Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii

Protein and Peptide Letters

Volumn 15, Issue 7, 2008, Pages 724-730

  Moro, L P ^a   Murakami, M T ^b   Cabral, H ^c   Vidotto, A ^d   Tajara, E H ^d   Arni, R K ^a   Juliano, L ^e   Bonilla Rodriguez, G O ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b Brazilian Synchrotron Light Laboratory   (Brazil)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

^d FACULDADE DE MEDICINA DE SÃO JOSÉ DO RIO PRETO   (Brazil)

^e FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Characterization; Euphorbia milii; Latex; Medicinal plant; Purification; Serine protease

Indexed keywords

LATEX; SERINE PROTEINASE;

ARTICLE; CHEMISTRY; ENZYMOLOGY; EUPHORBIA; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; EUPHORBIA; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; KINETICS; LATEX; MOLECULAR WEIGHT; SERINE ENDOPEPTIDASES; TEMPERATURE;

EUPHORBIA; EUPHORBIA MILII;

EID: 49449115536     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986608785133744     Document Type: Article

References (48)

1. Vallés, D., Furtado, S., Cantera, A.M.B. (2007) Enzyme Microb. Technol., 40, 409-413.
2. Kaneda, M., Yonezawa, H., Uchikoba, T. (1997) Biosci. Biotechnol. Biochem., 61, 2100-2102.
3. Sundd, M., Kundu, S., Pal, G.P., Medicherla, J.V. (1998) Biosci. Biotechnol. Biochem., 62, 1947-1955.
4. Kundu, S., Sundd, M., Jagannadham, M.V. (2000) J. Agric. Food Chem., 48, 171-179.
5. Dubey, V.K., Jagannadham, M.V. (2003). Phytochemistry, 62, 1057-1071.
6. Patel, B.K., Jagannadham, M.V. (2003) J. Agric. Food Chem., 51, 6326-6334.
7. Brady, C.J. (1985). Fruit ripening. Ann. Rev. Plant Physiol., 38, 155-178.
8. Kembhavi, A.A., Buttle, D.J., Knight, C.G., Barrett, A.J. (1993) Arch. Biochem. Biophys., 303, 208-213.
9. Taylor, R.M., Cuming, A.C. (1993) FEBS Lett., 331, 76-80.
10. Rudenskaya, G.N., Bogacheva, A.M., Preusser, A., Kuznetsova, A.V., Dunaevsky, Ya.E., Golovkin, B.N., Stepanov, V.M. (1998) FEBS Lett., 473, 237-240.
11. Boller, T. (1986) In: Dalling, M. J. Plant Proteolytic Enzymes, pp. 67-95.
12. Smith, H.F., Woerdenbag, H.J., Singh, R.H., Meulenbeld, G.J., Labadie, R.P., Zwaving, J.H. (1995) J. Ethnopharmacol., 47, 75-84.
13. Vierstra, R.D. (1996) Plant Mol. Biol., 32, 275-302.
14. Vogg, G.; Mattes, E.; Polack, A.; Sandermann, H. Jr. (1999) Environ. Health Perspect., 107, 753-756.
15. Vasconcellos, M. C.; Schall, V. T. (1986) Memórias do Instituto Oswaldo Cruz, 81, 475-476.
16. Pile, E., Santos, J. A. A., São Luiz, J. B., Vasconcellos, M.C. (2001) Braz. J. vet. Res. Anim. Sci., 38, 288-289.
17. Lee, K.-H., Hayashi, N., Okano, M., Hall, I.R., Wu, R.-Y., Mcphail, A.T. (1982) Phytochemistry, 21, 1119-1121.
18. Delgado, I.F., De Carvalho, R.R., De Oliveira A.C.A.X.; Kuriyama, S. N.; Oliveira-Filho, E. C.; Souza, C. A. M.; Paumgartten, F. J. R. (2003) Toxicology Letts., 145, 175-180.
19. Bradford, M.M. (1976) Anal. Biochem., 72, 248-254.
20. Cabral, H., Leopoldino, A. M., Silva, E. H. T., Greene, L. J., Faça, V.M., Mateus, R. P., Ceron, C. R., Judice, W.A.S., Juliano, L., Bonilla-Rodriguez, G.O. (2006) Protein Peptide Letts., 13, 83-89.
21. Sequeiros, C., López, L. M.I., Caffini, N.O., Natalucci, C.L. (2003) Fitoterapia, 74, 570-577.
22. Laemmli, U.K. (1970) Nature, 227, 680-685.
23. García-Carreño, F.L., Dimes, L.E., Haard, N.F. (1993) Analy. Biochem., 214, 65-69.
24. Dunn, BM (1996) In: Beynon, R.J. and Bond, J.S. Proteolytic enzymes: A practical approach. Pp. 57-82. IRL Press, Oxford.
25. Bjellqvist, B., Ek K, Righetti PG, Gianazza E, Gorg A, Westermeier R, Postel W. (1982) J. Biochem.Biophys., 6, 317-339.
26. Görg, A., Postel, A., Günther, S. (1988) Electrophoresis, 9, 531-546.
27. Görg, A., Obemaier, C., Boguth, G., Harder, A., Scheibe, B., Wildgruber, R., Weiss, W. (2000) Electrophoresis, 21, 1037-1053.
28. Trovó-Marqui, A. B.; Vidotto A., Polachini G. M., Bellato, C. M., Cabral, H., Leopoldino, A. M., Fukuyama, E. E., Cury, P. M., Palma, M. S., Tajara, E. H. (2006) J. Biochem. Mol. Biol., 39, 216-222.
29. Yadav, SC., Pande, M., Jagannadham MV. (2006) Phytochemistry, 67, 1414-1426.
30. Arima, K., Uchikoba, T., Yonezawa, H., Shimada, M., Kaneda, M. (2000) Phytochemistry, 53, 639-644.
31. Asif-Ullah,-M; Kim,-KS; Yu,-YG. (2006) Phytochemistry, 67, 870-5.
32. Pande, M., Dubey, V. K., Yadav, S. C., and Jagannadham, M. V. (2006) J. Agric. Food Chem., 54, 10141-10150.
33. James, F.; Brouquisse, R.; Suire, C.; Pradet, A.; Raymond, P. (1996) Biochem. J, 320, 283-292.
34. Lynn, K.R., Clevette-Radford, N.A. (1988) Phytochemistry, 27, 45-50.
35. Antão, C. M., Malcata, M. X. (2005) Plant Physiol. Biochem., 43, 637-650.
36. Sokolova, E.A., Starkova, N.N., Vorotyntsera, T., Zamolodchikova, T.S. (1998) Eur. J. Biochem., 225, 501-507.
37. Drapeau, G.R.; Boily, Y.; Houmard, J. (1972) J. Biol. Chem., 247, 6720-6726.
38. Shannon, J. D., Wallace, W. (1979) Eur. J. Biochem., 102, 399-408.
39. Nallamsetty, S., Kundu, S., Jagannadham, M.V. (2003) J. Prot. Chem., 22, 1-13.
40. Savelli G, Spreti N, Di Profio P. (2000) Curr. Opin. Coll. Inter. Sci., 5, 111-117.
41. Uchikoba, T., Yonezawa, H., Kaneda, M. (1995) J. Biochem., 117, 1126-1130.
42. Patel, A.K., Singh, V.K., and Jagannadham, M.V. (2007) J. Agric. Food Chem., 10, 1021.
43. Yamagata, H., Ueno, S., Iwasaki, T. (1989) Agric. Biol. Chem., 53, 1009-1017.
44. Curroto, E., Gonzalez, G., O'Reilly, S., Tapia, G. (1989) FEBS Lett., 243, 363-365.
45. Uchikoba, T., Yonezawa, H., Kaneda, M., (1998) Phytochemistry, 49, 2215-2219.
46. Lynn, K.R., Clevette-Radford, N.A. (1984) Phytochemistry, 3, 682-683.
47. Uchikoba, T., Horita, H., Kaneda, M. (1990) Phytochemistry, 29, 1879-1881.
48. Erlanger, B.F., Kokowsky, N., Cohen, W. (1961) Arch. Biochem. Biophys., 95, 271-275.