Rapid pathogen detection using a microchip PCR array instrument

Clinical Chemistry

Volumn 44, Issue 10, 1998, Pages 2191-2194

  Belgrader, Phillip ^a   Benett, William ^a   Hadley, Dean ^a   Long, Gary ^b   Mariella Jr , Raymond ^a   Milanovich, Fred ^a   Nasarabadi, Shanavaz ^a   Nelson, William ^b   Richards, James ^a   Stratton, Paul ^a  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^b NAVAL MEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACILLUS SUBTILIS; DATA ANALYSIS; DEMOGRAPHY; ENVIRONMENTAL FACTOR; ERWINIA; IMMUNOASSAY; NUCLEIC ACID ANALYSIS; PATHOGENESIS; POLYMERASE CHAIN REACTION; VIRUS INFECTION; WESTERN EQUINE ENCEPHALOMYELITIS ALPHAVIRUS;

ALPHAVIRUS; BACILLUS ANTHRACIS; BACILLUS SUBTILIS; EQUIDAE; ERWINIA; PANTOEA AGGLOMERANS; WESTERN EQUINE ENCEPHALOMYELITIS VIRUS; YERSINIA PESTIS;

EID: 7344231632     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/44.10.2191     Document Type: Article

References (19)

1. Satcher DS. Emerging infections: getting ahead of the curve. Emerg Infect Dis 1995;1:1-6.
2. Morse SS. Factors in the emergence of infectious disease. Emerg Infect Dis 1995;1:7-15.
3. Wilson ME. Travel and the emergence of infectious diseases. Emerg Infect Dis 1995;1:39-46.
4. Simon JD. Biological terrorism: preparing to meet the threat. JAMA 1997;278:428-30.
5. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA-polymerase. Science 1988;239:487-91.
6. Tang YW, Procop GW, Pershing DH. Molecular diagnostics of infectious diseases. Clin Chem 1997;43:2021-38.
7. Holland PM, Abramson RD, Watson R, Will S, Saiki RK, Gelfand DH. Detection of specific polymerase chain-reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA-polymerase. Clin Chem 1992;38:462-3.
8. Livak KJ, Flood SJA, Marmaro J, Guisti W, Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic-acid hybridization. PCR Methods Appl 1995;4:337-62.
9. Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotech 1997;14:303-8.
10. Nazarenko IA, Bhatnagar SK, Hohman RJ. A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res 1998;25:2516-21.
11. Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA, Balis UJ. The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 1997;22:176-81.
12. Haedicke W, Wolf H, Ehret W, Reischl U. Specific and sensitive 2-step polymerase chain-reaction assay for the detection of Salmonella species. Eur J Clin Microbiol Infect Dis 1996;15: 603-7.
13. Loeffelhotz MJ, Lewinski CA, Silver SR, Purohit AP, Herman SA, Buonagurio DA, Dragon EA. Detection of Chlamydia trachomatis endocervical specimens by polymerase chain-reaction. J Clin Microbiol 1992;30:2847-51.
14. Northrup MA, Benett B, Hadley D, Landre P, Lehew S, Richards J, Stratton P. A miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers. Anal Chem 1998;70:918-22.
15. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997;22:130-1.
16. Ibrahim MS, Lofts RS, Jahrling PB, Henchal EA, Weedn VW, Northrup MA, Belgrader P. Real-time microchip PCR for detecting single base differences in viral and human DNA. Anal Chem 1998;70:2013-7.
17. Belgrader P, Smith JK, Weedn VW, Northrup MA. Rapid PCR identity testing using a battery-powered miniature thermal cycler. J Forensic Sci 1998;43:315-9.
18. Wooley AT, Hadley D, Landre P, deMello AJ, Mathies RA, Northrup MA. Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. Anal Chem 1996;68:4081-6.
19. Cheng J, Sheldon EL, Wu L, Uribe A, Gerrue LO, Carrino J, et al. Preparation and hybridization analysis of DNA/RNA from E. coli on microfabricated bioelectronic chips. Nat Biotech 1998;16:541-6.