Around-the-objective total internal reflection fluorescence microscopy

Applied Optics

Volumn 48, Issue 32, 2009, Pages 6120-6131

  Burghardt, Thomas P ^a   Hipp, Andrew D ^a   Ajtai, Katalin ^a  

^a MAYO GRADUATE SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FIELD EMISSION; FLUORESCENCE; FLUORESCENCE MICROSCOPY; FLUOROPHORES; GLASS; LASER BEAMS; LASER EXCITATION; LIGHT; REFRACTIVE INDEX;

BACKGROUND LIGHT; EPI-ILLUMINATION; HIGH NUMERICAL APERTURE OBJECTIVE; MICROSCOPE OBJECTIVE; MICROSCOPE OPTICS; MICROSCOPIC OPTICS; NEAR-FIELD EMISSION; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

EVANESCENT FIELDS;

ANIMAL; ARTICLE; CELL CULTURE; CYTOLOGY; EVALUATION; FLUORESCENCE MICROSCOPY; HEART MUSCLE CELL; IMAGE ENHANCEMENT; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SWINE;

ANIMALS; CELLS, CULTURED; IMAGE ENHANCEMENT; MICROSCOPY, FLUORESCENCE; MYOCYTES, CARDIAC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SWINE;

EID: 73349099991     PISSN: 1559128X     EISSN: 15394522     Source Type: Journal    
DOI: 10.1364/AO.48.006120     Document Type: Article

References (26)

1. N. C. Shaner, P. A. Steinbach, and R. Y. Tsien, "A guide to choosing fluorescent proteins, " Nature Methods 2, 905-909 (2005).
2. R. E. Thompson, D. R. Larson, andW.W.Webb, "Precise nanometer localization analysis for individual fluorescent probes, " Biophys. J. 82, 2775-2783 (2002).
3. N. Bobroff, "Position measurement with a resolution and noise-limited instrument, " Rev. Sci. Instrum. 57, 1152-1157 (1986).
4. A. Yildiz, J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin, "Myosin V walks hand-over-hand: single fluorophore imaging with 1:5nm localization, " Science 300, 2061-2065 (2003).
5. T. Ruckstuhl and S. Seeger, "Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy, " Opt. Lett. 29, 569-571 (2004).
6. T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, "In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy, " Biophys. J. 90, 4662-4671 (2006).
7. J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, "Application of surface plasmon coupled emission to study of muscle, " Biophys. J. 91, 2626-2635 (2006).
8. T. Ruckstuhl and D. Verdes, "Supercritical angle fluorescence (SAF) microscopy, " Opt. Express 12, 4246-4254 (2004).
9. M. G. L. Gustafsson, "Nonlinear structured-illumination microscopy: Wide field fluorescence imaging with theoretically unlimited resolution, " Proc. Natl. Acad. Sci. USA 102, 13081-13086 (2005).
10. G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Luhrmann, R. Jan, C. Eggeling, and S. W. Hell, "Macromolecular-scale resolution in biological fluorescence microscopy, " Proc. Natl. Acad. Sci. USA 103, 11440-11445 (2006).
11. M. A. Lieb, J. M. Zavislan, and L. Novotny, "Single-molecule orientations determined by direct emission pattern imaging, " J. Opt. Soc. Am. B 21, 1210-1215 (2004).
12. T. P. Burghardt and K. Ajtai, "Mapping microscope object polarized emission to the back focal plane pattern, " J. Biomed. Opt. 14, 034036 (2009).
13. D. Axelrod, "Total internal reflection fluorescence microscopy in cell biology, " Meth. Enzymol. 361, 1-33 (2003).
14. D. Axelrod and G. M. Omann, "Combinatorial microscopy, " Nat. Rev. Mol. Cell Biol. 7, 944-952 (2006).
15. A. L. Stout and D. Axelrod, "Evanescent field excitation of fluorescence by epi-illumination microscopy, " Appl. Opt. 28, 5237-5242 (1989).
16. D. Axelrod, "Cell-substrate contacts illuminated by total internal reflection fluorescence, " J. Cell Biol. 89, 141-145 (1981).
17. T. P. Burghardt, K. Ajtai, D. K. Chan, M. F. Halstead, J. Li, and Y. Zheng, "GFP tagged regulatory light chain monitors single myosin lever-arm orientation in a muscle fiber, " Biophys. J. 93, 2226-2239 (2007).
18. T. P. Burghardt, K. Ajtai, and J. Borejdo, "In situ single molecule imaging with attoliter detection using objective total internal reflection confocal microscopy, " Biochemistry 45, 4058-4068 (2006).
19. A. Yoshida and T. Asakura, "Electromagnetic field near the focus of gaussian beams, " Optik (Jena) 41, 281-292 (1974).
20. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems. II. Structure of the image field in an aplanatic system, " Proc. R. Soc. A 253, 358-379 (1959).
21. T. P. Burghardt and N. L. Thompson, "Evanescent intensity of a focused Gaussian light beam undergoing total internal reflection in a prism, " Opt. Eng. 23, 62-67 (1984).
22. E. H. Hellen and D. Axelrod, "Fluorescence emission at dielectric and metal-film interfaces, " J. Opt. Soc. Am. B 4, 337-350 (1987).
23. D. Axelrod, "Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization, " Biophys. J. 26, 557-573 (1979).
24. T. P. Burghardt and D. Axelrod, "Total internal reflection/ fluorescence photobleaching recovery study of serum albumin adsorption dynamics, " Biophys. J. 33, 455-467 (1981).
25. A. Mattheyses and D. Axelrod, "Fluorescence emission patterns near glass and metal-coated surfaces investigated with back focal plane imaging, " J. Biomed. Opt. 10, 054007 (2005).
26. D. Axelrod, T. P. Burghardt, and N. L. Thompson, "Total internal reflection fluorescence, " Annu Rev Biophys Bioeng 13, 247-268 (1984).