A novel direct screening method for alkyl glucoside production by glucosidases expressed in E. coli in 96-well plates

Journal of Biotechnology

Volumn 145, Issue 2, 2010, Pages 186-192

  Gräber, Martin ^a   Andersson, Mats ^b   Rundbäck, Fabian ^a   Pozzo, Tania ^a   Karlsson, Eva Nordberg ^a   Adlercreutz, Patrick ^a  

^a LUND UNIVERSITY   (Sweden)

^b Alligator Bioscience AB   (Sweden)

Author keywords

Glucosidase; Bgl3B; Glycoside hydrolase; Reverse hydrolysis

Indexed keywords

96-WELL FORMAT; 96-WELL PLATE; ALKYL GLUCOSIDES; ALKYLGLYCOSIDES; AQUEOUS PHASE; E. COLI; ENZYMATIC SYNTHESIS; GLUCOPYRANOSIDE; GLUCOSIDASE; GLYCOSIDE HYDROLASE FAMILY 1; GLYCOSIDE HYDROLASES; HEXANOL; HIGH PURITY; LOW TOXICITY; ORGANIC PHASE; REVERSE HYDROLYSIS; SCREENING ASSAYS; SCREENING METHODS; SULPHONIC ACIDS; TWO PHASE SYSTEMS; WELL PLATES;

ALCOHOLS; ALKYLATION; ASSAYS; BIODEGRADATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GLUCOSE; GLUCOSE OXIDASE; HYDROLYSIS; ORGANIC ACIDS; PETROLEUM RESERVOIR EVALUATION; SURFACE ACTIVE AGENTS; SYNTHESIS (CHEMICAL);

PHASE SEPARATION;

2,2' AZINOBIS(3 ETHYLBENZOTHIAZOLINE 6 SULFONIC ACID); 4 NITROPHENYL BETA DEXTRO GLUCOPYRANOSIDE; ALKYL GLUCOSIDE; BETA GLUCOSIDASE; GLUCOPYRANOSIDE; GLUCOSE OXIDASE; GLUCOSIDASE; GLUCOSIDE; HEXYL GLUCOSIDE; PEROXIDASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BIOTECHNOLOGICAL PRODUCTION; CONTROLLED STUDY; CYTOLYSIS; ENZYME ASSAY; ENZYME DEGRADATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE HYDROLYSIS; SCREENING; SPECTROPHOTOMETRY; TOXICITY;

BIOLOGICAL ASSAY; CELL CULTURE TECHNIQUES; ENZYME ACTIVATION; ESCHERICHIA COLI; GLUCOSIDASES; GLUCOSIDES; PROTEIN ENGINEERING;

EID: 72949112011     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.11.005     Document Type: Article

References (20)

1. Aharoni A., Griffiths A.D., and Tawfik D.S. High-throughput screens and selections of enzyme-encoding genes. Curr. Opin. Chem. Biol. 9 (2005) 210-216
2. Arnold F.H. Design by directed evolution. Acc. Chem. Res. 31 (1998) 125-131
3. Crout D.H.G., and Vic G. Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis. Curr. Opin. Chem. Biol. 2 (1998) 98-111
4. Ducret A., Trani M., and Lortie R. Screening of various glycosidases for the synthesis of octyl glucoside. Biotechnol. Bioeng. 77 (2002) 752-757
5. Grabski A., Mehler M., and Drott D. The overnight express autoinduction system: high-density cell growth and protein expression while you sleep. Nat. Methods 2 (2005) 233-235
6. Hibbert E.G., and Dalby P.A. Directed evolution strategies for improved enzymatic performance. Microb. Cell Fact. 4 (2005) 29
7. Koskinen A.M.P., and Klibanov A.M. Enzymatic Reactions in Organic Media (1996), Chapman & Hall, London
8. Konarzycka-Bessler M., and Bornscheuer U.T. A high-throughput-screening method for determining the synthetic activity of hydrolases. Angew. Chem., Int. Ed. 42 (2003) 1418-1420
9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
10. Ljunger G., Adlercreutz P., and Mattiasson B. Enzymatic synthesis of octyl-β-glucoside in octanol at controlled water activity. Enzyme Microb. Technol. 16 (1994) 751-755
11. Panitrarux C., Adachi S., Araki Y., and Matsuno R. Equilibrium yield of n-alkyl-β-d-glucoside through condensation of glucose and n-alcohol by β-glucosidase in a biphasic system. Enzyme Microb. Technol. 17 (1995) 21-40
12. Raabo E., and Terkildsen T.C. On the enzymatic determination of blood glucose. Scand. J. Clin. Lab. Invest. 12 (1960) 402-407
13. Sellek G.A., and Chaudhuri J.B. Biocatalysis in organic media using enzymes from extremophiles. Enzyme Microb. Technol. 25 (1999) 471-482
14. Schmidt R.R. Recent developments in the synthesis of glycoconjugates. Pure Appl. Chem. 61 (1989) 1257-1270
15. Schmitke J.L., Wescott C.R., and Klibanov A.M. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents. J. Am. Chem. Soc. 118 (1996) 3360-3365
16. Turner C., Turner P., Jacobson G., Waldebäck M., Sjöberg P., Nordberg Karlsson E., and Markides K. Subcritical water extraction and beta-glucosidase-catalyzed hydrolysis of quercetin in onion waste. Green Chem. 8 (2006) 949-959
17. Turner P., Mamo G., and Nordberg Karlsson E. Potential and utilization of thermophiles and thermostable enzymes in biorefining. Microb. Cell Factories 6 (2007) 9
18. Turner P., Svensson D., Adlercreutz P., and Nordberg Karlsson E. A novel variant of Thermotoga neapolitana B-glucosidase B is an efficient catalyst for the synthesis of alkyl glucosides by transglycosylation. J. Biotechnol. 130 (2007) 67-74
19. van Rantwijk F., Woudenberg-van Oosterom M., and Sheldon R.A. Glycosidase-catalysed synthesis of alkyl glycosides. J. Mol. Catal. B: Enzym. 6 (1999) 511-532
20. Vulfson E.N., Patel R., Beecher J.E., Andrews A.T., and Law B.A. Glycosidases in organic solvents. I. Alkyl-β-glucoside synthesis in a water-organic two-phase system. Enzyme Microb. Technol. 12 (1990) 950-954