A cell-based assay for screening lipoxygenase inhibitors

Prostaglandins and Other Lipid Mediators

Volumn 90, Issue 3-4, 2009, Pages 98-104

  Nair, Dileep G ^a   Funk, Colin D ^a  

^a QUEEN S UNIVERSITY   (Canada)

Author keywords

Cell based; Fluorescence assay; Lipoxygenase inhibitors

Indexed keywords

LIPOXYGENASE INHIBITOR;

ARTICLE; CELL FUNCTION; CONTROLLED STUDY; DRUG INDUSTRY; DRUG INHIBITION; FLUORESCENCE; HUMAN; HUMAN CELL; IN VIVO STUDY; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION;

ACETAMIDES; CELL LINE; DRUG EVALUATION, PRECLINICAL; FLUORENES; HUMANS; HYDROXYUREA; INHIBITORY CONCENTRATION 50; LIPOXYGENASE; LIPOXYGENASE INHIBITORS; MICROSCOPY, FLUORESCENCE; NORDIHYDROGUAIARETIC ACID;

EID: 71549127132     PISSN: 10988823     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.prostaglandins.2009.09.006     Document Type: Article

References (37)

1. Kuhn H., Saam J., Eibach S., Holzhutter H.G., Ivanov I., and Walther M. Structural biology of mammalian lipoxygenases: enzymatic consequences of targeted alterations of the protein structure. Biochem Biophys Res Commun 338 (2005) 93-101
2. Brash A.R. Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate. J Biol Chem 274 (1999) 23679-23682
3. Peters-Golden M., and Brock T.G. Intracellular compartmentalization of leukotriene synthesis: unexpected nuclear secrets. FEBS Lett 487 (2001) 323-326
4. Chen X.S., and Funk C.D. The N-terminal "beta-barrel" domain of 5-lipoxygenase is essential for nuclear membrane translocation. J Biol Chem 276 (2001) 811-818
5. Funk C.D. Leukotriene modifiers as potential therapeutics for cardiovascular disease. Nat Rev Drug Discov 4 (2005) 664-672
6. Funk C.D. Prostaglandins leukotrienes: advances in eicosanoid biology. Science 294 (2001) 1871-1875
7. Aleem A.M., Jankun J., Dignam J.D., et al. Human platelet 12-lipoxygenase, new findings about its activity, membrane binding and low-resolution structure. J Mol Biol 376 (2008) 193-209
8. Johnson E.N., Nanney L.B., Virmani J., Lawson J.A., and Funk C.D. Basal transepidermal water loss is increased in platelet-type 12-lipoxygenase deficient mice. J Invest Dermatol 112 (1999) 861-865
9. Hussain H., Shornick L.P., Shannon V.R., et al. Epidermis contains platelet-type 12-lipoxygenase that is overexpressed in germinal layer keratinocytes in psoriasis. Am J Physiol 266 (1994) C243-C253
10. Johnson E.N., Brass L.F., and Funk C.D. Increased platelet sensitivity to ADP in mice lacking platelet-type 12-lipoxygenase. Proc Natl Acad Sci USA 95 (1998) 3100-3105
11. Donowitz M. Arachidonic acid metabolites and their role in inflammatory bowel disease. An update requiring addition of a pathway. Gastroenterology 88 (1985) 580-587
12. Metzger K., Angres G., Maier H., and Lehmann W.D. Lipoxygenase products in human saliva: patients with oral cancer compared to controls. Free Radic Biol Med 18 (1995) 185-194
13. Kuhn H., Walther M., and Kuban R.J. Mammalian arachidonate 15-lipoxygenases structure, function, and biological implications. Prostaglandins Other Lipid Mediat 68-69 (2002) 263-290
14. Skrzypczak-Jankun E., Chorostowska-Wynimko J., Selman S.H., and Jankun J. Lipoxygenases-a challenging problem in enzyme inhibition and drug development. Curr Enzyme Inhib 3 (2007) 119-132
15. Pufahl R.A., Kasten T.P., Hills R., et al. Development of a fluorescence-based enzyme assay of human 5-lipoxygenase. Anal Biochem 364 (2007) 204-212
16. Auerbach B.J., Kiely J.S., and Cornicelli J.A. A spectrophotometric microtiter-based assay for the detection of hydroperoxy derivatives of linoleic acid. Anal Biochem 201 (1992) 375-380
17. Kratky D., Lass A., Abuja P.M., Esterbauer H., and Kuhn H. A sensitive chemiluminescence method to measure the lipoxygenase catalyzed oxygenation of complex substrates. Biochim Biophys Acta 1437 (1999) 13-22
18. Poeckel D., Zemski Berry K.A., Murphy R.C., and Funk C.D. Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in ApoE knock-out mice. J Biol Chem 284 (2009) 21077-21089
19. Chen X.S., Brash A.R., and Funk C.D. Purification and characterization of recombinant histidine-tagged human platelet 12-lipoxygenase expressed in a baculovirus/insect cell system. Eur J Biochem 214 (1993) 845-852
20. Seta F., Chung A.D., Turner P.V., Mewburn J.D., Yu Y., and Funk C.D. Renal and cardiovascular characterization of COX-2 knockdown mice. Am J Physiol Regul Integr Comp Physiol 296 (2009) R1751-R1760
21. Hu T., Ramachandrarao S.P., Siva S., et al. Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells. Am J Physiol Renal Physiol 289 (2005) F816-F825
22. 2+ and differentially interact with platelet-type 12-lipoxygenase. Mol Pharmacol 70 (2006) 1071-1078
23. Mbonye U.R., Wada M., Rieke C.J., Tang H.Y., Dewitt D.L., and Smith W.L. The 19-amino acid cassette of cyclooxygenase-2 mediates entry of the protein into the endoplasmic reticulum-associated degradation system. J Biol Chem 281 (2006) 35770-35778
24. Halliwell B., and Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. Br J Pharmacol 142 (2004) 231-255
25. Kamp F., Zakim D., Zhang F., Noy N., and Hamilton J.A. Fatty acid flip-flop in phospholipid bilayers is extremely fast. Biochemistry 34 (1995) 11928-11937
26. Berk P.D., Zhou S.L., Stump D., Kiang C.L., and Isola L.M. Recent studies of the cellular uptake of long chain free fatty acids. Trans Am Clin Climatol Assoc 105 (1994) 179-189
27. Glick J., Santoyo G., and Casey P.J. Arachidonate and related unsaturated fatty acids selectively inactivate the guanine nucleotide-binding regulatory protein, gz. J Biol Chem. 271 (1996) 2949-2954
28. Brash A.R., and Ingram C.D. Lipoxygenase metabolism of endogenous and exogenous arachidonate in leukocytes: GC-MS analyses of incubations in H2(18)O buffers. Prostaglandins Leukot Med 23 (1986) 149-154
29. Reddanna P., Rao M.K., and Reddy C.C. Inhibition of 5-lipoxygenase by vitamin E. FEBS Lett 193 (1985) 39-43
30. You K.M., Jong H.G., and Kim H.P. Inhibition of cyclooxygenase/lipoxygenase from human platelets by polyhydroxylated/methoxylated flavonoids isolated from medicinal plants. Arch Pharm Res 22 (1999) 18-24
31. Carroll J., Jonsson E.N., Ebel R., Hartman M.S., Holman T.R., and Crews P. Probing sponge-derived terpenoids for human 15-lipoxygenase inhibitors. J Org Chem 66 (2001) 6847-6851
32. Summers J.B., Gunn B.P., Martin J.G., et al. Orally active hydroxamic acid inhibitors of leukotriene biosynthesis. J Med Chem 31 (1988) 3-5
33. Bell R.L., Bouska J.B., Malo P.E., et al. Optimization of the potency and duration of action of N-hydroxyurea 5-lipoxygenase inhibitors. J Pharmacol Exp Ther 272 (1995) 724-731
34. Nie D., Tang K., Diglio C., and Honn K.V. Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase. Blood 95 (2000) 2304-2311
35. Bocan T.M., Rosebury W.S., Mueller S.B., et al. A specific 15-lipoxygenase inhibitor limits the progression and monocyte-macrophage enrichment of hypercholesterolemia-induced atherosclerosis in the rabbit. Atherosclerosis 136 (1998) 203-216
36. Kim S.Y., Kim T.B., Moon K.A., et al. Regulation of pro-inflammatory responses by lipoxygenases via intracellular reactive oxygen species in vitro and in vivo. Exp Mol Med 40 (2008) 461-476
37. Waslidge N.B., and Hayes D.J. A colorimetric method for the determination of lipoxygenase activity suitable for use in a high throughput assay format. Anal Biochem 231 (1995) 354-358