Fast and sensitive detection of Trichophyton rubrum DNA from the nail samples of patients with onychomycosis by a double-round polymerase chain reaction-based assay

British Journal of Dermatology

Volumn 157, Issue 4, 2007, Pages 698-703

  Gupta, A K ^a,b   Zaman, M ^b   Singh, J ^b  

^a SUNNYBROOK RESEARCH INSTITUTE   (Canada)

^b MEDIPROBE RESEARCH INC   (Canada)

Author keywords

Dermatophyte; Nail; Onychomycosis; Polymerase chain reaction; Trichophyton rubrum

Indexed keywords

ANTIFUNGAL AGENT; CHLORAMPHENICOL; CYCLOHEXIMIDE; GENTAMICIN; POTASSIUM HYDROXIDE;

ARTICLE; DNA DETERMINATION; DNA EXTRACTION; DNA ISOLATION; FEMALE; FINGER NAIL; GENOTYPE; HUMAN; MAJOR CLINICAL STUDY; MALE; ONYCHOMYCOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TRICHOPHYTON RUBRUM;

ACTINS; DNA, FUNGAL; FEMALE; FOOT DERMATOSES; FUNGAL PROTEINS; HUMANS; MALE; MYCOLOGICAL TYPING TECHNIQUES; NAILS; ONYCHOMYCOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRICHOPHYTON;

EID: 34548591653     PISSN: 00070963     EISSN: 13652133     Source Type: Journal    
DOI: 10.1111/j.1365-2133.2007.08110.x     Document Type: Article

References (33)

1. Gupta AK, Jain HC, Lynde CW et al. Prevalence and epidemiology of onychomycosis in patients visiting physicians' offices: a multicenter Canadian survey of 15,000 patients. J Am Acad Dermatol 2000 43 : 244 8.
2. Roberts DT. Prevalence of dermatophyte onychomycosis in the United Kingdom: results of an omnibus survey. Br J Dermatol 1992 126 (Suppl. 39 23 7.
3. Summerbell RC, Kane J, Krajden S. Onychomycosis, tinea pedis and tinea manuum caused by non-dermatophytic filamentous fungi. Mycoses 1989 32 : 609 19.
4. Ohst T, de Hoog S, Presber W et al. Origins of microsatellite diversity in the Trichophyton rubrum-T. violaceum clade (Dermatophytes). J Clin Microbiol 2004 42 : 4444 8.
5. Gupta AK, Scher RK, De Doncker P. Current management of onychomycosis. An overview. Dermatol Clin 1997 15 : 121 35.
6. Gupta AK, Konnikov N, Macdonald P et al. Prevalence and epidemiology of toenail onychomycosis in diabetic subjects: a multicentre survey. Br J Dermatol 1998 139 : 665 71.
7. Evans EG. Causative pathogens in onychomycosis and the possibility of treatment resistance: a review. J Am Acad Dermatol 1998 38 : S32 6.
8. Anane S, Aoun K, Zallagua N et al. Onychomycosis in Tunis area: epidemiological and mycological data. Ann Dermatol Venereol 2001 128 : 733 6.
9. Baudraz-Rosselet F, Frenk E. In vitro nail invasion by pathogenic and non-pathogenic fungi under different culture conditions. Mycoses 1990 33 : 553 7.
10. Lateur N, Mortaki A, Andre J. Two hundred ninety-six cases of onychomycosis in children and teenagers: a 10-year laboratory survey. Pediatr Dermatol 2003 20 : 385 8.
11. Reisberger EM, Abels C, Landthaler M et al. Histopathological diagnosis of onychomycosis by periodic acid-Schiff-stained nail clippings. Br J Dermatol 2003 148 : 749 54.
12. Weinberg JM, Koestenblatt EK, Jennings MB. Utility of histopathologic analysis in the evaluation of onychomycosis. J Am Podiatr Med Assoc 2005 95 : 258 63.
13. Zabawski EJ Jr., Styles AR, Cockerell CJ. Routine periodic acid-Schiff staining of nail plate fragments in fungal cultures for onychomycosis: a method to increase the sensitivity of diagnosis. Cutis 2000 66 : 456 8.
14. Clayton YM. Clinical and mycological diagnostic aspects of onychomycoses and dermatomycoses. Clin Exp Dermatol 1992 17 (Suppl. 1 37 40.
15. Fletcher CL, Hay RJ, Smeeton NC. Onychomycosis: the development of a clinical diagnostic aid for toenail disease. Part I. Establishing discriminating historical and clinical features. Br J Dermatol 2004 150 : 701 5.
16. Lawry MA, Haneke E, Strobeck K et al. Methods for diagnosing onychomycosis: a comparative study and review of the literature. Arch Dermatol 2000 136 : 1112 16.
17. Midgley G, Moore MK, Cook JC et al. Mycology of nail disorders. J Am Acad Dermatol 1994 31 : S68 74.
18. Arca E, Saracli MA, Akar A et al. Polymerase chain reaction in the diagnosis of onychomycosis. Eur J Dermatol 2004 14 : 52 5.
19. Kardjeva V, Summerbell R, Kantardjiev T et al. Forty-eight-hour diagnosis of onychomycosis with subtyping of Trichophyton rubrum strains. J Clin Microbiol 2006 44 : 1419 27.
20. Anderson TD, Ross JP, Roby RK et al. A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework. J Forensic Sci 1999 44 : 1053 6.
21. Okeke CN, Tsuboi R, Kawai M et al. Isolation of an intron-containing partial sequence of the gene encoding dermatophyte actin (ACT) and detection of a fragment of the transcript by reverse transcription-nested PCR as a means of assessing the viability of dermatophytes in skin scales. J Clin Microbiol 2001 39 : 101 6.
22. Kawai M. [Diagnosis of dermatophytoses: conventional methods and recent molecular biological methods]. Nippon Ishinkin Gakkai Zasshi 2003 44 : 261 4.
23. Tsuboi R, Okeke CN, Inoue A et al. Identification and viability assessment of dermatophytes infecting nail based on quantitative PCR of dermatophyte actin (ACT) mRNA. Nippon Ishinkin Gakkai Zasshi 2002 43 : 91 3.
24. Graser Y, el Fari M, Presber W et al. Identification of common dermatophytes (Trichophyton, Microsporum, Epidermophyton) using polymerase chain reactions. Br J Dermatol 1998 138 : 576 82.
25. Harmsen D, Schwinn A, Brocker EB et al. Molecular differentiation of dermatophyte fungi. Mycoses 1999 42 : 67 70.
26. Jackson CJ, Barton RC, Evans EG. Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J Clin Microbiol 1999 37 : 931 6.
27. Kano R, Hirai A, Muramatsu M et al. Direct detection of dermatophytes in skin samples based on sequences of the chitin synthase 1 (CHS1) gene. J Vet Med Sci 2003 65 : 267 70.
28. Makimura K, Tamura Y, Mochizuki T et al. Phylogenetic classification and species identification of dermatophyte strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 1999 37 : 920 4.
29. Shin JH, Sung JH, Park SJ et al. Species identification and strain differentiation of dermatophyte fungi using polymerase chain reaction amplification and restriction enzyme analysis. J Am Acad Dermatol 2003 48 : 857 65.
30. Turin L, Riva F, Galbiati G et al. Fast, simple and highly sensitive double-rounded polymerase chain reaction assay to detect medically relevant fungi in dermatological specimens. Eur J Clin Invest 2000 30 : 511 18.
31. Faggi E, Pini G, Campisi E et al. Application of PCR to distinguish common species of dermatophytes. J Clin Microbiol 2001 39 : 3382 5.
32. Kamiya A, Kikuchi A, Tomita Y et al. PCR and PCR-RFLP techniques targeting the DNA topoisomerase II gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis. J Dermatol Sci 2004 34 : 35 48.
33. Liu D, Pearce L, Lilley G et al. PCR identification of dermatophyte fungi Trichophyton rubrum, T. soudanense and T. gourvilii. J Med Microbiol 2002 51 : 117 22.