Lysine racemase: A novel non-antibiotic selectable marker for plant transformation

Plant Molecular Biology

Volumn 72, Issue 1-2, 2010, Pages 153-169

  Chen, I Chieh ^a   Thiruvengadam, Venkatesan ^a   Lin, Wei De ^a,b   Chang, Ho Hsiung ^a   Hsu, Wen Hwei ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b CHINA MEDICAL UNIVERSITY HOSPITAL   (Taiwan)

Author keywords

Arabidopsis thaliana; L lysine; Lysine racemase; Non antibiotic selectable marker; Plant transformation; Tobacco

Indexed keywords

ISOMERASE; LYSINE; LYSINE RACEMASE;

ARABIDOPSIS; ARTICLE; BIOLOGICAL MODEL; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; METABOLISM; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TOBACCO; TRANSGENIC PLANT; WESTERN BLOTTING;

AMINO ACID ISOMERASES; ARABIDOPSIS; BLOTTING, SOUTHERN; BLOTTING, WESTERN; LYSINE; MODELS, GENETIC; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; TOBACCO; TRANSFORMATION, GENETIC;

ARABIDOPSIS; ARABIDOPSIS THALIANA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 72149119473     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-009-9558-y     Document Type: Article

References (80)

1. ACNFP (1994) Report on the use of antibiotic resistance markers in genetically modified food organisms. Advisory Committee on Novel Foods and Processes. Department of Health and Ministry of Agriculture, Fisheries and Food, London
2. P Arruda SW Bright JSH Kueh PJ Lea SE Rognes 1984 Regulation of aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine Plant Physiol 76 442 446 10.1104/pp.76.2.442 10.1104/pp.76.2.442 1:CAS:528: DyaL2MXitFeltQ%3D%3D 16663861
3. N Arumugam V Gupta A Jagannath A Mukhopadhyay AK Pradhan PK Burma, et al. 2007 A passage through in vitro culture leads to efficient production of marker-free transgenic plants in Brassica juncea using the Cre-loxP system Transgenic Res 16 703 712 10.1007/s11248-006-9058-7 10.1007/s11248-006-9058-7 1:CAS:528:DC%2BD2sXht1Kgs7bI 17219247 (Pubitemid 350060753)
4. KS Blundy MAC Blundy D Carter F Wilson WD Park MM Burrell 1991 The expression of class I patatin gene fusions in transgenic potato varies with both gene and cultivar Plant Mol Biol 16 153 160 10.1007/BF00017925 10.1007/BF00017925 1:CAS:528:DyaK3MXhtFKiu78%3D 1888893
5. KJ Boorer WB Frommer DR Bush M Kreman DDF Loo EM Wright 1996 Kinetics and specificity of a H+/amino acid transporter from Arabidopsis thaliana J Biol Chem 271 2213 2220 10.1074/jbc.271.4.2213 10.1074/jbc.271.4.2213 1:CAS:528:DyaK28Xns1Sjsw%3D%3D 8567681 (Pubitemid 26047813)
6. DC Boyes AM Zayed R Ascenzi AJ McCaskill NE Hoffman KR Davis, et al. 2001 Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants Plant Cell 13 1499 1510 10.1105/tpc.13.7.1499 10.1105/tpc.13.7.1499 1:CAS:528:DC%2BD3MXlsFKltrk%3D 11449047 (Pubitemid 32675716)
7. Bryan JK (1980) Synthesis of the aspartate family and branched chain amino acids. In: Miflin BJ (ed) The biochemistry of plants, vol 5. Academic Press, New York, pp 403-452
8. YL Chan KH Lin Sanjaya LJ Liao WH Chen MT Chan 2005 Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack Transgenic Res 14 279 288 10.1007/s11248-005-0106-5 10.1007/s11248-005-0106-5 1:CAS:528:DC%2BD2MXlvFOgtbg%3D 16145836 (Pubitemid 40968370)
9. PY Chen CK Wang SC Soong KY To 2003 Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants Mol Breed 11 287 293 10.1023/A:1023475710642 10.1023/A:1023475710642 1:CAS:528:DC%2BD3sXjtFKgtbg%3D (Pubitemid 36590376)
10. IC Chen WD Lin SK Hsu V Thiruvengadam WH Hsu 2009 Isolation and characterization of a novel lysine racemase from a soil metagenomic library Appl Environ Microbiol 75 5161 5166 10.1128/AEM.00074-09 10.1128/AEM.00074-09 1:CAS:528:DC%2BD1MXhtVSgtrnO 19502445
11. HJ Cho JE Brotherton JM Widholm 2004 Use of the tobacco feedback-insensitive anthranilate synthase gene (ASA2) as a selectable marker for legume hairy root transformation Plant Cell Rep 23 104 113 10.1007/s00299-004-0789-8 10.1007/s00299-004-0789-8 1:CAS:528: DC%2BD2cXmtFWlu7s%3D 15168071
12. SJ Clough AF Bent 1998 Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Plant J 16 735 743 10.1046/j.1365-313x.1998.00343.x 10.1046/j.1365-313x.1998.00343.x 1:STN:280:DyaK1M7mvVagsQ%3D%3D 10069079 (Pubitemid 29083518)
13. O Cotsaftis C Sallaud JC Breitler D Meynard R Greco A Pereira, et al. 2002 Transposon-mediated generation of T-DNA- and marker-free rice plants expressing a Bt endotoxin gene Mol Breed 10 165 180 10.1023/A:1020380305904 10.1023/A:1020380305904 1:CAS:528:DC%2BD38Xnt1ahtLk%3D (Pubitemid 35181297)
14. W Cuellar A Gaudin D Solórzano A Casas L Nopo P Chudalayandi, et al. 2006 Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato Plant Mol Biol 62 71 82 10.1007/s11103-006-9004-3 10.1007/s11103-006-9004-3 1:CAS:528: DC%2BD28XptlSmt74%3D 16912912 (Pubitemid 44372888)
15. EC Dale DW Ow 1991 Gene transfer with subsequent removal of the selection gene from the host genome Proc Natl Acad Sci USA 88 10558 10562 10.1073/pnas.88.23.10558 10.1073/pnas.88.23.10558 1:CAS:528:DyaK38XjsFaqsQ%3D%3D 1660141
16. H Daniell B Muthukumar SB Lee 2001 Marker free transgenic plants: engineering the chloroplast genome without use of antibiotic selection Curr Genet 39 109 116 10.1007/s002940100185 10.1007/s002940100185 1:CAS:528:DC%2BD3MXivFais7w%3D 11405095 (Pubitemid 32422020)
17. B Darbani A Eimanifar CN Stewart Jr WN Camargo 2007 Methods to produce marker-free transgenic plants Biotechnol J 2 83 90 10.1002/biot.200600182 10.1002/biot.200600182 1:CAS:528:DC%2BD2sXit1Wmu7o%3D 17167767 (Pubitemid 46738890)
18. M De Block D De Brower P Tenning 1989 Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants Plant Physiol 91 694 701 10.1104/pp.91.2.694 10.1104/pp.91.2.694 16667089
19. N de Vetten AM Wolters K Raemakers I van der Meer R ter Stege E Heeres, et al. 2003 A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop Nat Biotechnol 21 439 442 10.1038/nbt801 10.1038/nbt801 12627169 (Pubitemid 36397666)
20. C Dean J Jones M Favreau JP Dunsmuir J Bedbrook 1988 Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants Nucleic Acids Res 16 9267 9283 10.1093/nar/16.19.9267 10.1093/nar/16.19.9267 1:CAS:528:DyaL1cXmt1ers7w%3D 3174450
21. JJ Doyle JL Doyle 1990 Isolation of plant DNA from fresh tissue Focus 12 13 15
22. H Ebinuma K Sugita E Matsunaga M Yamakado 1997 Selection of marker-free transgenic plants using the isopentenyl transferase gene Proc Natl Acad Sci USA 94 2117 2121 10.1073/pnas.94.6.2117 10.1073/pnas.94.6.2117 1:CAS:528: DyaK2sXhvF2ltrk%3D 11038607 (Pubitemid 27136837)
23. H Ebinuma K Sugita E Matsunaga S Endo K Yamada A Komamine 2001 Systems for the removal of a selection marker and their combination with a positive marker Plant Cell Rep 20 383 392 10.1007/s002990100344 10.1007/s002990100344 1:CAS:528:DC%2BD3MXltFOhurw%3D (Pubitemid 32696181)
24. A Ebmeier L Allison H Cerutti T Clemente 2004 Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation Planta 218 751 758 10.1007/s00425-003-1129-x 10.1007/s00425-003- 1129-x 1:CAS:528:DC%2BD2cXitFaht7w%3D 14673650 (Pubitemid 38390494)
25. S Endo K Sugita M Sakai H Tanaka H Ebinuma 2002 Single-step transformation for generating marker-free transgenic rice using the ipt-type MAT vector system Plant J 30 115 122 10.1046/j.1365-313X.2002.01272.x 10.1046/j.1365-313X.2002.01272.x 1:CAS:528:DC%2BD38XjvFGgs78%3D 11967098 (Pubitemid 34439704)
26. O Erikson M Hertzberg T Nasholm 2004 A conditional marker gene allowing both positive and negative selection in plants Nat Biotechnol 22 455 458 10.1038/nbt946 10.1038/nbt946 1:CAS:528:DC%2BD2cXis1yjtr0%3D 15085802 (Pubitemid 38451378)
27. O Erikson M Hertzberg T Nasholm 2005 The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation Plant Mol Biol 57 425 433 10.1007/s11103-004-7902-9 10.1007/s11103-004-7902-9 1:CAS:528: DC%2BD2MXjt1Wgtrk%3D 15830131 (Pubitemid 40572922)
28. RT Fraley SG Rogers RB Horsch PR Sanders JS Flick SP Adams, et al. 1983 Expression of bacterial genes in plant cells Proc Natl Acad Sci USA 80 4803 4807 10.1073/pnas.80.15.4803 1:CAS:528:DyaL3sXltFOisb4%3D 6308651
29. WB Frommer S Hummel M Unseld O Ninnemann 1995 Seed and vascular expression of a high-affinity transporter for cationic amino acids in Arabidopsis Proc Natl Acad Sci USA 92 12036 12040 10.1073/pnas.92.26.12036 1:CAS:528:DyaK28Xhtlyqtg%3D%3D 8618839 (Pubitemid 26014006)
30. KZ Gamburg NI Rekoslavskaya 1991 Formation and function of D-amino acids in plants Soviet Plant Physiol 38 904 912
31. AP Gleave DS Mitra SR Mudge BAM Morris 1999 Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene Plant Mol Biol 40 223 235 10.1023/A:1006184221051 10.1023/A:1006184221051 1:CAS:528:DyaK1MXkvVygsLw%3D 10412902 (Pubitemid 29294162)
32. AP Goldbrough CN Lastrella JI Yoder 1993 Transposition mediated repositioning and subsequent elimination of marker genes from transgenic tomato Bio/Tech 11 1286 1292 10.1038/nbt1193-1286
33. A Haldrup S Petersen F Okkels 1998 The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xylose as the selection agent Plant Mol Biol 37 287 296 10.1023/A:1005910417789 10.1023/A:1005910417789 1:CAS:528: DyaK1cXjtlalsLw%3D 9617801 (Pubitemid 28219250)
34. G Hansen MS Wright 1999 Recent advances in the transformation of plants Trends Plant Sci 4 226 231 10.1016/S1360-1385(99)01412-0 10.1016/S1360-1385(99) 01412-0 10366879 (Pubitemid 29270617)
35. TTC Hoa BB Bong E Huq TK Hodges 2002 Cre/lox site-specific recombination controls the excision of a transgene from the rice genome Theor Appl Genet 104 518 525 10.1007/s001220100748 10.1007/s001220100748 1:CAS:528: DC%2BD38XjtlSqtrc%3D 12582653 (Pubitemid 36080380)
36. R Höfgen L Willmitzer 1988 Storage of competent cells for Agrobacterium transformation Nucleic Acids Res 16 9877 10.1093/nar/16.20.9877 10.1093/nar/16.20.9877 3186459
37. RB Horsch JE Fry NL Hoffmann D Eichholtz SG Rogers RT Fraley 1985 A simple and general method for transferring genes into plants Science 227 1229 1231 10.1126/science.227.4691.1229 10.1126/science.227.4691.1229 1:CAS:528:DyaL2MXhtFSjsLc%3D
38. P Hsiao Sanjaya RC Su JA Teixeira da Silva MT Chan 2007 Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation Planta 225 897 906 10.1007/s00425-006-0405-y 10.1007/s00425-006- 0405-y 1:CAS:528:DC%2BD2sXhvVeisro%3D 17039373 (Pubitemid 46317972)
39. HT Huang JW Davisson 1958 Distribution of lysine racemase in bacteria J Bacteriol 76 495 498 1:CAS:528:DyaG1MXhsl2jsA%3D%3D 13598707
40. M Joersbo FT Okkels 1996 A novel principle for selection of transgenic plant cells: positive selection Plant Cell Rep 16 219 221 10.1007/s002990050210 10.1007/BF01890871 1:CAS:528:DyaK2sXotVSltw%3D%3D (Pubitemid 27028165)
41. M Joersbo I Donaldson J Kreiberg SG Petersen J Brunstedt F Okkels 1998 Analysis of mannose selection used for transformation of sugar beet Mol Breed 4 111 117 10.1023/A:1009633809610 10.1023/A:1009633809610 1:CAS:528: DyaK1cXjtFeiuro%3D (Pubitemid 28209528)
42. M Joersbo K Joregensen J Brunstedt 2003 A selection system for transgenic plants based on galactose as selective agent and a UDP-glucose: galactose-1-phosphate uridyltransferase gene as selective gene Mol Breed 11 315 323 10.1023/A:1023402424215 10.1023/A:1023402424215 1:CAS:528: DC%2BD3sXjtFKgtbc%3D (Pubitemid 36590379)
43. S Kerbach H Lörz D Becker 2005 Site-specific recombination in Zea mays Theor Appl Genet 111 1608 1616 10.1007/s00122-005-0092-2 10.1007/s00122-005-0092-2 1:CAS:528:DC%2BD2MXht1SqtLnO 16200415 (Pubitemid 41618662)
44. SMJ Klaus FC Huang TJ Golds HU Koop 2004 Generation of marker-free plasmid transformants using a transiently cointegrated selection gene Nat Biotechnol 22 225 229 10.1038/nbt933 10.1038/nbt933 1:CAS:528: DC%2BD2cXnvFeiug%3D%3D 14730316 (Pubitemid 38160540)
45. T Komari Y Hiei Y Saito N Murai T Kumashiro 1996 Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers Plant J 10 165 174 10.1046/j.1365-313X.1996.10010165.x 10.1046/j.1365-313X.1996. 10010165.x 1:CAS:528:DyaK28XltVamtrk%3D 8758986
46. T Kunkel QW Niu YS Chan NH Chua 1999 Inducible isopentenyl transferase as a high-efficiency marker for plant transformation Nat Biotechnol 17 916 919 10.1038/12914 10.1038/12914 1:CAS:528:DyaK1MXlslOmurg%3D 10471937 (Pubitemid 29416669)
47. I Kunze M Ebneth U Heim M Geiger U Sonnewald K Herbers 2001 2-Deoxyglucose resistance: a novel selection marker for plant transformation Mol Breed 7 221 227 10.1023/A:1011307508252 10.1023/A:1011307508252 1:CAS:528:DC%2BD3MXmtVGlu7c%3D (Pubitemid 32675681)
48. PR LaFayette PM Kane BH Phan WA Parrott 2005 Arabitol dehydrogenase as a selectable marker for rice Plant Cell Rep 24 596 602 10.1007/s00299-005-0015-3 10.1007/s00299-005-0015-3 1:CAS:528:DC%2BD2MXht1Wqur7L 16151815 (Pubitemid 41635985)
49. B Leyman N Avonce M Ramon PV Dijck G Iturriaga JM Thevelein 2006 Trehalose-6-phosphate synthase as an intrinsic selection marker for plant transformation J Biotechnol 121 3 309 317 10.1016/j.jbiotec.2005.08.033 10.1016/j.jbiotec.2005.08.033 1:CAS:528:DC%2BD28XlvVGjsA%3D%3D 16271790 (Pubitemid 43113103)
50. Z Li A Xing BP Moon SA Burgoyne AD Guida H Liang, et al. 2007 A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants Plant Mol Biol 65 329 341 10.1007/s11103-007- 9223-2 10.1007/s11103-007-9223-2 1:CAS:528:DC%2BD2sXhtVeisLrJ 17712602 (Pubitemid 47414563)
51. B Li C Xie H Qiu 2009 Production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency Plant Cell Rep 28 373 386 10.1007/s00299-008-0640-8 10.1007/s00299-008-0640-8 1:CAS:528:DC%2BD1MXitlSqtL4%3D 19018535
52. WD Lin JY Wu CC Lai FJ Tsai CH Tsai SP Lin, et al. 2001 A pilot study of neonatal screening by electrospray ionization tandem mass spectrometry in Taiwan Acta Paediatr Taiwan 42 224 230 1:STN:280:DC%2BD3MrgtFeruw%3D%3D 11550411
53. K Lindsey P Gallois 1990 Transformation of sugarbeet (Beta vulgaris) by Agrobacterium tumefaciens J Exp Bot 41 529 536 10.1093/jxb/41.5.529 10.1093/jxb/41.5.529 1:CAS:528:DyaK3cXlvVGqur0%3D
54. K Luo Z Xuelian C Yongqin X Yuehua Z Degang M Richard, et al. 2006 The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium-mediated tobacco transformation Plant Cell Rep 25 403 409 10.1007/s00299-005-0051-z 10.1007/s00299-005-0051-z 1:CAS:528: DC%2BD28Xjs1KksrY%3D 16369767 (Pubitemid 43193147)
55. G Marjanac A De Paepe I Peck A Jacobs S De Buck A Depicker 2008 Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana Transgenic Res 17 239 250 10.1007/s11248-007-9096-9 10.1007/s11248-007-9096-9 1:CAS:528:DC%2BD1cXjsVSks78%3D 17541719
56. A Mentewab CN Stewart Jr 2005 Overexpression of an Arabidopsis thaliana ABC transporter confers kanamycin resistance to transgenic plants Nat Biotechnol 23 1177 1180 10.1038/nbt1134 10.1038/nbt1134 1:CAS:528:DC%2BD2MXpvVyrtr4%3D 16116418 (Pubitemid 41486399)
57. S Miao DR Duncan JM Widholm 1988 Selection of regenerable maize callus cultures resistant to 5-methyl-DL-tryptophane, S-2-aminoethyl-L-cysteine and high levels of l-lysine plus l-threonine Plant Cell Tissue Org Cult 14 3 14 10.1007/BF00029570 10.1007/BF00029570 1:CAS:528:DyaL1cXlt1Kjs7g%3D
58. B Miki S McHugh 2004 Selectable marker genes in transgenic plants: applications, alternatives and biosafety J Biotechnol 107 193 232 10.1016/j.jbiotec.2003.10.011 10.1016/j.jbiotec.2003.10.011 1:CAS:528: DC%2BD2cXltVGrtg%3D%3D 14736458 (Pubitemid 38094321)
59. B Miki A Abdeen Y Manabe P MacDonald 2009 Selectable marker genes and unintended changes to the plant transcriptome Plant Biotechnol J 7 211 218 10.1111/j.1467-7652.2009.00400.x 10.1111/j.1467-7652.2009.00400.x 1:CAS:528:DC%2BD1MXkslGqurg%3D 19261135
60. L Mlynárová A Loonen J Heldens RC Jansen P Keizer WJ Stiekema, et al. 1994 Reduced position effect in mature transgenic plants conferred by the chicken lysozyme matrix-associated region Plant Cell 6 417 426 10.1105/tpc.6.3.417 10.1105/tpc.6.3.417 12244242 (Pubitemid 2070942)
61. T Murashige F Skoog 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol Plant 15 473 497 10.1111/j.1399-3054.1962. tb08052.x 10.1111/j.1399-3054.1962.tb08052.x 1:CAS:528:DyaF3sXksFKm
62. C Peach J Velten 1991 Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters Plant Mol Biol 17 49 60 10.1007/BF00036805 10.1007/BF00036805 1:CAS:528: DyaK3MXlvF2qu7Y%3D 1907871
63. A Perl S Galili O Shaul I Ben-Tzvi G Galili 1993 Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: two novel selectable markers for plant transformation Bio/Tech 11 715 718 10.1038/nbt0693-715 10.1038/nbt0693-715 1:CAS:528:DyaK3sXks1Chtbw%3D (Pubitemid 23164200)
64. M Rashed PT Ozand MP Bucknall D Little 1995 Diagnosis of inborn errors of metabolism from blood spots by acylcarnitines and aminoacids profiling using automated electrospray tandem mass spectrometry Pediatr Res 38 324 331 1:CAS:528:DyaK2MXosVChs70%3D 7494654
65. SE Rognes WJ Simon BJ Miflin 1983 Feedback-insensitive aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine Planta 157 32 38 10.1007/BF00394537 10.1007/BF00394537 1:CAS:528:DyaL3sXht1aqtb4%3D
66. CM Rommens 2004 All-native DNA transformation: a new approach to plant genetic engineering Trends Plant Sci 9 1360 1385 10.1016/j.tplants.2004.07.001 10.1016/j.tplants.2004.07.001 (Pubitemid 39160942)
67. D Rosellini S Capomaccio N Ferradini MLS Sardaro A Nicolia F Veronesi 2007 Non-antibiotic, efficient selection for alfalfa genetic engineering Plant Cell Rep 26 1035 1044 10.1007/s00299-007-0321-z 10.1007/s00299-007-0321-z 1:CAS:528:DC%2BD2sXnsFaqtbo%3D 17333020 (Pubitemid 350092182)
68. SH Russell JL Hoopes JT Odell 1992 Directed excision of a transgene from the plant genome Mol Gen Genet 234 49 59 10.1007/BF00272344 1:CAS:528: DyaK38XlsVCmsbs%3D 1495484
69. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
70. CP Scutt E Zubko P Meyer 2002 Techniques for the removal of marker genes from transgenic plants Biochimie 84 1119 1126 10.1016/S0300-9084(02)00021-4 10.1016/S0300-9084(02)00021-4 1:CAS:528:DC%2BD3sXht1Kns7o%3D 12595140 (Pubitemid 36253737)
71. RA Steinberg 1951 Amino acid toxicities to tobacco varieties differing in resistance to black root-rot Bull Torrey Bot Club 78 227 232 10.2307/2481978 1:CAS:528:DyaG3MXktFyrsQ%3D%3D
72. Tian L, Jordan M, Miki B (2006) Markers and selector genes for plant transformation. In: Teixeira da Silva JA (ed) Ornamental and plant biotechnology, vol II. Global Science Books, London, pp 9-20
73. S Tokuyama K Hatano 1995 Purification and properties of thermostable N-acylamino acid racemase from Amycolatopsis sp. TA-1-60 Appl Microbiol Biotechnol 42 853 859 10.1007/BF00191181 10.1007/BF00191181 1:CAS:528: DyaK2MXkvV2jt70%3D 7766084
74. C Waldron EB Murphy JL Roberts GD Gustafson SL Armour SK Malcolm 1985 Resistance to hygromycin B Plant Mol Biol 5 103 108 10.1007/BF00020092 10.1007/BF00020092 1:CAS:528:DyaL28Xhs1Cq
75. SJ You CH Liau HE Huang TY Feng V Prasad HH Hsiao, et al. 2003 Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation Planta 217 60 65 10.1007/s00425-002-0970-7 1:CAS:528:DC%2BD3sXjt1Omtrw%3D 12721849 (Pubitemid 36761686)
76. W Zhang S Subbarao P Addae A Shen C Armstrong V Peschke, et al. 2003 Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants Theor Appl Genet 107 1157 1168 10.1007/s00122-003-1368-z 10.1007/s00122-003- 1368-z 1:CAS:528:DC%2BD3sXos1Oktbc%3D 14513214 (Pubitemid 37424992)
77. Y Zhang H Li B Ouyang Y Lu Z Ye 2006 Chemical-induced autoexcision of selectable markers in elite tomato plants transformed with a gene conferring resistance to lepidopteran insects Biotechnol Lett 28 1247 1253 10.1007/s10529-006-9081-z 10.1007/s10529-006-9081-z 1:CAS:528: DC%2BD28XmvFeisL8%3D 16802102 (Pubitemid 44078493)
78. WJ Zhang SS Yang XY Shen YS Jin HJ Zhao T Wang 2009 The salt-tolerance gene rstB can be used as a selectable marker in plant genetic transformation Mol Breed 23 269 277 10.1007/s11032-008-9231-1 10.1007/s11032-008-9231-1
79. E Zubko C Scutt P Meyer 2000 Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes Nat Biotechnol 18 442 445 10.1038/74515 10.1038/74515 1:CAS:528:DC%2BD3cXis1Glsrw%3D 10748528 (Pubitemid 30217535)
80. J Zuo QW Niu SG Møller NH Chua 2001 Chemical-regulated, site-specific DNA excision in transgenic plants Nat Biotechnol 19 157 161 10.1038/84428 10.1038/84428 1:CAS:528:DC%2BD3MXhtVaitLg%3D 11175731 (Pubitemid 32144445)