A sandwich ELISA for the detection of Wnt5a

Journal of Immunological Methods

Volumn 352, Issue 1-2, 2010, Pages 38-44

  Kummitha, China Malakondaiah ^a   Mayle, Kristine M ^a   Christman II, Mark A ^a   Deosarkar, Sudhir P ^a   Schwartz, Anthony L ^b   McCall, Kelly D ^b   Kohn, Leonard D ^b   Malgor, Ramiro ^b   Goetz, Douglas J ^a,b  

^a US Institute of Navigation   (United States)

^b OHIO UNIVERSITY   (United States)

Author keywords

Cancer; Diagnostics; Inflammation; Sandwich ELISA; Wnt; Wnt5a

Indexed keywords

AVIDIN; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN G; MACROGOL; PROTEIN ANTIBODY; STREPTAVIDIN; WNT5A PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DETERMINATION; SENSITIVITY AND SPECIFICITY; SIGNAL DETECTION;

ANIMALS; ANTIGEN-ANTIBODY REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EQUIDAE; GOATS; HUMANS; MICE; PROTEIN BINDING; RABBITS; SENSITIVITY AND SPECIFICITY; WNT PROTEINS;

ARMORACIA RUSTICANA; BOVINAE; CAPRA HIRCUS; EQUUS ASINUS; ORYCTOLAGUS CUNICULUS;

EID: 71749090991     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.11.005     Document Type: Article

References (26)

1. Christman II M.A., Goetz D.J., Dickerson E., McCall K.D., Lewis C.J., Benencia F., Silver M.J., Kohn L.D., and Malgor R. Wnt5a is expressed in murine and human atherosclerotic lesions. Am. J. Physiol., Heart Circ. Physiol. 294 (2008) H2864
2. Clark C.C., Cohen I., Eichstetter I., Cannizzaro L.A., McPherson J.D., Wasmuth J.J., and Iozzo R.V. Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (Wnt5A) to chromosome 3p14-p21. Genomics 18 (1993) 249
3. Clevers H. Wnt/beta-catenin signaling in development and disease. Cell 127 (2006) 469
4. Crowther J.R. The ELISA guidebook. Methods Mol. Biol. 149 III-IV (2000) 1
5. Da Forno P.D., Pringle J.H., Hutchinson P., Osborn J., Huang Q., Potter L., Hancox R.A., Fletcher A., and Saldanha G.S. Wnt5A expression increases during melanoma progression and correlates with outcome. Clin. Cancer Res. 14 (2008) 5825
6. Dagia N.M., Harii N., Meli A.E., Sun X., Lewis C.J., Kohn L.D., and Goetz D.J. Phenyl methimazole inhibits TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and reduces monocytic cell adhesion to endothelial cells. J. Immunol. 173 (2004) 2041
7. Dejmek J., Dejmek A., Safholm A., Sjolander A., and Andersson T. Wnt-5a protein expression in primary dukes B colon cancers identifies a subgroup of patients with good prognosis. Cancer Res. 65 (2005) 9142
8. George S.J. Wnt pathway: a new role in regulation of inflammation. Arterioscler. Thromb. Vasc. Biol. 28 (2008) 400
9. Hausmann G., Banziger C., and Basler K. Helping wingless take flight: how Wnt proteins are secreted. Nat. Rev., Mol. Cell Biol. 8 (2007) 331
10. Kuhl M., Sheldahl L.C., Park M., Miller J.R., and Moon R.T. The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape. Trends Genet. 16 (2000) 279
11. Kurayoshi M., Oue N., Yamamoto H., Kishida M., Inoue A., Asahara T., Yasui W., and Kikuchi A. Expression of Wnt-5a is correlated with aggressiveness of gastric cancer by stimulating cell migration and invasion. Cancer Res. 66 (2006) 10439
12. Liu X.H., Pan M.H., Lu Z.F., Wu B., Rao Q., Zhou Z.Y., and Zhou X.J. Expression of Wnt-5a and its clinicopathological significance in hepatocellular carcinoma. Dig. Liver Dis. 40 (2008) 560
13. Lizana J., and Hellsing K. Polymer enhancement of automated immunological nephelometric analysis, as illustrated by determination of urinary albumin. Clin. Chem. 20 (1974) 415
14. Logan C.Y., and Nusse R. The Wnt signaling pathway in development and disease. Annu. Rev. Cell Dev. Biol. 20 (2004) 781
15. Nusse R. Wnt signaling in disease and in development. Cell Res. 15 (2005) 28
16. Ochi Y., Kajita Y., Inui T., Yamashiro K., Takasu N., Sato Y., and Nagata A. Sensitive thyroid-stimulating antibody assay in whole serum containing five percent polyethylene glycol using porcine thyroid cells. Thyroid 10 (2000) 653
17. Pereira C., Schaer D.J., Bachli E.B., Kurrer M.O., and Schoedon G. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler. Thromb. Vasc. Biol. 28 (2008) 504
18. Pilarsky C., Ammerpohl O., Sipos B., Dahl E., Hartmann A., Wellmann A., Braunschweig T., Lohr M., Jesnowski R., Friess H., Wente M.N., Kristiansen G., Jahnke B., Denz A., Ruckert F., Schackert H.K., Kloppel G., Kalthoff H., Saeger H.D., and Grutzmann R. Activation of Wnt signalling in stroma from pancreatic cancer identified by gene expression profiling. J. Cell. Mol. Med. 12 (2008) 2823
19. Pukrop T., Klemm F., Hagemann T., Gradl D., Schulz M., Siemes S., Trumper L., and Binder C. Wnt 5a signaling is critical for macrophage-induced invasion of breast cancer cell lines. Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 5454
20. Reischl J., Schwenke S., Beekman J.M., Mrowietz U., Sturzebecher S., and Heubach J.F. Increased expression of Wnt5a in psoriatic plaques. J. Invest. Dermatol. 127 (2007) 163
21. Sen M., Lauterbach K., El-Gabalawy H., Firestein G.S., Corr M., and Carson D.A. Expression and function of wingless and frizzled homologs in rheumatoid arthritis. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 2791
22. Strasser R.H., and Lefkowitz R.J. Homologous desensitization of beta-adrenergic receptor coupled adenylate cyclase. Resensitization by polyethylene glycol treatment. J. Biol. Chem. 260 (1985) 4561
23. van Amerongen R., Mikels A., and Nusse R. Alternative Wnt signaling is initiated by distinct receptors. Sci. Signal. 1 (2008) re9
24. Watanabe Y., Tada H., Hidaka Y., Takano T., Takeoka K., Fukata S., Kuma K., and Amino N. Polyethylene glycol increases the detection of anti-thyrotropin receptor antibodies by a radioreceptor assay. Clin. Chem. 45 (1999) 407
25. Weeraratna A.T., Jiang Y., Hostetter G., Rosenblatt K., Duray P., Bittner M., and Trent J.M. Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma. Cancer Cell 1 (2002) 279
26. Yolken R.H. Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev. Infect. Dis. 4 (1982) 35