A novel economic method for high throughput production of recombinant baculovirus by infecting insect cells with Bacmid-containing diminopimelate-auxotrophic Escherichia coli

Journal of Biotechnology

Volumn 145, Issue 1, 2010, Pages 23-29

  Yao, Lun guang ^a   Sun, Jing chen ^a,b   Xu, Hua ^a   Kan, Yun chao ^a   Zhang, Xiang man ^a   Yan, Hui Chao ^b  

^a Nan Yang Normal University Rothamsted Research Joint Laboratory of Insect Biology   (China)

^b SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Diaminopimelate auxotrophy; High throughput; Invasion factor; Recombinant baculovirus

Indexed keywords

AUXOTROPHY; BACTERIAL CELLS; BACULOVIRUS; CELL WALLS; COST-EFFECTIVE METHODS; E. COLI; HIGH THROUGHPUT; INSECT CELLS; LABOUR-INTENSIVE; RECOMBINANT BACULOVIRUS; SPODOPTERA FRUGIPERDA; TRANSFECTION REAGENTS;

BACTERIOLOGY; CELLS; DNA; ESCHERICHIA COLI; GENES; NUCLEIC ACIDS;

THROUGHPUT;

BACTERIAL DNA; DIAMINOPIMELIC ACID; VIRUS DNA;

ARTICLE; AUXOTROPHY; BACTERIAL CELL WALL; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; DNA TRANSPOSITION; ECONOMIC ASPECT; ESCHERICHIA COLI; GENETIC TRANSFECTION; INSECT CELL; LEPIDOPTERA; NONHUMAN; PRIORITY JOURNAL; TIME; VIRUS PARTICLE; VIRUS RECOMBINANT; YERSINIA PSEUDOTUBERCULOSIS;

ANIMALS; BACULOVIRIDAE; CLONING, MOLECULAR; DIAMINOPIMELIC ACID; DNA, RECOMBINANT; ESCHERICHIA COLI; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; SPODOPTERA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HEXAPODA; SPODOPTERA FRUGIPERDA; YERSINIA;

EID: 71449094924     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.10.003     Document Type: Article

References (18)

1. Airenne K.J., Peltomaa E., Hytönen V.P., Laitinen O.H., and Ylä-Herttuala S. Improved generation of recombinant baculovirus genomes in Escherichia coli. Nucleic Acids Res. 31 17 (2003) e101
2. Berger I., Fitzgerald D.J., and Richmond T.J. Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol. 22 (2004) 1583-1587
3. Cherepanov P.P., and Wackernagel W. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158 (1995) 9-14
4. Grillot-Courvalin C., Goussard S., Huetz F., Ojcius D.M., and Courvalin P. Functional gene transfer from intercellular bacteria to mammalian cells. Nat. Biotechnol. 16 (1998) 862-866
5. Haziza C., Stragier P., and Patte J.C. Nucleotide sequence of the asd gene of Escherichia coli: absence of a typical attenuation signal. EMBO J. 1 (1982) 379-384
6. Horn C., and Wimmer E.A. A versatile vector set for animal transgenesis. Dev. Genes Evol. 210 (2000) 630-637
7. Kost T.A., Condreay J.P., and Jarvis D.L. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat. Biotechnol. 23 (2005) 567-575
8. Li M.Z., and Elledge S.J. MAGIC, an in vivo genetics method for the rapid construction of recombinant DNA moleculars. Nat. Genet. 37 (2005) 311-319
9. Luckow V.A., Lee S.C., and Barry G.F. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67 (1993) 4566-4579
10. Narayanan K., and Warburton P.E. DNA modification and functional delivery into human cells using Escherichia coli DH10B. Nucleic Acids Res. 31 9 (2003) e51
11. Sambrook J., and Russell D.W. Cloning Molecular. A Laboratory Manual (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
12. Smith G.E., Summers M.D., and Fraser M.J. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3 (1983) 2156-2165
13. Sun J.C., Zhang E.H., Yao L.G., Zhang H.L., and Jin P.F. A high efficient method of constructing recombinant Bombyx mori (silkworm) multiple nucleopolyhedrovirus based on zero-background Tn7-mediated transposition in Escherichia coli. Biotechnol. Prog. 25 (2009) 524-529
14. Susan G.M., and Linda A.K. Efficient transfection of insect cells with baculovirus DNA using electroporation. J. Gen. Virol. 70 (1989) 3501-3505
15. Yao L.G., Liu Z.C., Zhang X.M., Kan Y.C., and Zhou J.J. A highly efficient method for the generation of a recombinant Bombyx mori nuclear-polyhedrosis-virus Bacmid and large-scale expression of foreign proteins in silkworm (B. mori) larvae. Biotechnol. Appl. Biochem. 48 (2007) 45-53
16. Yao N., Yao L.G., Kan Y.C., Zhou W.K., Q.I., and Y.P. Improvement of baculovirus expression system and purification of IL-6 protein expressed in insect cells. Chin. J. Biotechnol. 22 (2006) 572-580
17. Zhao Y.G., Chapman D.A.G., and Jones L.M. Improving baculovirus recombination. Nucleic Acids Res. 31 2 (2003) e6
18. Zhou W.K., Yao L.G., Xu H., Yan F., and Qi Y.P. The function of envelope protein P74 from Autographa californica multiple nucleopolyhedrovirus in primary infection to host. Virus Genes 30 (2005) 139-150