL;

MUS; TOXOPLASMA GONDII;

EID: 73449121038     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.10.003     Document Type: Article

References (33)

1. Belal U.S., Norose K., Aosai F., Mun H.S., Ahmed A.K., Chen M., Mohamed R.M., Piao L.X., Iwakura Y., and Yano A. Evaluation of the effects of sulfamethoxazole on Toxoplasma gondii loads and stage conversion in IFN-gamma knockout mice using QC-PCR. Microbiology and Immunology 48 (2004) 185-193
2. Chabbert E., Lachaud L., Crobu L., and Bastien P. Comparison of two widely used PCR primer systems for detection of Toxoplasma in amniotic fluid, blood, and tissues. Journal of Clinical Microbiology 42 (2004) 1719-1722
3. Charles E., Callegan M.C., and Blader I.J. The SAG1 Toxoplasma gondii surface protein is not required for acute ocular toxoplasmosis in mice. Infection and Immunity 75 (2007) 2079-2083
4. Contini C., Cultrera R., Seraceni S., Segala D., Romani R., Fainardi E., Cinque P., Lazzarin A., and Delia S. The role of stage-specific oligonucleotide primers in providing effective laboratory support for the molecular diagnosis of reactivated Toxoplasma gondii encephalitis in patients with AIDS. Journal of Medical Microbiology 51 (2002) 879-890
5. Contini C., Giuliodori M., Cultrera R., and Seraceni S. Detection of clinical-stage specific molecular Toxoplasma gondii gene patterns in patients with toxoplasmic lymphadenitis. Journal of Medical Microbiology 55 (2006) 771-774
6. Cultrera R., Seraceni S., Segala D., and Contini C. Expression of toxoplasmic 65 kDa cystic mRNA by RT-PCR in patients with Toxoplasma gondii infection relapses. The Journal of Eukaryotic Microbiology Suppl. (2001) 193S-194S
7. Cultrera R., Seraceni S., and Contini C. Efficacy of a novel reverse transcriptase-polymerase chain reaction (RT-PCR) for detecting Toxoplasma gondii bradyzoite gene expression in human clinical specimens. Molecular and Cellular Probes 16 (2002) 31-39
8. Dubey J.P., Lindsay D.S., and Speer C.A. Structures of Toxoplasma gondii tachyzoites, bradyzoites, and sporozoites and biology and development of tissue cysts. Clinical Microbiology Reviews 11 (1998) 267-299
9. Ferguson D.J., and Parmley S.F. Toxoplasma gondii MAG1 protein expression. Trends in Parasitology 18 (2002) 482
10. Ferguson D.J. Use of molecular and ultrastructural markers to evaluate stage conversion of Toxoplasma gondii in both the intermediate and definitive host. International Journal for Parasitology 34 (2004) 347-360
11. Gazzinelli R., Xu Y., Hieny S., Cheever A., and Sher A. Simultaneous depletion of CD4+ and CD8+ T lymphocytes is required to reactivate chronic infection with Toxoplasma gondii. Journal of Immunology 149 (1992) 175-180
12. Gross U., Holpert M., and Goebel S. Impact of stage differentiation on diagnosis of toxoplasmosis. Annali Dell'Istituto Superiore di Sanità 40 (2004) 65-70
13. Hill D., and Dubey J.P. Toxoplasma gondii: transmission, diagnosis and prevention. Clinical Microbiology and Infection 8 (2002) 634-640
14. Jalal S., Nord C.E., Lappalainen M., and Evengard B. Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR. Clinical Microbiology and Infection 10 (2004) 937-939
15. Joseph P., Calderon M.M., Gilman R.H., Quispe M.L., Cok J., Ticona E., Chavez V., Jimenez J.A., Chang M.C., Lopez M.J., and Evans C.A. Optimization and evaluation of a PCR assay for detecting toxoplasmic encephalitis in patients with AIDS. Journal of Clinical Microbiology 40 (2002) 4409-4503
16. Kang K.N., Choi I.U., Shin D.W., and Lee Y.H. Cytokine and antibody responses of reactivated murine toxoplasmosis upon administration of dexamethasone. The Korean Journal of Parasitology 44 (2006) 209-219
17. Lekutis C., Ferguson D.J., Grigg M.E., Camps M., and Boothroyd J.C. Surface antigens of Toxoplasma gondii: variations on a theme. International Journal for Parasitology 31 (2001) 1285-1292
18. Luft B.J., and Remington J.S. Toxoplasmic encephalitis in AIDS. Clinical Infectious Diseases 15 (1992) 211-222
19. Lyons R.E., Lyons K., McLeod R., and Roberts C.W. Construction and validation of a polycompetitor construct (SWITCH) for use in competitive RT-PCR to assess tachyzoite-bradyzoite interconversion in Toxoplasma gondii. Parasitology 123 (2001) 433-439
20. Lyons R.E., McLeod R., and Roberts C.W. Toxoplasma gondii tachyzoite-bradyzoite interconversion. Trends in Parasitology 18 (2002) 198-201
21. Mariuz P., and Steigbigel R.T. Toxoplasma infection in HIV-infected patients. In: Joynson D.H.M., and Wreghitt T.G. (Eds). Toxoplasmosis: A Comprehensive Clinical Guide (2001), Cambridge University Press, Cambridge, UK 147-177
22. Nicoll S., Wright S., Maley S.W., Burns S., and Buxton D. A mouse model of recrudescence of Toxoplasma gondii infection. Journal of Medical Microbiology 46 (1997) 263-266
23. Odaert H., Soête M., Fortier B., Camus D., and Dubremetz J.F. Stage conversion of Toxoplasma gondii in mouse brain during infection and immunodepression. Parasitology Research 82 (1996) 28-31
24. Odberg-Ferragut C., Soete M., Engels A., Samyn B., Loyens A., Van Beeumen J., Camus D., and Dubremetz J.F. Molecular cloning of the Toxoplasma gondii sag4 gene encoding an 18 kDa bradyzoite specific surface protein. Molecular and Biochemicak Parasitology 82 (1996) 237-244
25. OIE, 2008. Toxoplasmosis. In: OIE Manual of Diagnostic tests and Vaccines for Terrestrial Animals, vol. 2. p. 1286.
26. Parmley S.F., Yang S., Harth G., Sibley L.D., Sucharczuk A., and Remington J.S. Molecular characterization of a 65-kilodalton Toxoplasma gondii antigen expressed abundantly in the matrix of tissue cysts. Molecular and Biochemical Parasitology 66 (1994) 283-296
27. Rachlin J., Ding C.M., Cantor C., and Kasif S. muPlex: multi-objective multiplex PCR assay design. Nucleic Acids Research 33 Web Server Issue (2005) W544-W547
28. Sukthana Y. Toxoplasmosis: beyond animals to humans. Trends in Parasitology 22 (2006) 137-142
29. Switaj K., Master A., Skrzypczak M., and Zaborowski P. Recent trends in molecular diagnostics for Toxoplasma gondii infections. Clinical Microbiology and Infection 11 (2005) 170-176
30. Ueno A., Dautu G., Munyaka B., Carmen G., Kobayashi Y., and Igarashi M. Toxoplasma gondii: identification and characterization of bradyzoite-specific deoxyribose phosphate aldolase-like gene (TgDPA). Experimental Parasitology 121 (2009) 55-63
31. Unno A., Suzuki K., Batanova T., Cha S.Y., Jang H.K., Kitoh K., and Takashima Y. Visualization of Toxoplasma gondii stage conversion by expression of stage-specific dual fluorescent proteins. Parasitology 136 (2009) 579-588
32. Waree P., Ferguson D.J., Pongponratn E., Chaisri U., and Sukthana Y. Immunohistochemical study of acute and chronic toxoplasmosis in experimentally infected mice. The Southeast Asian Journal of Tropical Medicine and Public Health 38 (2007) 223-231
33. Weiss L.M., and Kim K. The development and biology of bradyzoites of Toxoplasma gondii. Frontiers in Bioscience 1 (2000) D391-D405