Benghalensin, a highly stable serine protease from the latex of medicinal plant Ficus benghalensis

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 23, 2009, Pages 11120-11126

  Sharma, Anurag ^a   Kumari, Moni ^a   Jagannadham, M V ^a  

^a BANARAS HINDU UNIVERSITY   (India)

Author keywords

Benghalensin; Ficus benghalensis; Latex; Medicinal plant; Serine protease

Indexed keywords

LATEX; SERINE PROTEINASE; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; FICUS; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; MEDICINAL PLANT; METABOLISM; MOLECULAR WEIGHT;

ENZYME STABILITY; FICUS; ISOELECTRIC POINT; LATEX; MOLECULAR WEIGHT; PLANT PROTEINS; PLANTS, MEDICINAL; SERINE PROTEASES;

FICUS BENGHALENSIS;

EID: 72449194286     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf902279u     Document Type: Article

References (46)

1. Brady, C. J. Fruit ripening. Annu. Rev. Plant Physiol. 1985, 38, 155-178.
2. (2) Kembhavi, A. A.; Buttle, D. J.; Knight, C. G.; Barrett, A. J. The two cysteine endopeptidases of legume seeds: purification and characterization by use of specific fluorometric assays. Arch. Biochem. Biophys 1993, 303, 208-213. (Pubitemid 23199812)
3. Taylor, R. M.; Cuming, A. C. Purification of an endoproteinase that digests the wheat 'Em' protein in vitro, and determination of its cleavage sites. FEBS Lett. 1993, 27, 76-80.
4. Rudenskaya, G. N.; Bogacheva, A. M.; Preusser, A.; Kuznetsova, A. V.; Dunaevsky, Ya. E.; Golovkin, B. N.; et al. Taraxalisin-a serine proteinase from dandelion Taraxacum officinale Webb s.l. FEBS Lett. 1998, 437, 237-240.
5. Boller, T. Roles of proteolytic enzymes in interactions of plants with, other organisms. In Plant Proteolytic Enzymes; Dalling, M. J., Ed.; CRC Press: 1986; pp 67-96.
6. Smit, H. F.; Woerdenbag, H. J.; Singh, R. H.; Meulenbeld, G. J.; Labadie, R. P.; Zwaving, J. H. Ayurvedic herbal drugs with, possible cytostatic activity, J. Ethnopharmacol. 1995, 47, 75-84.
7. Zamost, B. L.; Nielsen, H. K.; Starnes, R. L. Thermostable enzymes for industrial applications. J. Ind. Microbiol. Biotechnol. 1991, 8, 71-82.
8. Tavaria, F. K.; Sousa, M, J.; Domingos, A.; Malcata, F. X.; Bordelius, P.; Clemente, A.; Paris, M. S. Degradation of caseins from milk of different species by extracts of Centaurea calcitrapa. J. Agric. Food Chem. 1997, 45, 3760-3765.
9. Pintado, A. I.; Macedo, A. C.; Teixeira, G.; Paris, M. S.; Clemente, A.; Malcata, F. X. Caseinolytic activity of fruit extract from Opuntia ficus-indica on bovine, caprine, and ovine sodium caseinates. Biotechnol Prog. 2001, 17, 643-646.
10. Giao, M. S.; Gonzalez-Sanjose, M. L.; Muniz, P.; Rivero-Perez, M. D.; Kosinska, M.; Pintado, M. E.; Malcata, F. X. Protection of deoxyribose and DNA from, degradation by using aqueous extracts of several wild plants. J. Sci. Food Agric. 2008, 88, 633-640.
11. Kundu, S.; Sundd, M.; Jagannadham, M. V. Purification and characterization of a stable cysteine protease ervatamin B, with two disulfide bridges, from the latex of Ervatamia coronaria, J. Agric. Food Chem. 2000, 48, 171-179.
12. Nallamsetty, S.; Kundu, S.; Jagannadham, M. V. Purification and biochemical characterization of a highly active cysteine protease Ervatamin A from the latex of Ervatamia coronaria, J. Protein Chem. 2003, 22, 1-13.
13. Patel, B. K.; Jagannadham, M. V. A high cysteine containing thiol proteinase from the latex of Ervatamia heyneana: purification and comparison with ervatamin B and C from Ervatamia coronaria. J. Agric. Food Chem. 2003, 51, 6326-6334.
14. Patel, A. K.; Singh, V. K.; Moir, A. J.; Jagannadham, M. V. Biochemical and spectroscopic characterization of morning glory peroxidase from an invasive and hallucinogenic plant weed Ipomoea carnea. J. Agric. Food Chem. 2008, 56, 9236-9245.
15. Glazer, A. N.; Smith, E. L. Papain and other plant sulfhydryl proteolytic enzymes. In The Enzymes; Boyer, P. D., Ed.; Academic Press:New York, 1971 ; Vol.3, pp 501 -546.
16. Robinson, G. W. Isolation and characterization of papaya peptidase A from commercial chymopapain, Biochemistry 1975, 14, 3695-3700.
17. Sgarbieri, V. C.; Gupte, S. M.,; Kramer, D. E.; Whitaker, J. R. Ficus enzymes I. Separation of the proteolytic enzymes of Fisus carica and Ficus glabrata latices. J. Biol. Chem. 1964, 239, 2170-2177.
18. Pande, M.; Dubey, V. K.; Yadav, S. C.; Jagannadham, M. V. A novel serine protease cryptolepain from. Cryptolepis buchanani: purification and biochemical characterization. J. Agric. Food Chem. 2006, 54, 10141-10150.
19. Patel, A. K.; Singh, V. K.; Jagannadham, M. V. Carnein, a serine protease from, noxious plant weed Ipomoea carnea (morning glory). J. Agric. Food Chem. 2007, 55, 5809-5818.
20. Tomar, R.; Kumar, R.; Jagannadham, M. V. A stable serine protease, wrightin, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: purification and biochemical properties. J. Agric. Food Chem. 2008, 56, 1479-1487.
21. Antao, C. M.; Malcata, F. X. Plant serine proteases: biochemical, physiological and molecular features. Plant Physiol. Biochem. 2005, 43, 637-650.
22. Kang, H.; Kim, Y. S.; Chung, G. C. Characterization of natural rubber biosynthesisin Ficus benghalensis. Plant Physiol. Biochem. 2000, 38, 979-987.
23. Khan, T.; Tatke, P.; Gabhe, S. Y. Immunological studies on the aerial roots of the Indian banyan, J. Pharm. Sci. 2008, 70, 287-291.
24. Aswar, M.; Aswar, U.; Wagh, A.; Watkar, B.; Vyas, M.; Gujar, K. N. Antimicrobial activity of ficus benghalensis. Pharmacologyonline 2008, 2, 715-725.
25. (25) Muthu, C.; Ayyanar, M.; Raja, N.; Ignacimuthu, S. Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu, India. J. Ethnobiol. Ethnomed. 2006, 2, 43. (Pubitemid 46979404)
26. Bradford, M. M. A rapid and sensitive method for quantitation of microgram quantities of protein, utilizing principle of protein-dye-binding, Anal. Biochem. 1976, 72, 248-254.
27. Arnon, R. Papain. Methods Enzymol. 1970, 19, 226-244.
28. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
29. Merril, C. R. Gel - stain techniques, Methods Enzymol. 1990, 182, 477-488.
30. Hounsell, E. F.; Davies, M. J.; Smith, K. D. Chemical methods of analysis of glycoprotein. In The Protein Protocols Handbook; Walker, J. M., Ed.; Humana Press: Totowa, NJ, 1997; pp 633- 634.
31. Zacharius, R. M.,; Morrison, T. E. Z. H.; Woodlock, J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal. Biochem. 1969, 30, 148-152.
32. Sarath, G.; da la Motte, R. S.; Wagner, F. W. Protease assay methods. In Proteolytic Enzymes - a Practical Approach; Beynon, R. J., Bond, J. S., Ed.; IRL Press: Oxford, 1989; pp 25-55.
33. Goodwin, W.; Morton, R. A. The spectrophotometric determination, of tyrosine and tryptophan in. proteins, Biochem. J. 1946, 40, 628-632.
34. Ellman, L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 1959, 82, 70-77.
35. Aitken, A.; Learmonth, M. Protein determination by UV absorption. In The Protein Protocol Handbook; Walker, J. M., Ed.; Humana Press: Totowa, NJ, 1997; pp 3-6.
36. (36) Shevchenko, A.; WiIm, M.; Vorm, O.; Mann, M. Mass spectrometric sequencing of proteins from silver-stained Polyacrylamide gels. Anal. Chem. 1996, 68, 850-858. (Pubitemid 26101214)
37. Yadav, S. C.; Jagannadham, M. V.; Kundu, S.; Jagannadham, M. V. A kinetically stable plant subtilase with unique peptide mass finger-prints and dimerization properties, Biophys. Chem. 2009, 139, 13-23.
38. Lynn, K. R.; Clevette Radford, N. A. Two proteases from, the latex of Elaeophorbia drupifera. Phytochemistry 1985, 24, 2843-2845.
39. Yadav, S. C.; Pande, M.; Jagannadham, M. V. Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii. Phytochemistry 2006, 67, 1414-1426.
40. Moro, L. P.; Murakami, M. T.; Cabral, H.; Vidotto, A.; Tajara, E. H.,; Ami, R. K.; et al. Purification, Biochemical and functional characterization of miliin, a new thiol dependent serine protease isolated from the latex of Euphorbia milii. Protein Pept. Lett 2008, 15, 724-730.
41. Uchikoba, T.; Yonezawa, H.; Kaneda, M. Cleavage specificity of cucumisin, a plant serine protease. J. Biochem. 1995, 117, 1126-1130.
42. Singh, V.; Patel, A. K.; Moir, A. J.; Jagannadham, M. V. Indicain, a dimeric serine protease from Morus indica cv. K2. Phytochemistry 2008, 69, 2110-2119.
43. Uchikoba, T.; Horita, H.; Kaneda, M. Proteases of the sarcocarp of yellow snake-guard, Phytochemistry 1990, 29, 1879-1881.
44. Curroto, E.; Gonzalez, G.; O'Reilly, S.; Tapia, G. Isolation and partial characterization of a protease from Cucurbita ficifolia. FEBS Lett. 1989, 243, 363-365.
45. Yamagata, H.; Ueno, S.; Iwasaki, T. Isolation and characterization, of a possible native cucumisin from developing melon fruits and its limited autolysis to cucumisin. Agric. Biol. Chem. 1989, 53, 1009-1017.
46. Terp, N.; Thomsen, K. K.; Svendsen, I.; Davy, A.; Simpson, D. J. Purification, and characterization of hordolisin, a subtilisin-like serine endoprotease from barley. J. Plant. Physiol. 2000, 156, 468-476.