Construction and validation of a polycompetitor construct (SWITH) for use in competitive RT-PCR to assess tachyzoite-bradyzoite interconversion in Toxoplasma gondii

Parasitology

Volumn 123, Issue 5, 2001, Pages 433-439

  Lyons, R E ^a,c   Lyons, K ^a,d   Mcleod, R ^b   Roberts, C W ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^b UNIVERSITY OF CHICAGO   (United States)

^c QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

^d UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Bradyzoites; Conversion; CRT PCR; SAG2; Tachyzoites; Toxoplasma gondii

Indexed keywords

GENE PRODUCT; PROTEIN LDH2; PROTEIN SAG2A; UNCLASSIFIED DRUG;

ACUTE DISEASE; ARTICLE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE CONSTRUCT; GENE EXPRESSION; HOUSEKEEPING GENE; LIFE CYCLE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PLASMID; PRIORITY JOURNAL; RECURRENT DISEASE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TOXOPLASMA GONDII; VALIDATION PROCESS;

ANIMALS; ANTIGENS, PROTOZOAN; ANTIGENS, SURFACE; BASE SEQUENCE; DNA, PROTOZOAN; GENE EXPRESSION REGULATION; GENES, SWITCH; ISOENZYMES; L-LACTATE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PROTOZOAN; TOXOPLASMA; TOXOPLASMOSIS; TRANSCRIPTION, GENETIC; TUBULIN;

PROTOZOA; TOXOPLASMA GONDII;

EID: 0034755403     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: 10.1017/S003118200100868X     Document Type: Article

References (21)

1. Cloning and characterization of a bradyzoite-specifically expressed gene (hsp30/bag1) of Toxoplasma gondii, related to genes encoding small heat-shock proteins of plants
2. Standardization of mRNA titration using a polymerase chain reaction method involving co-amplification with a multispecific internal control
3. Diagnosis and treatment of congenital toxoplasmosis
4. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
5. Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA
6. Advances in the life cycle of Toxoplasma gondii
7. Competitive PCR for quantitation of mRNA
8. Acute cerebral toxoplasmosis is induced by in vivo neutralization of TNF-alpha and correlates with the down-regulated expression of inducible nitric oxide synthase and other markers of macrophage activation
9. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
10. Purification and characterization of Toxoplasma gondii tachyzoite DNA
11. Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies
12. Toxoplasmic encephalitis in AIDS
13. Toxoplasma gondii: Identification of a developmentally regulated family of genes related to SAG2
14. The alpha- and beta-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns
15. Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii
16. Constructing polycompetitor cDNAs for quantitative PCR
17. Pathogenesis of toxoplasmic retinochoroiditis
18. Inhibition of nitric oxide production exacerbates chronic ocular toxoplasmosis
19. Toxoplasma gondii: Kinetics of bradyzoitetachyzoite interconversion in vitro
20. Toxoplasma gondii: From animals to humans
21. Toxoplasma gondii expresses two distinct lactate dehydrogenase homologous genes during its life cycle in intermediate hosts