Construction of synthetic open reading frame encoding human interferon alpha 2b for high expression in Escherichia coli and characterization of its gene product

Journal of Biotechnology

Volumn 145, Issue 2, 2010, Pages 193-198

  Retnoningrum, Debbie S ^a   Ningrum, Ratih A ^a   Kurniawan, Yohanes N ^a   Indrayati, Ana ^a   Rachmawati, Heni ^a  

^a BANDUNG INSTITUTE OF TECHNOLOGY   (Indonesia)

Author keywords

Codon optimization; Escherichia coli; High expression; Human interferon 2b; Inclusion bodies; Synthetic ORF

Indexed keywords

BASE PAIRS; CODON OPTIMIZATION; DNA BANDS; E. COLI; ERROR RATE; EXPRESSION IN ESCHERICHIA COLI; EXPRESSION LEVELS; FUSION PROTEINS; GENE ASSEMBLY; GENE PRODUCTS; HIS-TAG; INCLUSION BODIES; NANO-LC; NICKEL COLUMN; OPEN READING FRAME; PEPTIDE ANALYSIS; POLYMERASE CHAIN REACTION; RECOMBINANT HUMAN INTERFERON; SDS-PAGE; SITE DIRECTED MUTAGENESIS; SODIUM DODECYL SULPHATE; THIOREDOXINS; TOTAL PROTEIN;

AMINES; ANTIBIOTICS; DIFFUSERS (OPTICAL); ELECTROPHORESIS; ENCODING (SYMBOLS); GENE ENCODING; GLYCOPROTEINS; INTERFERONS; MASS SPECTROMETRY; NUCLEIC ACIDS; OLIGONUCLEOTIDES; OPTIMIZATION; PURIFICATION; SODIUM;

ESCHERICHIA COLI;

PLASMID VECTOR; RECOMBINANT ALPHA2B INTERFERON; THIOREDOXIN;

ARTICLE; BASE PAIRING; CELL INCLUSION; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE SYNTHESIS; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN RENATURATION; RECOMBINANT GENE; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; THERMODYNAMICALLY BALANCED INSIDE OUT;

CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; INTERFERON ALFA-2B; OPEN READING FRAMES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 72949105337     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.11.008     Document Type: Article

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