A passage through in vitro culture leads to efficient production of marker-free transgenic plants in Brassica juncea using the Cre-loxP system

Transgenic Research

Volumn 16, Issue 6, 2007, Pages 703-712

  Arumugam, N ^a,b   Gupta, Vibha ^a   Jagannath, Arun ^a   Mukhopadhyay, Arundhati ^a   Pradhan, Akshay K ^a   Burma, Pradeep Kumar ^a   Pental, Deepak ^a  

^a UNIVERSITY OF DELHI   (India)

^b PONDICHERRY UNIVERSITY   (India)

Author keywords

Brassica juncea; Cre loxP; Marker free plants; Transgenic crop

Indexed keywords

CRE RECOMBINASE; INTEGRASE;

ARTICLE; BRASSICA; CROSS BREEDING; ENZYMOLOGY; GENETIC ENGINEERING; GENETIC MARKER; GENETICS; TISSUE CULTURE TECHNIQUE; TRANSGENIC PLANT;

BRASSICA; CROSSES, GENETIC; GENETIC ENGINEERING; GENETIC MARKERS; INTEGRASES; PLANTS, GENETICALLY MODIFIED; TISSUE CULTURE TECHNIQUES;

BRASSICA; BRASSICA JUNCEA;

EID: 35848967577     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-006-9058-7     Document Type: Article

References (34)

1. Chen S, Li X, Liu X, Xu H, Meng K, Xiao G, Wei X, Wang F, Zhu Z (2005) Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with double T-DNA binary vector system. Plant Cell Rep 23:625-631
2. Cornielle S, Lutz K, Svab Z, Maliga P (2001) Efficient elimination of selectable marker genes from the plastid genome by CRE-lox site-specific recombination system. Plant J 27:171-178
3. Dale EC, Ow DW (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA 88:10558-10562
4. Dillon PJ, Rosen CA (1990) A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques 9:298-299
5. Gleave AP, Mitra DS, Mudge SR, Morris BAM (1999) Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol Biol 40:223-235
6. Goldsbrough AP, Lastrella CN, Yoder JI (1993) Transposition mediated re-positioning and subsequent elimination of marker genes from transgenic tomato. Bio/technology 11:1286-1292
7. Grover A, Pental D (2003) Breeding objectives and requirements for producing transgenics for major field crops of India. Curr Sci 84:310-320
8. Hajdukiewicz P, Svab Z, Maliga P (1994) The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol 25:939-994
9. st century plant biotechnology. Plant Biotech J 3:141-155
10. Hare PD, Chua N-H (2002) Excision of selectable marker genes from transgenic plants. Nat Biotechnol 20:575-580
11. Hoa TT, Bong BB, Huq E, Hodges TK (2002) Cre-lox site-specific recombination controls the excision of a transgene from the rice genome. Theor Appl Genet 104:518-525
12. Iamtham S, Day A (2000) Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat Biotechnol 18:1172-1176
13. Klimyuk VI, Carroll BJ, Thomas CM, Jones JDG (1993) Alkali treatment for rapid preparation of plant tissue for reliable PCR analysis. Plant J 3:493-494
14. Konig A (2003) A framework for designing transgenic crops-science, safety and citizen's concerns. Nat Biotechnol 21:1274-1279
15. Kumpatla SP, Chandrasekharan MB, Iyer LM, Li G, Hall TC (1998) Genome intruder scanning and modulation systems and transgene silencing. Trends Plant Sci 3:97-104
16. Lyznik LA, Gordon-Kamm WJ, Tao Y (2003) Site-specific recombination for genetic engineering in plants. Plant Cell Rep 21:925-932
17. Mattanovich D, Riiker F, Machado A, Laimer M, Regner F, Steinkellner H, Himmler G, Katinger H (1989) Efficient transformation of Agrobacterium spp by electroporation. Nucleic Acids Res 17:6747
18. Matthews PR, Wang MB, Waterhouse PM, Thornton S, Fieg SJ, Gubler F, Jacobson JV (2001) Marker gene elimination from transgenic barley, using co-transformation with adjacent 'twin T-DNA' on a standard Agrobacterium transformation vector. Mol Breed 7:195-202
19. Mehra S, Pareek A, Bandyopadhyay P, Sharma P, Burma PK, Pental D (2000) Development of transgenics in Indian oilseed mustard (Brassica juncea) resistant to herbicide phosphinotricin. Curr Sci 78:1358-1364
20. Odell J, Caimi P, Sauer B, Russell S (1990) Site-directed recombination in the genome of transgenic tobacco. Mol Gen Genet 223:369-378
21. Ow DW (2002) Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol 48:183-200
22. Park J, Lee YK, Kang BK, Chung WH (2004) Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants. Theor Appl Genet 109:1562-1567
23. Rogers SO, Bendich AJ (1994) Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual, 2nd edn, vol D1. Kluwer Academic Publishers, Dordrecht, The Netherlands, 1-8
24. Rommens CM, Humara JM, Ye J, Yan H, Richael C, Zhang L, Perry R, Swords K (2004) Crop improvement through modification of plant's own genome. Plant Physiol 135:421-431
25. Russel SH, Hoopes JL, Odell JT (1992) Directed excision of a transgene from the plant genome. Mol Gen Genet 243:49-59
26. Salinas J, Matassi G, Montero LM, Bernardi G (1988) Compositional compartmentalization and compositional patterns in nuclear genomes of plant. Nucleic Acids Res 16:4269-4285
27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
28. Sreekala C, Wu L, Gu K, Wang D, Tian D, Yin Z (2005) Excision of selectable marker in transgenic rice (Oryza sativa L.) using a chemically regulated Cre/loxP system. Plant Cell Rep 24:86-94
29. Vaucheret H (1993) Identification of a general silencer for 19S and 35S promoters in a transgenic tobacco plants: 90 bp of homology in the promoter sequence are sufficient for trans-inactivation. CR Acad Sci 316:1471-1483
30. Vergunst AC, Jansen LET, Hooykaas PJJ (1998) Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase. Nucleic Acids Res 26:2729-2734
31. Wang Y, Chen B, Hu Y, Li J, Lin Z (2005) Inducible excision of selectable marker gene from transgenic plants by Cre/lox site-specific recombination system. Transgenic Res 14:605-614
32. Yuan Y, Yun-Jun L, Tao W (2004) A new Cre/lox system for deletion of selectable marker gene. Acta Bot Sin 46:862-866
33. Zhang W, Subbarao S, Addae P, Shen A, Armstrong C, Peschke V, Gilbertson L (2003) Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Theor Appl Genet 107:1157-1168
34. Zuo J, Niu Q-W, Moller SG, Chua N-H (2001) Chemical-regulated, site specific DNA excision in transgenic plants. Nat Biotechnol 19:157-161