Contaminations occurring in fungal PCR assays

Journal of Clinical Microbiology

Volumn 37, Issue 4, 1999, Pages 1200-1202

  Loeffler, Juergen ^a,d   Hebart, Holger ^a   Bialek, Ralf ^b   Hagmeyer, Lars ^a   Schmidt, Diethard ^a   Serey, Francois Prâseth ^a   Hartmann, Matthias ^a   Eucker, Jan ^c   Einsele, Hermann ^a  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b Institut für Tropenmedizin Tübingen   (Germany)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d Medizinische Klinik   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA;

ACREMONIUM; AIRBORNE INFECTION; ARTICLE; ASPERGILLUS FUMIGATUS; CONTAMINATION; CONTROLLED STUDY; FUNGUS SPORE; MYCOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

ASPERGILLUS FUMIGATUS; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0032998605     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.4.1200-1202.1999     Document Type: Article

References (18)

1. Arancia, S., S. Sandini, A. Cassone, F. De Bernardis, and R. La Valle. 1997. Construction and use of PCR primers from a 70 kDa heat shock protein gene for identification of Candida albicans. Mol. Cell. Probes 11:329-336.
2. Buchman, T. G., M. Rossier, W. G. Merz, and P. Charache. 1990. Detection of surgical pathogens by in vitro DNA amplification, part 1: rapid identification of Candida albicans by in vitro amplification of a fungus-specific gene. Surgery 108:338-347.
3. Crampin, A. C., and R. C. Matthews. 1993. Application of the polymerase chain reaction to the diagnosis of candidosis by amplification of an HSP 90 gene fragment. J. Med. Microbiol. 39:233-238.
4. Ebeling, W., N. Hennrich, M. Klockow, H. Metz, H. D. Orth, and H. Lang. 1974. Proteinase K from Tritirachium album. Eur. J. Biochem. 47:91-97.
5. Einsele, H., H. Hebart, G. Roller, J. Löffler, I. Rothenhöfer, C. A. Müller, R. A. Bowden, J.-A. van Burik, D. Engelhard, L. Kanz, and U. Schumacher. 1997. Detection and identification of fungal pathogens in blood by using molecular probes. J. Clin. Microbiol. 35:1353-1360.
6. Flahaut, M., D. Sanglard, M. Monod, J. Bille, and M. Rossier. 1998. Rapid detection of Candida albicans in clinical samples by DNA amplification of common regions from C. albicans-secreted aspartic proteinase genes. J. Clin. Microbiol. 36:395-401.
7. Gobet, P., J. C. Buisson, O. Vagner, M. Naciri, M. Grappin, S. Comparot, G. Harly, D. Aubert, I. Varga, P. Camerlynck, and A. Bonnin. 1997. Detection of Cryptosporidium parvum DNA in formed human feces by a sensitive PCR-based assay including uracil-N-glycosylase inactivation. J. Clin. Microbiol. 35:254-256.
8. Jinno, Y., K. Yoshiura, and N. Niikawa. 1990. Use of psoralen as extinguisher of contaminated DNA in PCR. Nucleic Acids Res. 18:6739.
9. Kitamura, K., T. Kaneko, and Y. Yamamoto. 1972. Lysis of viable yeast cells by enzymes of Arthrobacter luteus. J. Gen. Appl. Microbiol. 18:57-71.
10. Kitchin, P. A., Z. Szotyori, C. Fromholc, and N. Almond. 1990. Avoidance of false positives. Nature 344:201.
11. Kwok, S., and R. Higuchi. 1989. Avoiding false positives with PCR. Nature 339:237-238.
12. Löffler, J., H. Hebart, U. Schumacher, H. Reitze, and H. Einsele. 1997. Comparison of different methods for fungal DNA extraction from cultures and blood. J. Clin. Microbiol. 35:3311-3312.
13. Miyakawa, Y., T. Mabuchi, K. Kagaya, and Y. Fukazawa. 1992. Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction. J. Clin. Microbiol. 30:894-900.
14. Müller, F. M., K. E. Werner, M. Kasai, A. Francesconi, S. J. Chanock, and T. J. Walsh. 1998. Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruption. J. Clin. Microbiol. 36:1625-1629.
15. Sarkar, G., and S. Sommer. 1990. Shedding light on PCR contamination. Nature 343:27.
16. Scott, J. H., and R. Schekman. 1980. Lyticase: endoglucanase and protease activities that act together in yeast cell lysis. J. Bacteriol. 142:414-423.
17. Tang, C. M., D. W. Holden, A. Aufauvre-Brown, and J. Cohen. 1993. The detection of Aspergillus spp. by the polymerase chain reaction and its evaluation in bronchoalveolar fluid. Am. Rev. Respir. Dis. 148:1313-1317.
18. Van Burik, J.-A., D. Myerson, R. W. Schreckhise, and R. A. Bowden. 1998. Panfungal PCR assay for detection of fungal infection in human blood specimens. J. Clin. Microbiol. 36:1169-1175.