Biological screening of a diverse set of AI-2 analogues in Vibrio harveyi suggests that receptors which are involved in synergistic agonism of AI-2 and analogues are promiscuous

Chemical Communications

Volumn , Issue 45, 2009, Pages 7033-7035

  Smith, Jacqueline A I ^a   Wang, Jingxin ^a   Nguyen Mau, Sao Mai ^a   Lee, Vincent ^a   Sintim, Herman O ^a  

^a University of Maryland   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AI 2 ANALOG; ALKYL GROUP; CHLORPROPHAM; DIHYDROPYRIMIDINE DEHYDROGENASE; RECEPTOR; UNCLASSIFIED DRUG;

ARTICLE; BIOLUMINESCENCE; CHEMICAL STRUCTURE; CONTROLLED STUDY; NONHUMAN; SCREENING; SYNTHESIS; VIBRIO HARVEYI;

EID: 72849147446     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b909666c     Document Type: Article

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22. We find that AI-2 prepared using our method is stable and biologically active after several months of storage, even at millimolar solutions in DMSO/water Although synergistic agonism assays of AI-2 analogues have been previously done without added boric acid (see ref. 13), we added boric acid (100 μM) to our assay. We thank an anonymous reviewer who pointed out that when boric acid is not added to the culture media for the bioluminescence assay, the analogues might scavenge for adventitious borate; thereby affecting the results of different assays that contained different amounts of adventitious borate. We nonetheless confirm Janda's report that AI-2 analogues cause fold enhancement of AI-2 induced bioluminescence in V. harveyi, although that study did not add boric acid to the media for the bioluminescence assay
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