A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants

Plant Molecular Biology

Volumn 65, Issue 3, 2007, Pages 329-341

  Li, Zhongsen ^a   Xing, Aiqiu ^a   Moon, Bryan P ^a   Burgoyne, Susan A ^a   Guida, Anthony D ^a   Liang, Huiling ^a   Lee, Catharina ^a   Caster, Cheryl S ^a   Barton, Joanne E ^a   Klein, Theodore M ^a   Falco, Saverio C ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Cre loxP recombination system; Marker gene free transgenic soybean; Self activating gene excision

Indexed keywords

BACTERIOPHAGES; ENZYME ACTIVITY; GENETIC ENGINEERING; PLANTS (BOTANY);

FREE TRANSGENIC SOYBEAN PLANTS; MARKER GENE; TRANSGENIC PLANTS;

GENES;

BETA GLUCURONIDASE; CRE RECOMBINASE; DRUG DERIVATIVE; GLYCINE; GLYPHOSATE; INTEGRASE;

ARABIDOPSIS; ARTICLE; BIOLOGICAL MODEL; DRUG EFFECT; GENE; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; SOUTHERN BLOTTING; SOYBEAN; TRANSGENIC PLANT; WESTERN BLOTTING;

ARABIDOPSIS; BASE SEQUENCE; BLOTTING, SOUTHERN; BLOTTING, WESTERN; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GLUCURONIDASE; GLYCINE; INTEGRASES; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS (GENETICS); SOYBEANS;

ARABIDOPSIS; GLYCINE MAX;

EID: 34548673290     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-007-9223-2     Document Type: Article

References (41)

1. Bayley CC, Morgan M, Dale EC, Ow DW (1992) Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site specific recombination system. Plant Mol Biol 18:353-361
2. Bowen BA, Bruce WB, Lu G, Sims LE, Tagliani LA (2003) Synthetic promoters. United States Patent No. 6555673 B1
3. Castle LA, Siehl DL, Gorton R, Patten PA, Chen YH, Bertain S, Cho H-J, Duck N, Wong J, Liu D, Lassner MW (2004) Discovery and directed evolution of a glyphosate tolerance gene. Science 304:1151-1154
4. Cuellar W, Gaudin A, Solórzano D, Casas A, Ñopo L, Chudalayandi P, Medrano G, Kreuze J, Ghislain M (2006) Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Plant Mol Biol 62:71-82
5. Dale EC, Ow DW (1990) Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91:79-85
6. Dale EC, Ow DW (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA 88:10558-10562
7. Daley M, Knauf V, Summerfelt KR, Turner JC (1998) Cotransformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep 17:489-496
8. de Frammond A, Back E, Chilton W, Kayes L, Chilton M-D (1986) Two unlinked T-DNAs can transform the same tobacco plant cell and segregate in the F1 generation. Mol Gen Genet 202:125-131
9. Falco SC, Li Z (2000) S-adenosyl-l-methionine synthetase promoter and its use in expression of transgenic genes in plants. US patent publication WO 00/37662
10. Finer JJ, McMullen MD (1991) Transformation of soybean via particle bombardment of embryogenic suspension culture tissue. Vitro Cell Dev Biol-Plant 27:175-182
11. Gleave AP, Miltra DS, Mudge SR, Morris BAM (1999) Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol Biol 40:223-235
12. Hoa TTC, Bong BB, Huq E, Hodge TK (2002) Cre/lox site-specific recombination controls the excision of a transgene from the rice genome. Theor Appl Genet 104:518-525
13. Hoff T, Schnorr KM, Mundy J (2001) A recombinase-mediated transcriptional induction system in transgenic plants. Plant Mol Biol 45:41-49
14. Jia H, Pang Y, Chen X, Fang R (2006) Removal of the selectable marker gene from transgenic tobacco plants by expression of Cre recombinase from a Tobacco Mosaic Virus vector through agroinfection. Trangenic Res 15:375-384
15. Kilby NJ, Davies GJ, Snaith MR (1995) FLP recombinase in transgenic plants: constitutive activity in stably transformed tobacco and generation of marked cell clones in Arabidopsis. Plant J 8:637-652
16. Klimaszewska K, Rutledge RG, Seguin A (2005) Genetic transformation of conifers utilizing somatic embryogenesis. Methods Mol Biol (Clifton, NJ) 286:151-164
17. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10:165-174
18. Kopertekh L, Jüttner J, Schiemann J (2004) Site-specific recombination induced in transgenic plants by PVX virus vector expressing bacteriophage P1 recombinase. Plant Sci 166:485-492
19. Kopertekh L, Schiemann J (2005) Agroinfiltration as a tool for transient expression of cre recombinase in vivo. Trans Res 14:793-798
20. Little EL, Magbanua ZV, Parrott WA (2000) A protocol for repetitive somatic embryogenesis from mature peanut epicotyls. Plant Cell Rep 19:351-357
21. Li Z, Thomas TL (1998) PEI1, an embryo-specific zinc finger protein gene required for heart-stage embryo formation in Arabidopsis. Plant Cell 10:283-398
22. Lu HJ, Zhou XR, Gong ZX, Upadhyaya NM (2001) Generation of selectable marker-free transgenic rice using double right border (DRB) binary vectors. Aust J Plant Physiol 28:241-248
23. Luo H, Lyznik LA, Gidoni D, Hodges TK (2000) FLP-mediated recombination for use in hybrid plant production. Plant J 23:423-430
24. Lutz KA, Bosacchi MH, Maliga P (2006) Plastid marker-gene excision by transiently expressed CRE recombinase. Plant J 45:447-456
25. Maeser S, Kahmann R (1991) The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts. Mol Gen Genet 230:170-176
26. Miki B, McHugh S (2004) Selectable marker genes in transgenic plants: Applications, alternatives and biosafety. J Biotechnol 107:193-232
27. Onouchi H, Yokoi K, Machida C, Matsuzaki H, Oshima Y, Matsuoka K, Nakamura K, Machida Y (1991) Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells. Nucleic Acids Res 19:6373-6378
28. Ow DW (2002) Recombinase-directed plant transformation for the post-genome era. Plant Mol Biol 48:183-200
29. Russell SH, Hoopes JL, Odell JT (1992) Directed excision of a transgene from the plant genome. Mol Gen Genet 234:49-59
30. Sairam R, Chennareddy S, Parani M, Zhang S, Al-Abed D, Abou-Alaiw W, Goldman S (2005) OBPC Symposium: Maize 2004 & beyond-plant regeneration, gene discovery, and genetic engineering of plants for crop improvement. Vitro Cell Dev Biol-Plant 41:411-423
31. Sakhanokho HF, Ozias-Akins P, May OL, Chee PW (2004) Induction of somatic embryogenesis and plant regeneration in selected Georgia and Pee Dee cotton lines. Crop Sci 44:2199-2205
32. Samoylov VM, Tucker DM, Thibaud-Nissen F, Parrott WA (1998) A liquid-medium-based protocol for rapid regeneration from embryogenic soybean cultures. Plant Cell Rep 18:49-54
33. Sreekala C, Wu L, Gu K, Wang D, Tian D, Yin Z (2005) Excision of a selectable marker in transgenic rice (Oryza sativa L.) using a chemically regulated Cre/loxP system. Plant Cell Rep 24:86-94
34. Srivastava V, Anderson OD, Ow DW (1999) Single-copy transgenic wheat generated through the resolution of complex integration patterns. Proc Natl Acad Sci USA 96:11,117-111, 121
35. Stewart CN Jr, Adang MJ, All JN, Boerma HR, Cardineau G, Tucker D, Parrott WA (1996) Genetic transformation, recovery, and characterization of fertile soybean transgenic for a synthetic Bacillus thuringiensis cryIAc gene. Plant Physiol 112:121-129
36. Sugita K, Kasahara T, Matsunaga E, Ebinuma H (2000) A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J 22:461-469
37. Wang Y, Chen B, Hu Y, Li J, Li Z (2005) Inducible excision of selectable marker gene from transgenic plants by the Cre/lox site-specific recombination system. Trans Res 14:605-614
38. Xing A, Zhang Z, Sato S, Staswick P, Clemente T (2000) The use of the two T-DNA binary system to derive marker-free transgenic soybeans. Vitro Cell Dev Biol-Plant 36:456-463
39. Zhang W, Subbarao S, Addae P, Shen A, Armstrong C, Peschke V, Gilbertson L (2003) Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Theor Appl Genet 107:1157-1168
40. Zubko E, Scutt C, Meyer P (2000) Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol 18:442-445
41. Zuo J, Niu QW, Moller SG, Chua NH (2001) Chemical-regulated, site-specific DNA excision in transgenic plants. Nat Biotechnol 19:157-161