An improved method for the isolation of total RNA from Malva pusilla tissues infected with Colletotrichum gloeosporioides

Journal of Phytopathology

Volumn 148, Issue 1, 2000, Pages 57-60

  Chen, G Y J ^a   Jin, S ^a   Goodwin, P H ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Colletotrichum gloeosporioides; Mallow; Polyvinylpolypyrrolidone (PVPP); Polyvinylpyrrolidone (PVP)

Indexed keywords

EXTRACTION METHOD; FUNGUS; PATHOGEN; RNA;

COLLETOTRICHUM GLOSOSPORIOIDES; MALVA PUSILLA;

EID: 0033997843     PISSN: 09311785     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1439-0434.2000.00470.x     Document Type: Article

References (16)

1. Birnboim, H. C. (1992): Extraction of high molecular weight RNA and DNA from cultured mammalian cells. Meth Enzymol 216, 154-160.
2. Chang, S., J. Puryear, J. Cairney (1993): A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol. Rep 11, 113-116.
3. Chirgwin, J. M., A. E. Przybyla, R. J. MacDonald, W. J. Rutter (1979): Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
4. Chomczynski, P., N. Sacchi (1987): Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162, 156-159.
5. Dong, J., D. I. Dunstan (1996): A reliable method for extraction of RNA from various conifer tissues. Plant Cell Rep 15, 516-521.
6. Duan, J., X. Cai, L. Zhou, J. Wang (1997): Single-step method of total RNA isolation by sodium dodecyl sulfate phenol extraction from cultured cells. Anal Biochem. 251, 291-292.
7. Hale, A. D., J. Green, D. W. G. Brown (1996): Comparison of four RNA extraction methods for the detection of small round structured viruses in faecal specimens. J. Virol. Methods 57, 195-201.
8. Katterman, F. R. H., V. I. Shattuck (1983): An effective method of DNA isolation from the mature leaves of Gossypium species that contain large amounts of phenolic terpenoids and tannins. Prep Biochem. 13, 347-359.
9. Liu, Z., D. B. Batt, G. G. Carmichael (1994): An improved rapid method of isolating RNA from cultured cells by SDS-acid phenol chloroform extraction. Biotechniques 16, 56-57.
10. Logemann, J., J. Schell, L. Willmitzer (1987): Improved method for the isolation of RNA from plant tissues. Anal Biochem. 163, 16-20.
11. MacKenzie, D. J., M. A. McLean, S. Mukerji, M. Green (1997): Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81, 222-226.
12. Noonberg, S. B., G. K. Scott, C. C. Benz (1995): Effect of pH on RNA degradation during guanidinium extraction. Biotechniques 19, 731-733.
13. Sambrook, J., E. F. Fritsch, T. Maniatis (1989): Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
14. Schneiderbauer, A., H. J. Sandermann, D. Ernst (1991): Isolation of functional RNA from plant tissues rich in phenolic compounds. Anal Biochem. 197, 91-95.
15. Tse, C., J. Etienne (1995): Improved high-quality DNA extraction with RNA, DNA, and protein from tissue samples. Meth Mol Cell Biol. 5, 373-376.
16. Weber, K., M. E. Bolander, G. Sarkar (1998): PIG-B: a homemade monophasic cocktail for the extraction of RNA. Mol Biotech 9, 73-77.