Molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested polymerase chain reaction based on 28S ribosomal RNA gene sequences

British Journal of Dermatology

Volumn 161, Issue 5, 2009, Pages 1038-1044

  Ebihara, M ^a,b   Makimura, K ^b   Sato, K ^a,b   Abe, S ^b   Tsuboi, R ^a  

^a TOKYO MEDICAL UNIVERSITY   (Japan)

^b TEIKYO UNIVERSITY   (Japan)

Author keywords

Aspergillus; Candida; Fusarium; Nondermatophyte; Onychomycosis; Polymerase chain reaction

Indexed keywords

FUNGAL DNA; RNA 28S;

ADULT; AGED; ARTICLE; ASPERGILLUS; CANDIDA ALBICANS; CANDIDA TROPICALIS; DERMATOPHYTE; FEMALE; FUNGAL DETECTION; FUSARIUM OXYSPORUM; FUSARIUM SOLANI; FUSARIUM VERTICILLIOIDES; GENE AMPLIFICATION; GENE SEQUENCE; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; NAIL; NUCLEOTIDE SEQUENCE; ONYCHOMYCOSIS; PATHOGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCOPULARIOPSIS BREVICAULIS; SPECIES DIFFERENCE; TRICHOPHYTON MENTAGROPHYTES; TRICHOPHYTON RUBRUM;

ADULT; AGED; AGED, 80 AND OVER; ASPERGILLUS; DNA PRIMERS; DNA, FUNGAL; FEMALE; HUMANS; MALE; MIDDLE AGED; ONYCHOMYCOSIS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 28S; TRICHOPHYTON; YOUNG ADULT;

EID: 71949118067     PISSN: 00070963     EISSN: 13652133     Source Type: Journal    
DOI: 10.1111/j.1365-2133.2009.09249.x     Document Type: Article

References (32)

1. Elewski BE. Onychomycosis: pathogenesis, diagnosis, and management. Clin Microbiol Rev 1998 11 : 415 419.
2. Gupta AK, Jain HC, Lynde CW et al. Prevalence and epidemiology of unsuspected onychomycosis in patients visiting dermatologists' offices in Ontario, Canada-a multicenter survey of 2001 patients. Int J Dermatol 1997 36 : 783 787.
3. Monod M, Baudraz-Rosselet F, Ramelet AA et al. Direct mycological examination in dermatology: a comparison of different methods. Dermatologica 1989 179 : 183 186.
4. Davies RR. Mycological tests and onychomycosis. J Clin Pathol 1968 21 : 729 730.
5. Kardjeva V, Summerbell R, Kantardjiev T et al. Forty-eight-hour diagnosis of onychomycosis with subtyping of Trichophyton rubrum strains. J Clin Microbiol 2006 4 : 1419 1427.
6. Monod M, Bontems O, Zaugg C et al. Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycosis. J Med Microbiol 2006 55 : 1211 1216.
7. De Doncker P, Scher RK, Baran RL et al. Itraconazole therapy is effective for pedal onychomycosis caused by some nondermatophyte molds and mixed infection with dermatophytes and molds: a multicenter study with 36 patients. J Am Acad Dermatol 1997 36 : 173 177.
8. Gianni C, Cerri A, Crosti C. Non-dermatophytic onychomycosis: an underestimated entity? A study of 51 cases. Mycoses 2000 43 : 29 33.
9. Vander Straten MR, Balkis MM, Ghannoum MA. The role of nondermatophyte molds in onychomycosis: diagnosis and treatment. Dermatol Ther 2002 15 : 89 98.
10. Gupta AK, Cooper EA, MacDonald P, Summerbell RC. Utility of inoculum counting (Walshe and English criteria) in clinical diagnosis of onychomycosis caused by nondermatophytic filamentous fungi. J Clin Microbiol 2001 39 : 2115 2121.
11. Liu D, Coloe S, Baird R et al. Molecular determination of dermatophyte fungi using the arbitrarily primed polymerase chain reaction. Br J Dermatol 1997 137 : 351 355.
12. Kamiya A, Kikuchi A, Tomita Y et al. PCR and PCR-RFLP techniques targeting the DNA topoisomerase 2 gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis. J Dermatol Sci 2004 34 : 35 48.
13. Turin L, Riva F, Galbiati G et al. Fast, simple and highly sensitive double-rounded polymerase chain reaction assay to detect medically relevant fungi in dermatological specimens. Eur J Clin Invest 2000 30 : 511 518.
14. Gutzmer R, Mommert S, Küttler U et al. Rapid identification and differentiation of fungal DNA in dermatological specimens by LightCycler PCR. J Med Microbiol 2004 12 (Pt 12 1207 1214.
15. Makimura K, Murayama S, Yamaguchi H. Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 1994 40 : 358 364.
16. Makimura K, Tamura Y, Mochizuki T et al. Phylogenetic classification and species identification of dermatophyte strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 1999 37 : 920 924.
17. Ninet B, Jan I, Bontems O et al. Molecular identification of Fusarium species in onychomycoses. Dermatology 2005 10 : 21 5.
18. Arabatzis M, Bruijnesteijn van Coppenraet LE, Kuijper EJ et al. Diagnosis of common dermatophyte infections by a novel multiplex real-time polymerase chain reaction detection/identification scheme. Br J Dermatol 2007 157 : 681 689.
19. Baek SC, Chae HJ, Houh D et al. Detection and differentiation of causative fungi of onychomycosis using PCR amplification and restriction enzyme analysis. Int J Dermatol 1998 37 : 682 686.
20. Gupta AK, Zaman M, Singh J. Fast and sensitive detection of Trichophyton rubrum DNA from the nail samples of patients with onychomycosis by a double-round polymerase chain reaction-based assay. Br J Dermatol 2007 157 : 698 703.
21. Guého E, Kurtzman CP, Peterson SW. Evolutionary affinities of heterobasidiomycetous yeasts estimated from 18S and 25S ribosomal RNA sequence divergence. Syst Appl Microbiol 1989 12 : 230 236.
22. Ghannoum MA, Hajjeh RA, Scher R et al. A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 2000 43 : 641 648.
23. Gupta AK, Konnukov N, MacDonald P et al. Prevalence and epidemiology of toenail onychomycosis in diabetic subjects: a multicentre survey. Br J Dermatol 1998 139 : 665 667.
24. Midgley G, Moore MK, Cook JC et al. Mycology of nail disorders. J Am Acad Dermatol 1994 31 : S68 74.
25. Tosti A, Piraccini BM, Lorenzi S. Onychomycosis caused by nondermatophytic molds: clinical features and response to treatment of 59 cases. J Am Acad Dermatol 2000 42 : 217 224.
26. Piérard GE, Arrese JE, Doncker PD et al. Present and potential diagnostic techniques in onychomycosis. J Am Acad Dermatol 1996 34 : 273 277.
27. Chun DK, Lee UH, Park HS. Onychomycosis in a premature infant caused by Candida tropicalis. J Eur Acad Dermatol Venereol 2004 18 : 617 618.
28. Ellabib MS, Agaj M, Khalifa Z et al. Yeasts of genus Candida are the dominant cause of onychomycosis in Libyan women but not men: results of a 2-year surveillance study. Br J Dermatol 2002 146 : 1038 1041.
29. Summerbell RC. Epidemiology and ecology of onychomycosis. Dermatology 1997 194 (Suppl. 1 32 6.
30. Loeffler J, Hebart H, Bialek R et al. Contaminations occurring in fungal PCR assays. J Clin Microbiol 1999 37 : 1200 1202.
31. Baran R, Tosti A, Piraccini BM. Uncommon clinical patterns of Fusarium nail infection: report of three cases. Br J Dermatol 1997 136 : 424 427.
32. DiSalvo AF, Fickling AM. A case of nondermatophytic toe onychomycosis caused by Fusarium oxysporum. Arch Dermatol 1980 116 : 699 700.