Irradiation induces G2/M cell cycle arrest and apoptosis in p53-deficient lymphoblastic leukemia cells without affecting Bcl-2 and Bax expression

Cell Death and Differentiation

Volumn 5, Issue 8, 1998, Pages 687-693

  Strasser Wozak, Elisabeth M C ^a   Hartmann, Bernd L ^a   Geley, Stephan ^a   Sgonc, Roswitha ^a   Böck, Günther ^a   Oliveira Dos Santos, Antonio J ^a   Hattmannstorfer, Rosa ^a   Wolf, Hugo ^a   Pavelka, Margit ^a   Kofler, Reinhard ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

Author keywords

Apoptosis; Bax; bcl 2; CCRF CEM; Cell cycle; Gamma irradiation; Human leukemia; p21 WAF1; p53

Indexed keywords

PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53;

ACUTE LYMPHOBLASTIC LEUKEMIA; APOPTOSIS; ARTICLE; CELL CYCLE G2 PHASE; ELECTRON MICROSCOPY; GAMMA IRRADIATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; TUMOR CELL LINE; TUMOR SUPPRESSOR GENE;

EID: 7344222588     PISSN: 13509047     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cdd.4400402     Document Type: Article

References (39)

1. 2/M when it is transactivation defective. EMBO J. 14: 4994-5005
2. Bracey TS, Miller JC, Preece A and Paraskeva C (1995) gamma-radiation-induced apoptosis in human colorectal adenoma and carcinoma cell lines can occur in the absence of wild type p53. Oncogene 10:2391-2396
3. Canman CE, Chen C-Y, Lee M-H and Kastan MB (1994) DNA damage responses: p53induction, cell cycle perturbations, and apoptosis. Cold Spring Harbor Symp. Quant. Biol. 59:277-286
4. Chen M, Quintans J, Fuks Z, Thompson C, Kufe DW and Weichseibaum RR (1995) Suppression of Bcl-2 messenger RNA production may mediate apoptosis aftsr ionizing radiation, tumor necrosis factor α, and ceramide Cancer Res. 55: 991-994
5. Cheng J and Haas M (1990) Frequent mutations in the p53 tumor suppressor gene in human leukemia T-cell lines. Mol. Cell. Biol 10:5502-5509
6. Clarke AR, Purdie CA, Harrison DJ, Morris RG, Bird CC, Hooper ML and Wyllie AH (1993) Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature 362 849-852
7. Cory S (1995) Regulation of lymphocyte survival by the BCL-2 gene family Annu Rev. Immunol. 13:513-543
8. Danova M, Giordano M, Mazzini G and Riccardi A (1990) Expression of p53 protein during cell cycle measured by flow cytometry in human leukemia. Leuk. Res. 14. 417-422
9. 1 arrest and apoptosis Proc. Natl. Acad. Sci. USA 92:0019-9023
10. Dulic V, Kaufmann WK, Wilson SJ, Tlsty TD, Lees E, Harper JW, Elledge SJ and Reed SI (1994) p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced 1 arrest. Cell 76, 1013-1023
11. El-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW and Vogelstein B (1993) WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-825
12. Geley S, Hartmann BL, Hala M, Strasser-Wozak EMC, Kapelari K and Kofler R (1996) Resistance to glucocorticoid-mduced apoptosis in human T-cell acute lymphoblastic leukemia CEM-C1 cells is due to insufficient glucocorticoid receptor expression. Cancer Res. 56:5033-5038
13. Geley S, Hartmann BL, Hattmannstorfer R, Löffler M, Ausser-Iechner MJ, Bernhard D, Sgonc R, Strasser-Wozak EMC, Ebner M, Auer B and Kofler R (1997a) P53-induced apoptosis in the human T-ALL cell line CCRF-CEM Oncogene 15, 2429-2437
14. Geley S, Hartmann BL, Kapelari K, Egle A, Villunger A, Heidacher D, Greil R, Auer B and Kofler R (1997b) The interleukin 1β-converting enzyme inhibitor crmA prevents Apo1/fas- but not glucocorticoidinduced poly(ADP-nbose) polymerase cleavage and apoptosis in lymphoblastic leukemia cells. FEBS Lett, 402, 36-40
15. Imamura J, Miyoshi I and Koeffler HP (1994) p53 in hematologic malignancies. Blood 84, 2412-2421
16. 2 checkpoint in p53-independent apoptosis induction by X-irradiation. Mol. Cell. Biol. 15: 5849-5857
17. Kluck RM, Bossy-Wetzel E, Green DR and Newmeyer DD (1997) The release of cytochrome c from mitochondria. A primary site for Bcl-2 regulation of apoptosis. Science 275 1132-1136
18. Komarova EA, Chernov MV, Franks R, Wang KH, Armin G, Zelnick CR, Chin DM, Bacus SS, Stark GR and Gudkov AV (1997) Transgenic mice with p53-responsive lacZ: P53 activity vanes dramatically during development and determines radiation and drug sensitivity in. EMBO J. EMBO J. 16: 1391-1400
19. Kroemer G (1997) The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nature Med 3 614-620
20. Lane DP (1992) Cancer: p53, guardian of the genome. Nature 358, 15-16
21. Lowe SW, Schmitt EM, Smith SW, Osborne BA and Jacks T(1993) p53 is required for radiation-induced apoptosis in mouse thymocytes Nature 362: 847-849
22. MacFarlane M, Jones NA, Dive C and Cohen GM (1996) DNA-Damaging agents induce both p53-dependent and p53-independent apoptosis in immature thymocytes. Mol. Pharmacol. 50:900-911
23. Martin SJ, Reutelingsperger CPM and Green DR (1996) Annexin V: a specific probe for apoptotic cells. In Techniques in apoptosis, In: CotterTG and Martin SJ, (eds) London: Portland Press Ltd pp. 107-120
24. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F and Riccardi C (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry J. Immunol. Methods 139:271-279
25. Norman MR and Thompson EB (1977) Characterization of a glucocorticoid-sensitive human lymphoid cell line Cancer Res. 37: 3785-3791
26. Oltvai ZN and Korsmeyer SJ (1994) Checkpoints of dueling dimers foil death wishes. Cell 79: 189-192
27. Pellegata NS, Antoniono RJ, Redpath JL and Stanbridge EJ (1996) DNA damage and p53-mediated cell cycle arrest: A reevaluation. Proc. Natl. Acad. Sci. USA 93: 15209-15214
28. Prokocimer M and Rotter V (1994) Structure and function of p53 in normal cells and their aberrations in cancer cells. Projection on the hematologic cell lineages. Blood 84:2391-2411
29. Reed JC, Miyashita T, Takayama S, Wang HG, Sato T, Krajewski S, Aimé-Sempé C, Bodrug S, Kitada S and Hanada M (1996) BCL-2 family proteins: Regulators of cell death involved in the pathogenesis of cancer and resistance to therapy. J. Cell. Biochem. 60:23-32
30. Sentman CL, Shutter JR, Hockenbery D, Kanagawa O and Korsmeyer SJ (1991) Bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes. Cell 67: 879-888
31. Smith ML, Chen I-T, Zhan Q, Bae I, Chen C-Y, Gilmer TM, Kastan MB, O'Connor PM and Fomace AJ, r (1994) Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen Science 266: 1376-1380
32. Strasser A, Harris AW and Cory S (1991) bcl-2 Transgene inhibits T cell death and perturbs thymic self-censorship. Cell 67:889-899
33. Strasser A, Harris AW, Jacks T and Cory S (1994) DNA damage can induce apoptosis in proliferating lymphoid cells via p53-independent mechanisms inhibitable by Bcl-2. Cell 79: 329-339
34. Strasser-Wozak EMC, Hattmannstorfer R, Hála M, Hartmann BL, Regt M, Geley S and Kofler R (1995) Splice site mutation in the glucocorticoid receptor gene causes resistance to glucocorticoid-induced apoptosis in a human acute leukemic cell line. Cancer Res. 55:348-353
35. Vogelstein B and Kinzler KW (1992) p53 Function and dysfunction. Cell 70: 523-526
36. White E, Chiou S-K, Rao L, Sabbatini P and Lin H-J (1994) Control of p53-dependent apoptosis by E1B, Bcl-2, and Ha-ras proteins. Cold Spring Harbor Symp. Quant. Biol. 59: 395-402
37. White E (1995) Regulation of p53-dependent apoptosis by E1A and E1B. Curr. Top. Microbiol. Immunol 199:33-58
38. Yin X-M, Oltvai ZN, Veis-Novack DJ, Linette GP and Korsmeyer SJ (1994) Bcl-2gene family and the regulation of programmed cell death. Cold Spring Harbor Symp. Quant. Biol. 59: 387-394
39. Yonish-Rouach E, Resnitzky D, Lotem J, Sachs L, Kimchi A and Oren M (1991) Wildtype p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6. Nature 352: 345-347